BBa_K887072
type: ComponentDefinition
persistent identity: BBa K887072
display id: BBa_K887072
version: 1
was generated by: iGEM to SBOL conversion
description: Plac+B0034+zif268+alsS+B0034+PBSII+ilvC+B0034+HIVC+ilvD
created: 2012-09-13T11:00:00Z
modified: 2015-05-08T01:13:40Z
mutable provenance: This biobrick is composed of BBa_K887040, BBa_B0034, BBa_K887020,BBa_B003 and BBa_K887023.
owned by: james
owned by: myers
mutable description: This part contains three zinc fingers and following enzymes. These three enzymes will change pyruvate to 2-ketoisoalerate(the Intermediate of isobutanol). With additional zinc finger, these fusion protein can bind to the DNA program according to the order.These strategy can enhance productivity theoretically.
discontinued: false
dominant: false
group u list: _1150_
m user id: 0
owner id: 14115
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Nothing special, the only problem is this part is a little big.
star: false
creator: TSAI,LU-AN
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K887023
component: BBa_B0034
component: BBa_K887020
component: BBa_K887040
sequence annotation: BBa_K887040
sequence annotation: BBa_B0034
sequence annotation: BBa_K887020
sequence annotation: BBa_K887023
sequence: https://synbiohub.org/public/igem/BBa_K887072_sequence/1

BBa_K887023
type: Component
persistent identity: component2182967
display id: component2182967
version: 1
top level: BBa_K887072
definition: BBa_K887023
access: public

BBa_B0034
type: Component
persistent identity: component2182963
display id: component2182963
version: 1
top level: BBa_K887072
definition: BBa_B0034
access: public

BBa_K887020
type: Component
persistent identity: component2182961
display id: component2182961
version: 1
top level: BBa_K887072
definition: BBa_K887020
access: public

BBa_B0034
type: Component
persistent identity: component2182957
display id: component2182957
version: 1
top level: BBa_K887072
definition: BBa_B0034
access: public

BBa_K887040
type: Component
persistent identity: component2182955
display id: component2182955
version: 1
top level: BBa_K887072
definition: BBa_K887040
access: public

BBa_K887040
type: SequenceAnnotation
persistent identity: annotation2182955
display id: annotation2182955
version: 1
top level: BBa_K887072
location: https://synbiohub.org/public/igem/BBa_K887072/annotation2182955/range2182955/1
component: BBa_K887040

https://synbiohub.org/public/igem/BBa_K887072/annotation2182955/range2182955/1
type: Range
persistent identity: range2182955
display id: range2182955
version: 1
top level: BBa_K887072
direction: direction/reverse
start: 1
end: 2244
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2182957
display id: annotation2182957
version: 1
top level: BBa_K887072
location: https://synbiohub.org/public/igem/BBa_K887072/annotation2182957/range2182957/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K887072/annotation2182957/range2182957/1
type: Range
persistent identity: range2182957
display id: range2182957
version: 1
top level: BBa_K887072
direction: direction/reverse
start: 2253
end: 2264
orientation: inline

BBa_K887020
type: SequenceAnnotation
persistent identity: annotation2182961
display id: annotation2182961
version: 1
top level: BBa_K887072
location: https://synbiohub.org/public/igem/BBa_K887072/annotation2182961/range2182961/1
component: BBa_K887020

https://synbiohub.org/public/igem/BBa_K887072/annotation2182961/range2182961/1
type: Range
persistent identity: range2182961
display id: range2182961
version: 1
top level: BBa_K887072
direction: direction/reverse
start: 2273
end: 4042
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2182963
display id: annotation2182963
version: 1
top level: BBa_K887072
location: https://synbiohub.org/public/igem/BBa_K887072/annotation2182963/range2182963/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K887072/annotation2182963/range2182963/1
type: Range
persistent identity: range2182963
display id: range2182963
version: 1
top level: BBa_K887072
direction: direction/reverse
start: 4051
end: 4062
orientation: inline

BBa_K887023
type: SequenceAnnotation
persistent identity: annotation2182967
display id: annotation2182967
version: 1
top level: BBa_K887072
location: https://synbiohub.org/public/igem/BBa_K887072/annotation2182967/range2182967/1
component: BBa_K887023

https://synbiohub.org/public/igem/BBa_K887072/annotation2182967/range2182967/1
type: Range
persistent identity: range2182967
display id: range2182967
version: 1
top level: BBa_K887072
direction: direction/reverse
start: 4071
end: 6179
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_K887040
type: ComponentDefinition
persistent identity: BBa K887040
display id: BBa_K887040
version: 1
was generated by: iGEM to SBOL conversion
description: Plac+B0034+zif268+alss
created: 2012-08-29T11:00:00Z
modified: 2015-05-08T01:13:40Z
mutable provenance: qwq
owned by: james
owned by: myers
mutable description: plac is a promoter regulated by lacI repressor.B0034 is and strong RBS which can be bond by ribosome XD to initiate translation.
discontinued: false
dominant: false
group u list: _1150_
m user id: 0
owner id: 14159
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: qwe
star: false
creator: Weiting Chen
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K539713
component: BBa_K323105
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K323105
sequence annotation: BBa_K539713
sequence: https://synbiohub.org/public/igem/BBa_K887040_sequence/1

BBa_K539713
type: Component
persistent identity: component2182505
display id: component2182505
version: 1
top level: BBa_K887040
definition: BBa_K539713
access: public

BBa_K323105
type: Component
persistent identity: component2182503
display id: component2182503
version: 1
top level: BBa_K887040
definition: BBa_K323105
access: public

BBa_B0034
type: Component
persistent identity: component2182502
display id: component2182502
version: 1
top level: BBa_K887040
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component2182494
display id: component2182494
version: 1
top level: BBa_K887040
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2182494
display id: annotation2182494
version: 1
top level: BBa_K887040
location: https://synbiohub.org/public/igem/BBa_K887040/annotation2182494/range2182494/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K887040/annotation2182494/range2182494/1
type: Range
persistent identity: range2182494
display id: range2182494
version: 1
top level: BBa_K887040
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2182502
display id: annotation2182502
version: 1
top level: BBa_K887040
location: https://synbiohub.org/public/igem/BBa_K887040/annotation2182502/range2182502/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K887040/annotation2182502/range2182502/1
type: Range
persistent identity: range2182502
display id: range2182502
version: 1
top level: BBa_K887040
direction: direction/reverse
start: 209
end: 220
orientation: inline

BBa_K323105
type: SequenceAnnotation
persistent identity: annotation2182503
display id: annotation2182503
version: 1
top level: BBa_K887040
location: https://synbiohub.org/public/igem/BBa_K887040/annotation2182503/range2182503/1
component: BBa_K323105

https://synbiohub.org/public/igem/BBa_K887040/annotation2182503/range2182503/1
type: Range
persistent identity: range2182503
display id: range2182503
version: 1
top level: BBa_K887040
direction: direction/reverse
start: 229
end: 522
orientation: inline

BBa_K539713
type: SequenceAnnotation
persistent identity: annotation2182505
display id: annotation2182505
version: 1
top level: BBa_K887040
location: https://synbiohub.org/public/igem/BBa_K887040/annotation2182505/range2182505/1
component: BBa_K539713

https://synbiohub.org/public/igem/BBa_K887040/annotation2182505/range2182505/1
type: Range
persistent identity: range2182505
display id: range2182505
version: 1
top level: BBa_K887040
direction: direction/reverse
start: 529
end: 2244
orientation: inline

BBa_K887020
type: ComponentDefinition
persistent identity: BBa K887020
display id: BBa_K887020
version: 1
was generated by: iGEM to SBOL conversion
description: PBSII (zinc finger) + ilvC (enzyme)
created: 2012-09-08T11:00:00Z
modified: 2015-05-08T01:13:40Z
mutable provenance: =D
owned by: james
owned by: myers
mutable description: It's a combination of the zinc finger (PBSII) and the enzyme (ilvC) that can make the isobutanol pathway more efficient.
discontinued: false
dominant: false
experience: experience/None
group u list: _1150_
m user id: 0
owner id: 14223
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: =D
star: false
creator: Tzu-Yun Hsu
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K539711
component: BBa_K323107
sequence annotation: BBa_K323107
sequence annotation: BBa_K539711
sequence: https://synbiohub.org/public/igem/BBa_K887020_sequence/1

BBa_K539711
type: Component
persistent identity: component2182359
display id: component2182359
version: 1
top level: BBa_K887020
definition: BBa_K539711
access: public

BBa_K323107
type: Component
persistent identity: component2182357
display id: component2182357
version: 1
top level: BBa_K887020
definition: BBa_K323107
access: public

BBa_K323107
type: SequenceAnnotation
persistent identity: annotation2182357
display id: annotation2182357
version: 1
top level: BBa_K887020
location: https://synbiohub.org/public/igem/BBa_K887020/annotation2182357/range2182357/1
component: BBa_K323107

https://synbiohub.org/public/igem/BBa_K887020/annotation2182357/range2182357/1
type: Range
persistent identity: range2182357
display id: range2182357
version: 1
top level: BBa_K887020
direction: direction/reverse
start: 1
end: 288
orientation: inline

BBa_K539711
type: SequenceAnnotation
persistent identity: annotation2182359
display id: annotation2182359
version: 1
top level: BBa_K887020
location: https://synbiohub.org/public/igem/BBa_K887020/annotation2182359/range2182359/1
component: BBa_K539711

https://synbiohub.org/public/igem/BBa_K887020/annotation2182359/range2182359/1
type: Range
persistent identity: range2182359
display id: range2182359
version: 1
top level: BBa_K887020
direction: direction/reverse
start: 295
end: 1770
orientation: inline

BBa_K887023
type: ComponentDefinition
persistent identity: BBa K887023
display id: BBa_K887023
version: 1
was generated by: iGEM to SBOL conversion
description: HivC+ilvD
created: 2012-09-12T11:00:00Z
modified: 2015-05-08T01:13:40Z
mutable provenance: 123
owned by: james
owned by: myers
mutable description: This is a part with HivC, which is a zinc finger binding domain, and combined with ilvD, which is the third enzyme in the isobutanol synthesis pathway. By this way, we can rearrange this fusion protein in order on the DNA program, which include 5'GAT GCT GCA'3 sequence, and we treat others enzyme in isobutanol synthesis pathway by the same method, we can make this reaction be more efficient. As a result, we can get higher yield easily.
discontinued: true
dominant: false
experience: experience/None
group u list: _1150_
m user id: 0
owner id: 14160
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: 123
star: false
creator: PENG, KAI-CHUN
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K539712
component: BBa_K165006
sequence annotation: BBa_K165006
sequence annotation: BBa_K539712
sequence: https://synbiohub.org/public/igem/BBa_K887023_sequence/1

BBa_K539712
type: Component
persistent identity: component2182779
display id: component2182779
version: 1
top level: BBa_K887023
definition: BBa_K539712
access: public

BBa_K165006
type: Component
persistent identity: component2182777
display id: component2182777
version: 1
top level: BBa_K887023
definition: BBa_K165006
access: public

BBa_K165006
type: SequenceAnnotation
persistent identity: annotation2182777
display id: annotation2182777
version: 1
top level: BBa_K887023
location: https://synbiohub.org/public/igem/BBa_K887023/annotation2182777/range2182777/1
component: BBa_K165006

https://synbiohub.org/public/igem/BBa_K887023/annotation2182777/range2182777/1
type: Range
persistent identity: range2182777
display id: range2182777
version: 1
top level: BBa_K887023
direction: direction/reverse
start: 1
end: 252
orientation: inline

BBa_K539712
type: SequenceAnnotation
persistent identity: annotation2182779
display id: annotation2182779
version: 1
top level: BBa_K887023
location: https://synbiohub.org/public/igem/BBa_K887023/annotation2182779/range2182779/1
component: BBa_K539712

https://synbiohub.org/public/igem/BBa_K887023/annotation2182779/range2182779/1
type: Range
persistent identity: range2182779
display id: range2182779
version: 1
top level: BBa_K887023
direction: direction/reverse
start: 259
end: 2109
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_K165006
type: ComponentDefinition
persistent identity: BBa K165006
display id: BBa_K165006
version: 1
was generated by: iGEM to SBOL conversion
description: Zif268-HIV DNA-binding domain
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: -
star: false
creator: John Szymanski
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K165006_sequence/1

BBa_K323107
type: ComponentDefinition
persistent identity: BBa K323107
display id: BBa_K323107
version: 1
was generated by: iGEM to SBOL conversion
description: PBSII zinc finger + linker
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:01Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _443_
m user id: 0
owner id: 7571
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Tina Lebar
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K323107_sequence/1

BBa_K323105
type: ComponentDefinition
persistent identity: BBa K323105
display id: BBa_K323105
version: 1
was generated by: iGEM to SBOL conversion
description: Zif268 zinc finger + linker
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:01Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _443_
m user id: 0
owner id: 7571
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Tina Lebar
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K323105_sequence/1

BBa_K539711
type: ComponentDefinition
persistent identity: BBa K539711
display id: BBa_K539711
version: 1
was generated by: iGEM to SBOL conversion
description: ilvC (ketol-acid reductoisomerase NAD(P)-binding)
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: It comes from Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
mutable description: This is the coding region of ilvC from Escherichia coli str. K-12 substr(MG1655). It encodes ketol-acid reductoisomerase NAD(P)-binding which is part of the isobutanol biosynthesis pathway.
discontinued: false
dominant: false
experience: experience/None
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding gene, we have to delete those RE site in kivd. The RE site in the part has been modified by point mutation.
star: false
creator: Yang, Yi-Pei
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ilvC coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K539711_sequence/1

ilvC coding sequence
type: SequenceAnnotation
persistent identity: annotation2136206
display id: annotation2136206
version: 1
top level: BBa_K539711
location: https://synbiohub.org/public/igem/BBa_K539711/annotation2136206/range2136206/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K539711/annotation2136206/range2136206/1
type: Range
persistent identity: range2136206
display id: range2136206
version: 1
top level: BBa_K539711
direction: direction/forward
start: 1
end: 1476
orientation: inline

BBa_K539712
type: ComponentDefinition
persistent identity: BBa K539712
display id: BBa_K539712
version: 1
was generated by: iGEM to SBOL conversion
description: ilvD (dihydroxyacid dehydratase)
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: It comes from Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
mutable description: This is the coding sequence of ilvD form Escherichia coli str. K-12 substr. MG1655. It encodes dihydroxyacid dehydratase which is part of isobutanol biosynthesis pathway.
discontinued: false
dominant: false
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in coding sequence. There is one Pst1 in original sequence of ilvD. However, the RE site in ilvD has been modified by point mutation.
star: false
creator: Yang, Yi-Pei
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ilvD
sequence: https://synbiohub.org/public/igem/BBa_K539712_sequence/1

ilvD
type: SequenceAnnotation
persistent identity: annotation2136209
display id: annotation2136209
version: 1
top level: BBa_K539712
location: https://synbiohub.org/public/igem/BBa_K539712/annotation2136209/range2136209/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K539712/annotation2136209/range2136209/1
type: Range
persistent identity: range2136209
display id: range2136209
version: 1
top level: BBa_K539712
direction: direction/forward
start: 1
end: 1851
orientation: inline

BBa_K539713
type: ComponentDefinition
persistent identity: BBa K539713
display id: BBa_K539713
version: 1
was generated by: iGEM to SBOL conversion
description: AlsS (alpha-acetolactate synthase protein)
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: It comes from Bacillus subtilis.
owned by: james
owned by: myers
mutable description: This is the coding region of AlsS from Bacillus subtilis. It encodes alpha-acetolactate synthase protein which is part of isobutanol biosynthesis pathway.
discontinued: false
dominant: false
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in coding sequence. There are two Pst1 site, one Xba1 site and one EcoR1 site in original sequence of AlsS. However, those RE site in AlsS has been modified by point mutation.
star: false
creator: Yang, Yi-Pei
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AlsS
sequence: https://synbiohub.org/public/igem/BBa_K539713_sequence/1

AlsS
type: SequenceAnnotation
persistent identity: annotation2136210
display id: annotation2136210
version: 1
top level: BBa_K539713
location: https://synbiohub.org/public/igem/BBa_K539713/annotation2136210/range2136210/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K539713/annotation2136210/range2136210/1
type: Range
persistent identity: range2136210
display id: range2136210
version: 1
top level: BBa_K539713
direction: direction/forward
start: 1
end: 1716
orientation: inline

https://synbiohub.org/public/igem/BBa_K887072_sequence/1
type: Sequence
persistent identity: BBa K887072 sequence
display id: BBa_K887072_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagccgggtgaaaaaccgtatgcatgtccggttgaaagctgtgatcgtcgttttagccgtagtgatgaactgacccgtcatattcgtattcatacaggtcagaaaccgtttcagtgtcgtatttgcatgcgtaattttagccgttcagatcatctgaccacccatattcgtacccatactggcgaaaaaccgtttgcctgtgatatttgtggtcgtaaatttgcacgttccgatgaacgtaaacgccataccaaaattcatacgggtggtggtagcggtggtggttcaggtggtagttactagatgttgacaaaagcaacaaaagaacaaaaatcccttgtgaaaaacagaggggcggagcttgttgttgattgcttagtggagcaaggtgtcacacatgtatttggcattccaggtgcaaaaattgatgcggtatttgacgctttacaagataaaggacctgaaattatcgttgcccggcacgaacaaaacgcagcattcatggcccaagcagtcggccgtttaactggaaaaccgggagtcgtgttagtcacatcaggaccgggtgcctctaacttggcaacaggcctgctgacagcgaacactgaaggagaccctgtcgttgcgcttgctggaaacgtgatccgtgcagatcgtttaaaacggacacatcaatctttggataatgcggcgctattccagccgattacaaaatacagtgtagaagttcaagatgtaaaaaatataccggaagctgttacaaatgcatttaggatagcgtcagcagggcaggctggggccgcttttgtgagctttccgcaagatgttgtgaatgaagtcacaaatacgaaaaacgtgcgtgctgttgcagcgccaaaactcggtccagcagcagatgatgcaatcagtgcggccatagcaaaaatccaaacagcaaaacttcctgtcgttttggtcggcatgaaaggcggaagaccggaagcaattaaggcggttcgcaagcttttgaaaaaggttcagcttccatttgttgaaacatatcaagctgccggtaccctttccagagatttagaggatcaatattttggccgtatcggtttgttccgcaaccagcctggcgatttactgctagagcaggcagatgttgttctgacgatcggctatgacccgattgaatatgatccgaaattctggaatatcaatggagaccggacaattatccatttagacgagattatcgctgacattgatcatgcttaccagcctgatcttgaattgatcggtgacattccgtccacgatcaatcatatcgaacacgatgctgtgaaagtggaatttgcagagcgtgagcagaaaatcctttctgatttaaaacaatatatgcatgaaggtgagcaggtgccagcagattggaaatcagacagagcgcaccctcttgaaatcgttaaagagttgcgtaatgcagtcgatgatcatgttacagtaacttgcgatatcggttcgcacgccatttggatgtcacgttatttccgcagctacgagccgttaacattaatgatcagtaacggtatgcaaacactcggcgttgcgcttccttgggcaatcggcgcttcattggtgaaaccgggagaaaaagtggtttctgtctctggtgacggcggtttcttattctcagcaatggaattagagacagcagttcgactaaaagcaccaattgtacacattgtatggaacgacagcacatatgacatggttgcattccagcaattgaaaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K887040_sequence/1
type: Sequence
persistent identity: BBa K887040 sequence
display id: BBa_K887040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagccgggtgaaaaaccgtatgcatgtccggttgaaagctgtgatcgtcgttttagccgtagtgatgaactgacccgtcatattcgtattcatacaggtcagaaaccgtttcagtgtcgtatttgcatgcgtaattttagccgttcagatcatctgaccacccatattcgtacccatactggcgaaaaaccgtttgcctgtgatatttgtggtcgtaaatttgcacgttccgatgaacgtaaacgccataccaaaattcatacgggtggtggtagcggtggtggttcaggtggtagttactagatgttgacaaaagcaacaaaagaacaaaaatcccttgtgaaaaacagaggggcggagcttgttgttgattgcttagtggagcaaggtgtcacacatgtatttggcattccaggtgcaaaaattgatgcggtatttgacgctttacaagataaaggacctgaaattatcgttgcccggcacgaacaaaacgcagcattcatggcccaagcagtcggccgtttaactggaaaaccgggagtcgtgttagtcacatcaggaccgggtgcctctaacttggcaacaggcctgctgacagcgaacactgaaggagaccctgtcgttgcgcttgctggaaacgtgatccgtgcagatcgtttaaaacggacacatcaatctttggataatgcggcgctattccagccgattacaaaatacagtgtagaagttcaagatgtaaaaaatataccggaagctgttacaaatgcatttaggatagcgtcagcagggcaggctggggccgcttttgtgagctttccgcaagatgttgtgaatgaagtcacaaatacgaaaaacgtgcgtgctgttgcagcgccaaaactcggtccagcagcagatgatgcaatcagtgcggccatagcaaaaatccaaacagcaaaacttcctgtcgttttggtcggcatgaaaggcggaagaccggaagcaattaaggcggttcgcaagcttttgaaaaaggttcagcttccatttgttgaaacatatcaagctgccggtaccctttccagagatttagaggatcaatattttggccgtatcggtttgttccgcaaccagcctggcgatttactgctagagcaggcagatgttgttctgacgatcggctatgacccgattgaatatgatccgaaattctggaatatcaatggagaccggacaattatccatttagacgagattatcgctgacattgatcatgcttaccagcctgatcttgaattgatcggtgacattccgtccacgatcaatcatatcgaacacgatgctgtgaaagtggaatttgcagagcgtgagcagaaaatcctttctgatttaaaacaatatatgcatgaaggtgagcaggtgccagcagattggaaatcagacagagcgcaccctcttgaaatcgttaaagagttgcgtaatgcagtcgatgatcatgttacagtaacttgcgatatcggttcgcacgccatttggatgtcacgttatttccgcagctacgagccgttaacattaatgatcagtaacggtatgcaaacactcggcgttgcgcttccttgggcaatcggcgcttcattggtgaaaccgggagaaaaagtggtttctgtctctggtgacggcggtttcttattctcagcaatggaattagagacagcagttcgactaaaagcaccaattgtacacattgtatggaacgacagcacatatgacatggttgcattccagcaattgaaaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K887020_sequence/1
type: Sequence
persistent identity: BBa K887020 sequence
display id: BBa_K887020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccgggtgaaaaaccgtatgcatgtcctgaatgtggtaaaagctttagccagcgtgcaaatctgcgtgcacatcagcgtacccatacaggtgaaaagccttataaatgcccagaatgcggcaaaagcttttcacgtagcgatcatctgaccacccatcagcgcacacatactggcgagaaaccttacaaatgtccagagtgtggtaaatcatttagccgtagtgatgttctggttcgtcatcagagaacccacacgggtggtggtagcggtggtggttcaggtggtagttactagatggctaactacttcaatacactgaatctgcgccagcagctggcacagctgggcaaatgtcgctttatgggccgcgatgaatttgccgatggcgcgagctaccttcagggtaaaaaagtagtcatcgtcggctgtggcgcacagggtctgaaccagggcctgaacatgcgtgattctggtctcgatatctcctacgctctgcgtaaagaagcgattgccgagaagcgcgcgtcctggcgtaaagcgaccgaaaatggttttaaagtgggtacttacgaagaactgatcccacaggcggatctggtgattaacctgacgccggacaagcagcactctgatgtagtgcgcaccgtacagccactgatgaaagacggcgcggcgctgggctactcgcacggtttcaacatcgtcgaagtgggcgagcagatccgtaaagatatcaccgtagtgatggttgcgccgaaatgcccaggcaccgaagtgcgtgaagagtacaaacgtgggttcggcgtaccgacgctgattgccgttcacccggaaaacgatccgaaaggcgaaggcatggcgattgccaaagcctgggcggctgcaaccggtggtcaccgtgcgggtgtgctggaatcgtccttcgttgcggaagtgaaatctgatctgatgggcgagcaaaccatcctgtgcggtatgttgcaggctggctctctgctgtgcttcgacaagctggtggaagaaggtaccgatccagcatacgcagaaaaactgattcagttcggttgggaaaccatcaccgaagcactgaaacagggcggcatcaccctgatgatggaccgtctctctaacccggcgaaactgcgtgcttatgcgctttctgaacagctgaaagagatcatggcacccctgttccagaaacatatggacgacatcatctccggcgaattttcttccggtatgatggcggactgggccaacgatgataagaaactgctgacctggcgtgaagagaccggcaaaaccgcgtttgaaaccgcgccgcagtatgaaggcaaaatcggcgagcaggagtacttcgataaaggcgtactgatgattgcgatggtgaaagcgggcgttgaactggcgttcgaaaccatggtcgattccggcatcattgaagagtctgcatattatgaatcactgcacgagctgccgctgattgccaacaccatcgcccgtaagcgtctgtacgaaatgaacgtggttatctctgataccgctgagtacggtaactatctgttctcttacgcttgtgtgccgttgctgaaaccgtttatggcagagctgcaaccgggcgacctgggtaaagctattccggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K887023_sequence/1
type: Sequence
persistent identity: BBa K887023 sequence
display id: BBa_K887023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaatactagatgcctaagtaccgttccgccaccaccactcatggtcgtaatatggcgggtgctcgtgcgctgtggcgcgccaccggaatgaccgacgccgatttcggtaagccgattatcgcggttgtgaactcgttcacccaatttgtaccgggtcacgtccatctgcgcgatctcggtaaactggtcgccgaacaaattgaagcggctggcggcgttgccaaagagttcaacaccattgcggtggatgatgggattgccatgggccacggggggatgctttattcactgccatctcgcgaactgatcgctgattccgttgagtatatggtcaacgcccactgcgccgacgccatggtctgcatctctaactgcgacaaaatcaccccggggatgctgatggcttccctgcgcctgaatattccggtgatctttgtttccggcggcccgatggaggccgggaaaaccaaactttccgatcagatcatcaagctcgatctggtcgatgcgatgatccagggcgcagacccgaaagtatctgactcccagagcgatcaggttgaacgttccgcgtgtccgacctgcggttcctgctccgggatgtttaccgctaactcaatgaactgcctgaccgaagcgctgggcctgtcgcagccgggcaacggctcgctgctggcaacccacgccgaccgtaagcagctgttccttaatgctggtaaacgcattgttgaattgaccaaacgttattacgagcaaaacgacgaaagtgcactgccgcgtaatatcgccagtaaggcggcgtttgaaaacgccatgacgctggatatcgcgatgggtggatcgactaacaccgtacttcacctgctggcggcggcgcaggaagcggaaatcgacttcaccatgagtgatatcgataagctttcccgcaaggttccacagctgtgtaaagttgcgccgagcacccagaaataccatatggaagatgttcaccgtgctggtggtgttatcggtattctcggcgaactggatcgcgcggggttactgaaccgtgatgtgaaaaacgtacttggcctgacgttgccgcaaacgctggaacaatacgacgttatgctgacccaggatgacgcggtaaaaaatatgttccgcgcaggtccagcaggcattcgtaccacacaggcattctcgcaagattgccgttgggatacgctggacgacgatcgcgccaatggctgtatccgctcgctggaacacgcctacagcaaagacggcggcctggcggtgctctacggtaactttgcggaaaacggctgcatcgtgaaaacggcaggcgtcgatgacagcatcctcaaattcaccggcccggcgaaagtgtacgaaagccaggacgatgcggtagaagcgattctcggcggtaaagttgtcgccggagatgtggtagtaattcgctatgaaggcccgaaaggcggtccggggatgcaggaaatgctctacccaaccagcttcctgaaatcaatgggtctcggcaaagcctgtgcgctgatcaccgacggtcgtttctctggtggcacctctggtctttccatcggccacgtctcaccggaagcggcaagcggcggcagcattggcctgattgaagatggtgacctgatcgctatcgacatcccgaaccgtggcattcagttacaggtaagcgatgccgaactggcggcgcgtcgtgaagcgcaggacgctcgaggtgacaaagcctggac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165006_sequence/1
type: Sequence
persistent identity: BBa K165006 sequence
display id: BBa_K165006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K323107_sequence/1
type: Sequence
persistent identity: BBa K323107 sequence
display id: BBa_K323107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccgggtgaaaaaccgtatgcatgtcctgaatgtggtaaaagctttagccagcgtgcaaatctgcgtgcacatcagcgtacccatacaggtgaaaagccttataaatgcccagaatgcggcaaaagcttttcacgtagcgatcatctgaccacccatcagcgcacacatactggcgagaaaccttacaaatgtccagagtgtggtaaatcatttagccgtagtgatgttctggttcgtcatcagagaacccacacgggtggtggtagcggtggtggttcaggtggtagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K323105_sequence/1
type: Sequence
persistent identity: BBa K323105 sequence
display id: BBa_K323105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccgggtgaaaaaccgtatgcatgtccggttgaaagctgtgatcgtcgttttagccgtagtgatgaactgacccgtcatattcgtattcatacaggtcagaaaccgtttcagtgtcgtatttgcatgcgtaattttagccgttcagatcatctgaccacccatattcgtacccatactggcgaaaaaccgtttgcctgtgatatttgtggtcgtaaatttgcacgttccgatgaacgtaaacgccataccaaaattcatacgggtggtggtagcggtggtggttcaggtggtagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539711_sequence/1
type: Sequence
persistent identity: BBa K539711 sequence
display id: BBa_K539711_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctaactacttcaatacactgaatctgcgccagcagctggcacagctgggcaaatgtcgctttatgggccgcgatgaatttgccgatggcgcgagctaccttcagggtaaaaaagtagtcatcgtcggctgtggcgcacagggtctgaaccagggcctgaacatgcgtgattctggtctcgatatctcctacgctctgcgtaaagaagcgattgccgagaagcgcgcgtcctggcgtaaagcgaccgaaaatggttttaaagtgggtacttacgaagaactgatcccacaggcggatctggtgattaacctgacgccggacaagcagcactctgatgtagtgcgcaccgtacagccactgatgaaagacggcgcggcgctgggctactcgcacggtttcaacatcgtcgaagtgggcgagcagatccgtaaagatatcaccgtagtgatggttgcgccgaaatgcccaggcaccgaagtgcgtgaagagtacaaacgtgggttcggcgtaccgacgctgattgccgttcacccggaaaacgatccgaaaggcgaaggcatggcgattgccaaagcctgggcggctgcaaccggtggtcaccgtgcgggtgtgctggaatcgtccttcgttgcggaagtgaaatctgatctgatgggcgagcaaaccatcctgtgcggtatgttgcaggctggctctctgctgtgcttcgacaagctggtggaagaaggtaccgatccagcatacgcagaaaaactgattcagttcggttgggaaaccatcaccgaagcactgaaacagggcggcatcaccctgatgatggaccgtctctctaacccggcgaaactgcgtgcttatgcgctttctgaacagctgaaagagatcatggcacccctgttccagaaacatatggacgacatcatctccggcgaattttcttccggtatgatggcggactgggccaacgatgataagaaactgctgacctggcgtgaagagaccggcaaaaccgcgtttgaaaccgcgccgcagtatgaaggcaaaatcggcgagcaggagtacttcgataaaggcgtactgatgattgcgatggtgaaagcgggcgttgaactggcgttcgaaaccatggtcgattccggcatcattgaagagtctgcatattatgaatcactgcacgagctgccgctgattgccaacaccatcgcccgtaagcgtctgtacgaaatgaacgtggttatctctgataccgctgagtacggtaactatctgttctcttacgcttgtgtgccgttgctgaaaccgtttatggcagagctgcaaccgggcgacctgggtaaagctattccggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539712_sequence/1
type: Sequence
persistent identity: BBa K539712 sequence
display id: BBa_K539712_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcctaagtaccgttccgccaccaccactcatggtcgtaatatggcgggtgctcgtgcgctgtggcgcgccaccggaatgaccgacgccgatttcggtaagccgattatcgcggttgtgaactcgttcacccaatttgtaccgggtcacgtccatctgcgcgatctcggtaaactggtcgccgaacaaattgaagcggctggcggcgttgccaaagagttcaacaccattgcggtggatgatgggattgccatgggccacggggggatgctttattcactgccatctcgcgaactgatcgctgattccgttgagtatatggtcaacgcccactgcgccgacgccatggtctgcatctctaactgcgacaaaatcaccccggggatgctgatggcttccctgcgcctgaatattccggtgatctttgtttccggcggcccgatggaggccgggaaaaccaaactttccgatcagatcatcaagctcgatctggtcgatgcgatgatccagggcgcagacccgaaagtatctgactcccagagcgatcaggttgaacgttccgcgtgtccgacctgcggttcctgctccgggatgtttaccgctaactcaatgaactgcctgaccgaagcgctgggcctgtcgcagccgggcaacggctcgctgctggcaacccacgccgaccgtaagcagctgttccttaatgctggtaaacgcattgttgaattgaccaaacgttattacgagcaaaacgacgaaagtgcactgccgcgtaatatcgccagtaaggcggcgtttgaaaacgccatgacgctggatatcgcgatgggtggatcgactaacaccgtacttcacctgctggcggcggcgcaggaagcggaaatcgacttcaccatgagtgatatcgataagctttcccgcaaggttccacagctgtgtaaagttgcgccgagcacccagaaataccatatggaagatgttcaccgtgctggtggtgttatcggtattctcggcgaactggatcgcgcggggttactgaaccgtgatgtgaaaaacgtacttggcctgacgttgccgcaaacgctggaacaatacgacgttatgctgacccaggatgacgcggtaaaaaatatgttccgcgcaggtccagcaggcattcgtaccacacaggcattctcgcaagattgccgttgggatacgctggacgacgatcgcgccaatggctgtatccgctcgctggaacacgcctacagcaaagacggcggcctggcggtgctctacggtaactttgcggaaaacggctgcatcgtgaaaacggcaggcgtcgatgacagcatcctcaaattcaccggcccggcgaaagtgtacgaaagccaggacgatgcggtagaagcgattctcggcggtaaagttgtcgccggagatgtggtagtaattcgctatgaaggcccgaaaggcggtccggggatgcaggaaatgctctacccaaccagcttcctgaaatcaatgggtctcggcaaagcctgtgcgctgatcaccgacggtcgtttctctggtggcacctctggtctttccatcggccacgtctcaccggaagcggcaagcggcggcagcattggcctgattgaagatggtgacctgatcgctatcgacatcccgaaccgtggcattcagttacaggtaagcgatgccgaactggcggcgcgtcgtgaagcgcaggacgctcgaggtgacaaagcctggacgccgaaaaatcgtgaacgtcaggtctcctttgccctgcgtgcttatgccagcctggcaaccagcgccgacaaaggcgcggtgcgcgataaatcgaaactggggggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539713_sequence/1
type: Sequence
persistent identity: BBa K539713 sequence
display id: BBa_K539713_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttgacaaaagcaacaaaagaacaaaaatcccttgtgaaaaacagaggggcggagcttgttgttgattgcttagtggagcaaggtgtcacacatgtatttggcattccaggtgcaaaaattgatgcggtatttgacgctttacaagataaaggacctgaaattatcgttgcccggcacgaacaaaacgcagcattcatggcccaagcagtcggccgtttaactggaaaaccgggagtcgtgttagtcacatcaggaccgggtgcctctaacttggcaacaggcctgctgacagcgaacactgaaggagaccctgtcgttgcgcttgctggaaacgtgatccgtgcagatcgtttaaaacggacacatcaatctttggataatgcggcgctattccagccgattacaaaatacagtgtagaagttcaagatgtaaaaaatataccggaagctgttacaaatgcatttaggatagcgtcagcagggcaggctggggccgcttttgtgagctttccgcaagatgttgtgaatgaagtcacaaatacgaaaaacgtgcgtgctgttgcagcgccaaaactcggtccagcagcagatgatgcaatcagtgcggccatagcaaaaatccaaacagcaaaacttcctgtcgttttggtcggcatgaaaggcggaagaccggaagcaattaaggcggttcgcaagcttttgaaaaaggttcagcttccatttgttgaaacatatcaagctgccggtaccctttccagagatttagaggatcaatattttggccgtatcggtttgttccgcaaccagcctggcgatttactgctagagcaggcagatgttgttctgacgatcggctatgacccgattgaatatgatccgaaattctggaatatcaatggagaccggacaattatccatttagacgagattatcgctgacattgatcatgcttaccagcctgatcttgaattgatcggtgacattccgtccacgatcaatcatatcgaacacgatgctgtgaaagtggaatttgcagagcgtgagcagaaaatcctttctgatttaaaacaatatatgcatgaaggtgagcaggtgccagcagattggaaatcagacagagcgcaccctcttgaaatcgttaaagagttgcgtaatgcagtcgatgatcatgttacagtaacttgcgatatcggttcgcacgccatttggatgtcacgttatttccgcagctacgagccgttaacattaatgatcagtaacggtatgcaaacactcggcgttgcgcttccttgggcaatcggcgcttcattggtgaaaccgggagaaaaagtggtttctgtctctggtgacggcggtttcttattctcagcaatggaattagagacagcagttcgactaaaagcaccaattgtacacattgtatggaacgacagcacatatgacatggttgcattccagcaattgaaaaaatataaccgtacatctgcggtcgatttcggaaatatcgatatcgtgaaatatgcggaaagcttcggagcaactggcttgcgcgtagaatcaccagaccagctggcagatgttctgcgtcaaggcatgaacgctgaaggtcctgtcatcatcgatgtcccggttgactacagtgataacattaatttagcaagtgacaagcttccgaaagaatttggggaactcatgaaaacgaaagctctctag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z