BBa_K888001 1 BBa_K888001 AIDA-I translocator domain 2012-09-23T11:00:00Z 2015-05-08T01:13:40Z E. coli AIDA-I is synthetized as a 132 kDa pre-protein featuring a signal peptide which is cleaved during transport trough the inner membrane, a 78 kDa adhesin (passenger) domain, and a 45 kDa translocator. This autotransporter has a large capability in translocating relatively large passengers from 12-65 kDa by showing a N-terminal type of fusion (Li et al. 2007; van Bloois et al. 2011). Coupled with a passenger domain and a signal peptide (K888002), it is possible to express functional proteins in the outer membrane of E. coli van Bloois et al. (2011). Decorating microbes: surface display of proteins on Escherichia coli. Trends Biotechnol. 29(2): 79-86 Li et al. (2007). Presentation of Functional Organophosphorus Hydrolase Fusions on the Surface of Escherichia coli by the AIDA-I Autotransporter Pathway Biotechnol Bioeng. 99(2): 485-90 false false _1151_ 0 12987 9 Not in stock false Since the fusion of a passenger domain whit AIDA-I translocator domain, it is required a signal peptide for the passenger expression in the outer membrane false Juan Quijano annotation2196286 1 cds range2196286 1 1 1482 BBa_K888001_sequence 1 aacaacaatggttcaatcgttatcaacaacagtattatcaatggcaacatcacgaatgacgctgacctgagttttggcacggcgaagctgctgtctgcgacggtcaatggcagtctggtgaacaataaaaacattatcctgaatccgaccaaggaatccgccggtaacacgctgaccgtgtcaaattatacgggtaccccgggcagcgttatctctctgggcggtgtcctggaaggcgataactcgctgacggaccgtctggtggttaaaggtaataccagtggccaatccgatatcgtgtacgttaacgaagacggtagcggcggtcagacccgtgatggcattaacattatcagcgttgagggtaattctgacgcggaatttagtctgaaaaatcgcgtcgtggcaggcgcttatgattacaccctgcaaaagggtaacgaatctggcacggacaataagggttggtatctgacctcccatctgccgacgtcagatacccgtcagtatcgcccggaaaacggttcgtacgccaccaacatggcgctggccaatagcctgtttctgatggatctgaatgaacgtaaacaattccgcgcaatgagcgacaacacgcagccggaaagtgcttccgtgtggatgaaaattaccggcggtatcagctctggtaaactgaacgatggccagaataagaccacgaccaaccagttcatcaatcaactgggcggtgatatttataaatttcacgcggaacagctgggtgacttcaccctgggcatcatgggcggttatgcaaatgctaaaggcaagacgattaactacacctctaataaagcggcccgtaacacgctggatggttatagtgtcggtgtgtatggcacctggtaccaaaacggtgaaaatgcgacgggcctgtttgccgaaacctggatgcagtacaactggttcaatgcgagcgtcaaaggtgatggcctggaagaagaaaagtataacctgaatggtctgaccgcatccgccggcggtggctacaacctgaatgttcatacgtggacctcaccggaaggtattaccggcgaattttggctgcaaccgcatctgcaagcagtttggatgggcgtcacgccggatacccaccaggaagacaatggtacggttgtccaaggtgctggcaaaaacaatatccagaccaaggcaggcattcgtgcttcgtggaaagtgaagagcacgctggataaagacaccggtcgtcgctttcgcccgtatatcgaagcaaactggattcataatacccacgaatttggtgtgaaaatgtcagatgactcgcaactgctgtcaggttcgcgcaaccagggcgaaattaagacgggtatcgaaggcgttattacccaaaacctgagtgtgaatggtggcgttgcctaccaggcaggcggtcacggttctaacgcaatctctggtgctctgggcatcaaatactccttctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z