BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_K888003
1
BBa_K888003
GlpF (BBa_K190028) coding sequence with constitutive promoter and RBS (K081005)
2012-09-24T11:00:00Z
2015-05-08T01:13:40Z
Both, the promoter and the RBS come from the part Bba_K081005 from the iGEM08_UNIPV-Pavia.
GflP coding sequence with cloned with the constitutive promoter Bba_J23100 and the strong RBS Bba_B0030.
false
false
_1151_
0
8594
9
It's complicated
false
Design by J. Quijano and constructed by R. Robles-Gonzalez
false
J. Quijano
component2198643
1
BBa_K081005
component2198646
1
BBa_K190028
annotation2198643
1
BBa_K081005
range2198643
1
1
58
annotation2198646
1
BBa_K190028
range2198646
1
65
910
BBa_K190028
1
BBa_K190028
GlpF
2009-08-25T11:00:00Z
2015-05-08T01:11:15Z
This part comes from genomic DNA of the Escherichia coli 536.
Released HQ 2013
GlpF is an aquaglyceroporin channel that facilitates the transport of As(III).
false
false
_306_
0
4226
9
In stock
true
removal of an EcoR1 restiction site
false
Jolanda Witteveen
annotation2018038
1
start
range2018038
1
1
3
annotation2061212
1
GlpF
range2061212
1
1
846
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K081005
1
BBa_K081005
constitutive promoter family member and RBS
2008-10-17T11:00:00Z
2015-05-08T01:08:34Z
Promoter: John Anderson.
RBS: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Released HQ 2013
Constitutive promoter (strong) with RBS (strong, efficiency=0.3)
false
true
_227_
0
2583
9
In stock
true
We used BioBrick Standard Assembly.
true
Lorenzo Pasotti, Paolo Magni
component1981865
1
BBa_B0030
component1981863
1
BBa_J23100
annotation1981863
1
BBa_J23100
range1981863
1
1
35
annotation1981865
1
BBa_B0030
range1981865
1
44
58
BBa_K888003_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgagtcaaacatcaaccttgaaaggccagtgcattgctgaatttctcggtaccgggttgttgattttcttcggtgtgggttgcgttgcagcactaaaagtcgctggtgcgtcttttggtcagtgggaaatcagtgtcatttggggactgggggtggcaatggccatctacctgaccgcaggggtttccggcgcgcatcttaatcccgctgttaccattgcattgtggctgtttgcctgtttcgacaagcgcaaagttattccttttatcgtttcacaagttgccggcgctttctgtgctgcggctttagtttacgggctttactacaatttatttttcgacttcgagcagactcatcacattgttcgcggcagcgttgaaagtgttgatctggctggcactttctctacttaccctaatcctcatatcaattttgtgcaggctttcgcagttgagatggtgattaccgctattctgatggggctgatcctggcgttaacggacgatggcaacggtgtaccacgcggccctttggctcccttgctgattggtctactgattgcggtcattggcgcatctatgggcccattgacaggttttgccatgaacccagcgcgtgacttcggtccgaaagtctttgcctggctggcgggctggggcaatgtcgcctttaccggcggcagagacattccttacttcctggtgccgcttttcggccctatcgttggcgcgattgtaggtgcatttgcctaccgcaaactgattggtcgccatttgccttgcgatatctgtgttgtggaagaaaaggaaaccacaactccttcagaacaaaaagcttcgctgtaa
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_K081005_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K190028_sequence
1
atgagtcaaacatcaaccttgaaaggccagtgcattgctgaatttctcggtaccgggttgttgattttcttcggtgtgggttgcgttgcagcactaaaagtcgctggtgcgtcttttggtcagtgggaaatcagtgtcatttggggactgggggtggcaatggccatctacctgaccgcaggggtttccggcgcgcatcttaatcccgctgttaccattgcattgtggctgtttgcctgtttcgacaagcgcaaagttattccttttatcgtttcacaagttgccggcgctttctgtgctgcggctttagtttacgggctttactacaatttatttttcgacttcgagcagactcatcacattgttcgcggcagcgttgaaagtgttgatctggctggcactttctctacttaccctaatcctcatatcaattttgtgcaggctttcgcagttgagatggtgattaccgctattctgatggggctgatcctggcgttaacggacgatggcaacggtgtaccacgcggccctttggctcccttgctgattggtctactgattgcggtcattggcgcatctatgggcccattgacaggttttgccatgaacccagcgcgtgacttcggtccgaaagtctttgcctggctggcgggctggggcaatgtcgcctttaccggcggcagagacattccttacttcctggtgccgcttttcggccctatcgttggcgcgattgtaggtgcatttgcctaccgcaaactgattggtcgccatttgccttgcgatatctgtgttgtggaagaaaaggaaaccacaactccttcagaacaaaaagcttcgctgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z