LOCUS       BBa_K888008             2199 bp    DNA     linear       09-May-2024
DEFINITION  pArsR-RBS-ArsR (J33201) with RBS-fLuc (K325101)
ACCESSION   BBa_K888008
VERSION     BBa_K888008.1  
FEATURES             Location/Qualifiers
     misc_feature    36..54
                     /label=ArsR binding site
     promoter        57..62
                     /label=-35 sequence
     promoter        80..85
                     /label=-10 sequence
     RBS             108..112
                     /label=rbs
     CDS             119..472
                     /label=arsR
     misc_feature    1..518
                     /label=BBa_J33201
     CDS             544..2193
                     /label=EPIC luciferase (Photinus pyralis)
     RBS             527..543
                     /label=rbs
     misc_feature    2194..2199
                     /label=stop
     misc_feature    527..2199
                     /label=BBa_K325101
ORIGIN
        1 ccaactcaaa attcacacct attaccttcc tctgcactta cacattcgtt aagtcatata
       61 tgtttttgac ttatccgctt cgaagagaga cactacctgc aacaatcagg agcgcaatat
      121 gtcatttctg ttacccatcc aattgttcaa aattcttgct gatgaaaccc gtctgggcat
      181 cgttttactg ctcagcgaac tgggagagtt atgcgtctgc gatctctgca ctgctctcga
      241 ccagtcgcag cccaagatct cccgccacct ggcattgctg cgtgaaagcg ggctattgct
      301 ggaccgcaag caaggtaagt gggttcatta ccgcttatca ccgcatattc cagcatgggc
      361 ggcgaaaatt attgatgagg cctggcgatg tgaacaggaa aaggttcagg cgattgtccg
      421 caacctggct cgacaaaact gttccgggga cagtaagaac atttgcagtt aaaaatttag
      481 ctaaacacat atgaattttc agatgtgttt tatccgggta ctagagttaa ggaggtaaaa
      541 aaaatggagg acgcgaaaaa catcaagaaa ggcccagcac cgttctatcc gctggaagat
      601 ggtaccgccg gtgaacaatt gcacaaagcg atgaaacgtt atgcgctggt tccgggtacc
      661 atcgcattca cggacgcgca tatcgaggtt aacatcacct atgccgagta cttcgagatg
      721 agcgttcgtc tggccgaggc aatgaagcgt tatggtttga ataccaatca tcgcatcgtc
      781 gtctgtagcg agaacagctt gcagtttttc atgccagttc tgggtgcact gtttattggt
      841 gtggcagtgg cgcctgcgaa tgatatctac aacgagcgtg aactgttgaa tagcatgaac
      901 atttcgcagc cgacggttgt ttttgtgtcc aagaaaggtt tgcagaagat tctgaatgtg
      961 caaaagaagt tgccgatcat tcagaaaatc atcatcatgg acagcaagac cgattaccag
     1021 ggtttccaaa gcatgtacac ttttgtgacg agccacctgc ctccgggttt taacgagtat
     1081 gatttcgttc cggaaagctt cgaccgtgat aagacgattg cactgattat gaactcgtcc
     1141 ggctctaccg gcctgcctaa aggcgtggcc ctgccgcacc gtactgcatg tgtccgcttc
     1201 agccacgcgc gtgatccgat cttcggtaac caaatcattc cggacaccgc cattttgtct
     1261 gtcgttccgt ttcatcatgg ctttggtatg tttaccactc tgggttacct gatctgtggt
     1321 ttccgtgttg ttctgatgta ccgtttcgaa gaagaactgt tcctgcgtag cctgcaagat
     1381 tacaagatcc aatccgcgct gctggtgccg acgctgttta gcttcttcgc taaatccacc
     1441 ctgattgaca aatatgactt gagcaacctg cacgaaatcg cgtcgggcgg tgcgccgctg
     1501 agcaaagaag tcggtgaggc cgttgcgaaa cgtttccatc tgccgggcat ccgtcagggt
     1561 tatggcctga cggaaacgac cagcgcgatt ttgattaccc ctgagggtga cgataaaccg
     1621 ggtgccgttg gcaaggtggt accgttcttt gaggctaaag tcgtggacct ggacacgggt
     1681 aagactctgg gtgtgaatca acgtggcgag ttgtgcgttc gtggcccgat gatcatgagc
     1741 ggttatgtca acaacccgga agcaactaat gcgttgatcg ataaggatgg ctggctgcac
     1801 agcggcgacc tggcgtactg ggatgaggat gaacactttt tcatcgttgg tcgcctgaag
     1861 agcctgatca agtacaaggg ctaccaggtg gcaccggctg aactggagag cattctgttg
     1921 caacacccta atatcttcga cgcgggtgtt gcgggtctgc ctgatgatga tgccggtgaa
     1981 ttgccagctg cggtggtcgt tctggaacac ggtaaaacga tgactgaaaa agaaatcgtg
     2041 gactacgtcg cctcgcaggt gaccaccgct aaaaagttgc gtggtggcgt ggtgtttgtg
     2101 gatgaagtgc cgaaaggcct gacgggtaaa cgtgatgcac gtaagattcg tgagatcttg
     2161 atcaaggcga agaaaggtgg taaatccaag ttgtaataa
//