BBa_K325101
1
BBa_K325101
EPIC Firefly Luciferase <BR><i>Photinus Pyralis<BR>(E. coli optimised)</i>
2010-10-22T11:00:00Z
2015-05-08T01:12:02Z
a
a
false
false
_446_
0
7466
9
In stock
false
a
false
Ben Reeve and Hannah Copley
annotation2097944
1
stop
range2097944
1
1668
1673
annotation2097938
1
EPIC luciferase (Photinus pyralis)
range2097938
1
18
1667
annotation2097943
1
rbs
range2097943
1
1
17
BBa_J33201
1
BBa_J33201
E. coli chromosomal ars promoter with arsR repressor gene
2006-10-12T11:00:00Z
2015-08-31T04:08:46Z
The DNA was derived by PCR using genomic DNA from E. coli JM109. Primers were designed based on the sequence reported by Diorio et al (1995), J. Bacteriol 177 (8), 2050-2056, Genbank accession X80057, GI:510824. The sequence given here is derived by sequencing the biobrick construct.
Released HQ 2013
This part consists of the promoter of the E. coli JM109 chromosomal arsenic detoxification operon (ars operon), including the ArsR repressor binding site and the arsR gene encoding the arsR repressor protein, together with its ribosome binding site. Addition of any other genes to the 3' end of this part will result in their expression being dependent on the presence of sodium arsenate or sodium arsenite. Arsenite or arsenite anion binds to the repressor protein ArsR, resulting in inability to repress the promoter. Based on our experiments, a concentration of 1 micromolar sodium arsenate in LB is sufficient for essentially full expression, though this will vary according to conditions.
false
true
_63_
0
837
63
In stock
true
Note that this sequence includes the arsR gene encoding the ArsR repressor protein, which is thus negatively autoregulated. No additional parts are required for arsenate-induced expression from this part. In principle, this part should also function in hosts other than E. coli.
false
Chris French
annotation1902815
1
rbs
range1902815
1
108
112
annotation1902812
1
ArsR binding site
range1902812
1
36
54
annotation1902816
1
arsR
range1902816
1
119
472
annotation1902813
1
-35 sequence
range1902813
1
57
62
annotation1902814
1
-10 sequence
range1902814
1
80
85
BBa_K888008
1
BBa_K888008
pArsR-RBS-ArsR (J33201) with RBS-fLuc (K325101)
2012-09-24T11:00:00Z
2015-05-08T01:13:40Z
The pArsR-RBS-ArsR comes from the iGEM2006_Edinburgh team, and the part of RBS-fLuc comes from iGEM10_Cambridge.
This is the main part of the biosensor, it includes the regulatory part, RBS and the Ars gene from the arsenic detoxification operon (Ars operon), this part also includes a mutant of the luciferase from the North American firefly (P. pyralis), described as 10 times higher substrate affinity and luminescence output compared to wildtype.
false
false
_1151_
0
13095
9
Not in stock
false
Designed by J.F. Quijano
false
J.F. Quijano
component2198665
1
BBa_J33201
component2198669
1
BBa_K325101
annotation2198665
1
BBa_J33201
range2198665
1
1
518
annotation2198669
1
BBa_K325101
range2198669
1
527
2199
BBa_J33201_sequence
1
ccaactcaaaattcacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagacactacctgcaacaatcaggagcgcaatatgtcatttctgttacccatccaattgttcaaaattcttgctgatgaaacccgtctgggcatcgttttactgctcagcgaactgggagagttatgcgtctgcgatctctgcactgctctcgaccagtcgcagcccaagatctcccgccacctggcattgctgcgtgaaagcgggctattgctggaccgcaagcaaggtaagtgggttcattaccgcttatcaccgcatattccagcatgggcggcgaaaattattgatgaggcctggcgatgtgaacaggaaaaggttcaggcgattgtccgcaacctggctcgacaaaactgttccggggacagtaagaacatttgcagttaaaaatttagctaaacacatatgaattttcagatgtgttttatccggg
BBa_K888008_sequence
1
ccaactcaaaattcacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagacactacctgcaacaatcaggagcgcaatatgtcatttctgttacccatccaattgttcaaaattcttgctgatgaaacccgtctgggcatcgttttactgctcagcgaactgggagagttatgcgtctgcgatctctgcactgctctcgaccagtcgcagcccaagatctcccgccacctggcattgctgcgtgaaagcgggctattgctggaccgcaagcaaggtaagtgggttcattaccgcttatcaccgcatattccagcatgggcggcgaaaattattgatgaggcctggcgatgtgaacaggaaaaggttcaggcgattgtccgcaacctggctcgacaaaactgttccggggacagtaagaacatttgcagttaaaaatttagctaaacacatatgaattttcagatgtgttttatccgggtactagagttaaggaggtaaaaaaaatggaggacgcgaaaaacatcaagaaaggcccagcaccgttctatccgctggaagatggtaccgccggtgaacaattgcacaaagcgatgaaacgttatgcgctggttccgggtaccatcgcattcacggacgcgcatatcgaggttaacatcacctatgccgagtacttcgagatgagcgttcgtctggccgaggcaatgaagcgttatggtttgaataccaatcatcgcatcgtcgtctgtagcgagaacagcttgcagtttttcatgccagttctgggtgcactgtttattggtgtggcagtggcgcctgcgaatgatatctacaacgagcgtgaactgttgaatagcatgaacatttcgcagccgacggttgtttttgtgtccaagaaaggtttgcagaagattctgaatgtgcaaaagaagttgccgatcattcagaaaatcatcatcatggacagcaagaccgattaccagggtttccaaagcatgtacacttttgtgacgagccacctgcctccgggttttaacgagtatgatttcgttccggaaagcttcgaccgtgataagacgattgcactgattatgaactcgtccggctctaccggcctgcctaaaggcgtggccctgccgcaccgtactgcatgtgtccgcttcagccacgcgcgtgatccgatcttcggtaaccaaatcattccggacaccgccattttgtctgtcgttccgtttcatcatggctttggtatgtttaccactctgggttacctgatctgtggtttccgtgttgttctgatgtaccgtttcgaagaagaactgttcctgcgtagcctgcaagattacaagatccaatccgcgctgctggtgccgacgctgtttagcttcttcgctaaatccaccctgattgacaaatatgacttgagcaacctgcacgaaatcgcgtcgggcggtgcgccgctgagcaaagaagtcggtgaggccgttgcgaaacgtttccatctgccgggcatccgtcagggttatggcctgacggaaacgaccagcgcgattttgattacccctgagggtgacgataaaccgggtgccgttggcaaggtggtaccgttctttgaggctaaagtcgtggacctggacacgggtaagactctgggtgtgaatcaacgtggcgagttgtgcgttcgtggcccgatgatcatgagcggttatgtcaacaacccggaagcaactaatgcgttgatcgataaggatggctggctgcacagcggcgacctggcgtactgggatgaggatgaacactttttcatcgttggtcgcctgaagagcctgatcaagtacaagggctaccaggtggcaccggctgaactggagagcattctgttgcaacaccctaatatcttcgacgcgggtgttgcgggtctgcctgatgatgatgccggtgaattgccagctgcggtggtcgttctggaacacggtaaaacgatgactgaaaaagaaatcgtggactacgtcgcctcgcaggtgaccaccgctaaaaagttgcgtggtggcgtggtgtttgtggatgaagtgccgaaaggcctgacgggtaaacgtgatgcacgtaagattcgtgagatcttgatcaaggcgaagaaaggtggtaaatccaagttgtaataa
BBa_K325101_sequence
1
ttaaggaggtaaaaaaaatggaggacgcgaaaaacatcaagaaaggcccagcaccgttctatccgctggaagatggtaccgccggtgaacaattgcacaaagcgatgaaacgttatgcgctggttccgggtaccatcgcattcacggacgcgcatatcgaggttaacatcacctatgccgagtacttcgagatgagcgttcgtctggccgaggcaatgaagcgttatggtttgaataccaatcatcgcatcgtcgtctgtagcgagaacagcttgcagtttttcatgccagttctgggtgcactgtttattggtgtggcagtggcgcctgcgaatgatatctacaacgagcgtgaactgttgaatagcatgaacatttcgcagccgacggttgtttttgtgtccaagaaaggtttgcagaagattctgaatgtgcaaaagaagttgccgatcattcagaaaatcatcatcatggacagcaagaccgattaccagggtttccaaagcatgtacacttttgtgacgagccacctgcctccgggttttaacgagtatgatttcgttccggaaagcttcgaccgtgataagacgattgcactgattatgaactcgtccggctctaccggcctgcctaaaggcgtggccctgccgcaccgtactgcatgtgtccgcttcagccacgcgcgtgatccgatcttcggtaaccaaatcattccggacaccgccattttgtctgtcgttccgtttcatcatggctttggtatgtttaccactctgggttacctgatctgtggtttccgtgttgttctgatgtaccgtttcgaagaagaactgttcctgcgtagcctgcaagattacaagatccaatccgcgctgctggtgccgacgctgtttagcttcttcgctaaatccaccctgattgacaaatatgacttgagcaacctgcacgaaatcgcgtcgggcggtgcgccgctgagcaaagaagtcggtgaggccgttgcgaaacgtttccatctgccgggcatccgtcagggttatggcctgacggaaacgaccagcgcgattttgattacccctgagggtgacgataaaccgggtgccgttggcaaggtggtaccgttctttgaggctaaagtcgtggacctggacacgggtaagactctgggtgtgaatcaacgtggcgagttgtgcgttcgtggcccgatgatcatgagcggttatgtcaacaacccggaagcaactaatgcgttgatcgataaggatggctggctgcacagcggcgacctggcgtactgggatgaggatgaacactttttcatcgttggtcgcctgaagagcctgatcaagtacaagggctaccaggtggcaccggctgaactggagagcattctgttgcaacaccctaatatcttcgacgcgggtgttgcgggtctgcctgatgatgatgccggtgaattgccagctgcggtggtcgttctggaacacggtaaaacgatgactgaaaaagaaatcgtggactacgtcgcctcgcaggtgaccaccgctaaaaagttgcgtggtggcgtggtgtttgtggatgaagtgccgaaaggcctgacgggtaaacgtgatgcacgtaagattcgtgagatcttgatcaaggcgaagaaaggtggtaaatccaagttgtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z