LOCUS BBa_K890000 4956 bp DNA linear 22-Dec-2024 DEFINITION methanococcus shuttle vector pAW42 ACCESSION BBa_K890000 VERSION BBa_K890000.1 FEATURES Location/Qualifiers promoter 1..159 /label=PhmvA misc_feature 1930..1956 /label=Tmcr CDS 2414..3274 /label=amp misc_feature 3707..3929 /label=Eco Ori ORIGIN 1 ttcagctgat cgatcaaaat ataacataaa taacataggt ttaaataatt taaaggcata 61 ttttatataa acaattgtaa aatattggct tatgaaattt gttaaaattt agctaataag 121 ctattgatat tataatttta tagataacta ataggtgaaa tgcatgcacc accatcacca 181 tcacatcgaa ggtcgtgggc ccgccggcgg cgcgcctagg agatctcatg atatctagat 241 cctctagagg atgattaatt ttaagagagt cttaaaaaat cttcggaaaa tagtagtagg 301 taataaaaaa cgccctattc gtaatatact attcaaaact aaatattcat tgaaatattt 361 gggtaataat taatattaat ctaatattta gatattgaga tataataata acattttttt 421 aaaaaatcat ctattttcat cttaatttta tatattaaag cccatttttt gagtattcaa 481 attcaaatta ttgtgttatt aacatcttat atataaactt ttctatttaa tgttaatgaa 541 aaagtgaata tatatacata gagtaatgtt atgatgtata tatcaaaaaa ataggagtga 601 ttcctatggt aaagtatgaa gataagatat gtctatttga tgcgaaagga aaccaagtag 661 cagaagatgt accattggaa gctatcagcc cattaaataa cccaactata atggggatgg 721 ttaaaaatat taaaagaacc gttgctgttg acggatcccc agcttgcatg cctgcaggtc 781 gacatcgcgg ccgaaccggt tcgtgagccc ctgggacatc gccgccctcc agatcctcgt 841 ggaggaggcg ggcggcgtct gcaccgacct gctcggcggc tcgccccagc gcgggacggg 901 tgccctgtcc gccaacccgg agctgcaccg gctcgccgtg gaggccctcg ccgcccccgc 961 ggccgccacg accggtgccg ccaccatccc ctgacccacg cccctgaccc ctcacaagga 1021 gacgaccttc catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc 1081 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg 1141 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg 1201 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga 1261 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg 1321 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc 1381 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca 1441 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc 1501 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca 1561 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc 1621 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc 1681 atggctccga ccgaagccac ccggggcggc cccgccgacc ccgcacccgc ccccgaggcc 1741 caccgcgggg gacacaccga acacgccgac cctgctgaac acgcggcgca gttcggtgcc 1801 caggagcgga tcgggaatta attcgaagct gctggtgaaa gagaccctat cttacctgct 1861 aaaatctaag ttaattacta atttattatt aatttattat tagattgggt aaaatagtaa 1921 aagaaaacta aaggaaacct aatatggttt ccttttttta tatattttta attcactggg 1981 gggatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt ggtgcactct 2041 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc 2101 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt 2161 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa 2221 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac 2281 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 2341 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 2401 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 2461 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 2521 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 2581 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 2641 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 2701 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 2761 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 2821 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 2881 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 2941 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 3001 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 3061 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 3121 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 3181 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 3241 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 3301 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 3361 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 3421 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 3481 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 3541 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 3601 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 3661 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 3721 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 3781 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 3841 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 3901 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 3961 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 4021 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 4081 ttttgctcac atggtaccca ttatttagtt tttttttatc accacaacgt ataaaccccc 4141 tttcagaaga aaacagagaa tgaaaacaga ttttaattta tatatcgtat ctataattta 4201 gttttttttt attgcagttt tagaagtgcg tttgtcctgg agcgaaatac acgatatcgt 4261 gtagtctcct tcagatgaaa ataatatttt taattttcag aaatattact ggaaaaatac 4321 ctgaaaaata tttgaaaaaa aaacatacac ccagcgggga aaaatcgaac attttgaaaa 4381 agtctattta tagggtttaa acagggccaa gacctgcggg atatacccca aaaatgttcg 4441 ataaaaaaat cgaacatttt tcgaacattt tttataatgt tcgatgtgtg cgagtatgtt 4501 cgataaaaaa atcgaacatt ttgaaagaca atttgaagag atataaaaaa aataatctat 4561 tataatatag atatataata tgttcgatat gttcgatatt tttttatata ttatattata 4621 tttaaccttg tattgattat tgaataatat ttattattac tttttttgat ttcttgtaaa 4681 aaatcgaaca tattttacat attgtaaaaa ataaaaaaga tttaaaaaaa gtgtccgaaa 4741 aagttaaaca aacattatta ttaaaatgtt agtttaatat gtatcttgat atgctcttct 4801 ttctttcatg cttaaacttg tataactgta tgcttttttc atttccttta ttttgtcctt 4861 tcctttcttt gttatctggt aatatccttc cttctttcct gcatacttca aaaatccaag 4921 gttttttaag ttatcgagat aagaattact agttaa //