BBa_K891234
1
BBa_K891234
D168A Double Cysteine Mutant of Variola Virus Topoisomerase
2012-10-01T11:00:00Z
2015-05-08T01:13:41Z
This part comes from the smallpox genome. The cysteines have been mutated out and the 168th amino acid has been changed from aspartic acid to alanine. A his tag has been added to the end of this sequence.
This mutant version of topoisomerase recognizes the YCCTT motif in dsDNA. It cleaves after the last T in this motif, making a single stranded nick, and covalently binds to the 3' phosphate on that thymine.
false
false
_749_1155_1205_
0
10429
9
It's complicated
true
This part needs to be placed behind an inducible promoter, since it is toxic to the cell and its own plasmid.
false
Ryan Muller
annotation2211132
1
Stop Codon
range2211132
1
942
945
annotation2211543
1
D168A
range2211543
1
502
504
annotation2211130
1
Start codon
range2211130
1
1
3
BBa_K891234_sequence
1
atgcgtgcacttttttataaagatggtaaactgtttaccgataataattttttaaatcctgtatcagacaataatccagcgtatgaggttttgcaacatgttaaaattcctactcatttaacagatgtagtagtatatgaacaaacgtgggaggaggcgttaactagattaatttttgtgggaagtgattcaaaaggacgtagacaatacttttacggaaaaatgcatgtacagaatcgcaacgctaaaagagatcgtatttttgttagagtatataacgttatgaaacgaattaattgttttataaacaaaaatataaagaaatcatccacagattccaattatcagttggcagtttttatgttaatggaaactatgttttttattagatttggtaaaatgaaatatcttaaggagaatgaaacagtagggttattaacactaaaaaataaacacatagaaataagtcccgataaaatagttatcaagtttgtaggaaaggccaaagtttcacatgaatttgttgttcataagtctaatagactatataaaccgctattgaaactgacggatgattctagtcccgaagaatttctgttcaacaaactaagtgaacgaaaggtatacgaatgtatcaaacagtttggtattagaatcaaggatctccgaacgtatggagtcaattatacgtttttatataatttttggacaaatgtaaagtccatatctcctcttccatcaccaaaaaagttaatagcgttaactatcaaacaaactgctgaagtggtaggtcatactccatcaatttcaaaaagagcttatatggcaacgactattttagaaatggtaaaggataaaaattttttagatgtagtatctaaaactacgttcgatgaattcctatctatagtcgtagatcacgttaaatcatctacggatggatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z