BBa_K891234 1 BBa_K891234 D168A Double Cysteine Mutant of Variola Virus Topoisomerase 2012-10-01T11:00:00Z 2015-05-08T01:13:41Z This part comes from the smallpox genome. The cysteines have been mutated out and the 168th amino acid has been changed from aspartic acid to alanine. A his tag has been added to the end of this sequence. This mutant version of topoisomerase recognizes the YCCTT motif in dsDNA. It cleaves after the last T in this motif, making a single stranded nick, and covalently binds to the 3' phosphate on that thymine. false false _749_1155_1205_ 0 10429 9 It's complicated true This part needs to be placed behind an inducible promoter, since it is toxic to the cell and its own plasmid. false Ryan Muller annotation2211130 1 Start codon range2211130 1 1 3 annotation2211543 1 D168A range2211543 1 502 504 annotation2211132 1 Stop Codon range2211132 1 942 945 BBa_K891234_sequence 1 atgcgtgcacttttttataaagatggtaaactgtttaccgataataattttttaaatcctgtatcagacaataatccagcgtatgaggttttgcaacatgttaaaattcctactcatttaacagatgtagtagtatatgaacaaacgtgggaggaggcgttaactagattaatttttgtgggaagtgattcaaaaggacgtagacaatacttttacggaaaaatgcatgtacagaatcgcaacgctaaaagagatcgtatttttgttagagtatataacgttatgaaacgaattaattgttttataaacaaaaatataaagaaatcatccacagattccaattatcagttggcagtttttatgttaatggaaactatgttttttattagatttggtaaaatgaaatatcttaaggagaatgaaacagtagggttattaacactaaaaaataaacacatagaaataagtcccgataaaatagttatcaagtttgtaggaaaggccaaagtttcacatgaatttgttgttcataagtctaatagactatataaaccgctattgaaactgacggatgattctagtcccgaagaatttctgttcaacaaactaagtgaacgaaaggtatacgaatgtatcaaacagtttggtattagaatcaaggatctccgaacgtatggagtcaattatacgtttttatataatttttggacaaatgtaaagtccatatctcctcttccatcaccaaaaaagttaatagcgttaactatcaaacaaactgctgaagtggtaggtcatactccatcaatttcaaaaagagcttatatggcaacgactattttagaaatggtaaaggataaaaattttttagatgtagtatctaaaactacgttcgatgaattcctatctatagtcgtagatcacgttaaatcatctacggatggatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z