##gff-version 3
##sequence-region BBa_K892000 1 3734
BBa_K892000	.	engineered_region	117	1103	.	+	0	ID=BBa_Q04510;Name=BBa_Q04510
BBa_K892000	.	promoter	1055	1103	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_Q04510
BBa_K892000	.	promoter	1092	1097	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K892000	.	non_covalent_binding_site	1079	1095	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K892000	.	engineered_region	1055	1103	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K892000	.	non_covalent_binding_site	1055	1071	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K892000	.	promoter	1069	1074	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K892000	.	CDS	135	884	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_Q04510
BBa_K892000	.	tag	846	878	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K892000	.	engineered_tag	885	909	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K892000	.	CDS	138	845	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K892000	.	terminator	918	997	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04510
BBa_K892000	.	engineered_region	918	997	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K892000	.	stem_loop	929	972	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K892000	.	ribosome_entry_site	117	128	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04510
BBa_K892000	.	conserved_region	121	124	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K892000	.	terminator	1006	1046	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04510
BBa_K892000	.	engineered_region	1006	1046	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K892000	.	stem_loop	1013	1032	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K892000	.	polyA_site	1033	1046	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K892000	.	stop_codon	1039	1039	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K892000	.	promoter	1	108	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_K892000	.	promoter	75	80	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_K892000	.	non_covalent_binding_site	54	71	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K892000	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_K892000	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K892000	.	promoter	98	103	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K892000	.	engineered_region	1112	3734	.	+	0	ID=BBa_K325210;Name=BBa_K325210
BBa_K892000	.	sequence_alteration	2941	2941	.	+	0	ID=annotation2103099;Name=G to A: Ser286Asn;Parent=BBa_K325210
BBa_K892000	.	CDS	2085	3728	.	+	0	ID=annotation2080154;Name=Red luciferase (286) (L. cruciata);Parent=BBa_K325210
BBa_K892000	.	ribosome_entry_site	1112	1128	.	+	0	ID=annotation2080149;Name=rbs;Parent=BBa_K325210
BBa_K892000	.	CDS	1129	2055	.	+	0	ID=annotation2080150;Name=Luciferin regenerating enzyme (L. cruciata);Parent=BBa_K325210
BBa_K892000	.	stop_codon	2056	2061	.	+	0	ID=annotation2080151;Name=double stop codon;Parent=BBa_K325210
BBa_K892000	.	stop_codon	3729	3734	.	+	0	ID=annotation2080155;Name=double stop codon;Parent=BBa_K325210
BBa_K892000	.	ribosome_entry_site	2068	2084	.	+	0	ID=annotation2080153;Name=rbs;Parent=BBa_K325210
BBa_K892000	.	non_covalent_binding_site	2062	2067	.	+	0	ID=annotation2080152;Name=BamH1 site;Parent=BBa_K325210
>BBa_K892000
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttgga
ttattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatgagcacaaaaaagaaaccatta
acacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccagg
aatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgctta
taacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgag
atgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcaggga
tgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattc
tgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgtta
attctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttca
agaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagag
ttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgct
ttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctac
tagagttaaggaggtaaaaaaaatggccccgaccgtggaacaaattgtggagctgggtacgtatctgctggccgaaagc
ccgcactgggacgatgaaacgcaaagcctgtatttcgtcgatatcgttggtcgcagcgtgaacaaatacgttccgacga
ccaagacgcatacccaactgaaattcgacaagaatccgagcttcatcatcccggtgaagggttgttccgaccgttttat
cgtcagcctggaacgcgagatcaacctgctgacctgggacggcgcatcctctgcaccatccaagattgagaagatcgcg
gttttcgataatacgccagagaagagcgaaaaccgtctgaacgatggtaaggcagatccgctgggcaacctgtgggcgg
gtaccatgaatatgggttctgaccataccaccggcacgccggtgcgcggtaccctgagcagcctgagcaacaagcaagt
caaagagcacgtcagcgaggtctgcatcagcaatggcctggcatggagcaaggacctgaagaaattctattacattgat
agcgctgttcgtcaagttgaccagttcgatttcgatgcgaaaaacctgagcttgagcaatcgtcaaccgctgttcacct
ttgacaaacacggcatcatgggtagcccggatggtcagaccatcgatgcggagggtaacctgtgggttgccacctgtca
gggcgataaagtcctgaagattgatacttctaccccggaaaccctgctgggtattgtggagattccggaacatcaggtg
acgagcgtttgcatcggcggtgcagaactgaacgttttgtatgtgacgaccgcgtccattaaactgcctggtgcggatg
aaaccaagccgatgaaaggcgccatctataaagtcaccggtctgggtgtcaaaggtctgccgggtgaccgtgttaagct
gtaataaggatccttaaggaggtaaaaaaaatggagaacatggagaacgacgagaatattgtggttggtccgaaaccgt
tctacccgatcgaagagggcagcgcgggtacccagctgcgtaaatacatggaacgctacgcgaaactgggtgcgatcgc
gttcacgaatgcggttaccggcgtggactattcctatgcggagtatttggaaaagtcgtgttgtctgggcaaggcgtta
cagaattatggcctggtggtcgatggccgtatcgcgctgtgcagcgagaactgtgaagagtttttcattccggtgatcg
cgggtttgtttattggcgtgggcgttgcaccgaccaatgaaatctacacgctgcgcgaactggtgcactccctgggtat
ctctaagccgacgatcgttttcagctccaagaaaggtttggataaagttattacggtgcaaaagacggtcaccacgatc
aaaacgattgtgatcctggacagcaaagttgattaccgcggttatcaatgcctggatacgttcatcaagcgtaacaccc
cgccaggttttcaagcatctagcttcaaaacggtggaggttgaccgtaaagaacaagtcgcactgattatgaatagctc
gggtagcacgggcctgccaaagggtgtgcagctgacccatgaaaacaccgttacccgttttagccacgcgcgtgatccg
atttacggcaatcaagtgtcgccgggtacggctgttctgactgttgtcccttttcaccatggttttggcatgtttacca
ctctgggttacctgatttgcggttttcgcgtcgttatgctgaccaagtttgatgaagaaaccttcctgaaaaccctgca
agactacaagtgcactaatgtgattctggtcccgacgctgttcgcgatcctgaacaaaagcgagctgctgaataagtac
gacctgagcaatttggtggagattgcgagcggcggtgctccgttgagcaaagaagtaggcgaggcagtggcacgtcgct
ttaacctgcctggcgtccgtcaaggttacggtttgactgaaaccacgagcgcgattatcatcaccccggaaggtgacga
taaacctggcgctagcggtaaggtcgtgccgttgttcaaggctaaagttattgatctggacaccaagaagagcctgggc
ccgaatcgccgtggtgaagtttgcgttaagggcccgatgctgatgaaaggttatgtaaacaatccggaagcaacgaaag
agctgatcgacgaagagggctggctgcacactggcgatatcggctactacgatgaagagaaacactttttcattgttga
tcgcctgaaaagcctgatcaagtacaagggttaccaagtcccgccagcggagctggaaagcgtactgttgcagcacccg
tcgattttcgacgccggtgtggctggtgtgcctgatccggtggctggcgaactgccgggtgctgtcgtggtgctggaga
gcggtaagaatatgaccgaaaaagaagtcatggactatgtggcgagccaagtgtccaatgcaaaacgtctgcgtggcgg
tgttcgctttgtggacgaagtgccgaaaggtctgaccggcaaaattgacggtcgtgcaattcgtgagattctgaagaaa
ccggtcgcgaaaatgtaataa