BBa_K892001
type: ComponentDefinition
persistent identity: BBa K892001
display id: BBa_K892001
version: 1
was generated by: iGEM to SBOL conversion
description: red light responsive reporter driving red light luciferase
created: 2012-06-20T11:00:00Z
modified: 2015-05-08T01:13:41Z
mutable provenance: multiple biobricks
owned by: james
owned by: myers
mutable description: This part has the red light responsive reporter OmpR. That drives the inhibitory protein c1 from lambda phage. It also contains red light luciferase under the lambda phage promoter. Essentially lambda c1 switches the off signal to the on signal.
discontinued: false
dominant: false
group u list: _1156_
m user id: 0
owner id: 13850
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is cloned using standard assembly
star: false
creator: William Conrad
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_K325210
component: BBa_Q04510
component: BBa_R0082
sequence annotation: BBa_R0082
sequence annotation: BBa_Q04510
sequence annotation: BBa_K325210
sequence: https://synbiohub.org/public/igem/BBa_K892001_sequence/1

BBa_K325210
type: Component
persistent identity: component2176986
display id: component2176986
version: 1
top level: BBa_K892001
definition: BBa_K325210
access: public

BBa_Q04510
type: Component
persistent identity: component2176977
display id: component2176977
version: 1
top level: BBa_K892001
definition: BBa_Q04510
access: public

BBa_R0082
type: Component
persistent identity: component2176959
display id: component2176959
version: 1
top level: BBa_K892001
definition: OmpR
access: public

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2176959
display id: annotation2176959
version: 1
top level: BBa_K892001
location: https://synbiohub.org/public/igem/BBa_K892001/annotation2176959/range2176959/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K892001/annotation2176959/range2176959/1
type: Range
persistent identity: range2176959
display id: range2176959
version: 1
top level: BBa_K892001
direction: direction/reverse
start: 1
end: 108
orientation: inline

BBa_Q04510
type: SequenceAnnotation
persistent identity: annotation2176977
display id: annotation2176977
version: 1
top level: BBa_K892001
location: https://synbiohub.org/public/igem/BBa_K892001/annotation2176977/range2176977/1
component: BBa_Q04510

https://synbiohub.org/public/igem/BBa_K892001/annotation2176977/range2176977/1
type: Range
persistent identity: range2176977
display id: range2176977
version: 1
top level: BBa_K892001
direction: direction/reverse
start: 117
end: 1103
orientation: inline

BBa_K325210
type: SequenceAnnotation
persistent identity: annotation2176986
display id: annotation2176986
version: 1
top level: BBa_K892001
location: https://synbiohub.org/public/igem/BBa_K892001/annotation2176986/range2176986/1
component: BBa_K325210

https://synbiohub.org/public/igem/BBa_K892001/annotation2176986/range2176986/1
type: Range
persistent identity: range2176986
display id: range2176986
version: 1
top level: BBa_K892001
direction: direction/reverse
start: 1112
end: 3734
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
top level: OmpR
direction: direction/forward
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
top level: OmpR
direction: direction/forward
start: 54
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
top level: OmpR
direction: direction/forward
start: 75
end: 80
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
top level: OmpR
direction: direction/forward
start: 98
end: 103
orientation: inline

BBa_Q04510
type: ComponentDefinition
persistent identity: BBa Q04510
display id: BBa_Q04510
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.C0051.B0015.R0051)
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:14Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lambda cI QPI w/ strong RBS
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Inverter
role: SO:0000804
component: BBa_R0051
component: BBa_B0012
component: BBa_B0010
component: BBa_C0051
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0051
sequence: https://synbiohub.org/public/igem/BBa_Q04510_sequence/1

BBa_R0051
type: Component
persistent identity: component952926
display id: component952926
version: 1
top level: BBa_Q04510
definition: cI lam
access: public

BBa_B0012
type: Component
persistent identity: component952911
display id: component952911
version: 1
top level: BBa_Q04510
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component952901
display id: component952901
version: 1
top level: BBa_Q04510
definition: BBa_B0010
access: public

BBa_C0051
type: Component
persistent identity: component952895
display id: component952895
version: 1
top level: BBa_Q04510
definition: cI lam
access: public

BBa_B0034
type: Component
persistent identity: component952885
display id: component952885
version: 1
top level: BBa_Q04510
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation952885
display id: annotation952885
version: 1
top level: BBa_Q04510
location: https://synbiohub.org/public/igem/BBa_Q04510/annotation952885/range952885/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_Q04510/annotation952885/range952885/1
type: Range
persistent identity: range952885
display id: range952885
version: 1
top level: BBa_Q04510
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation952895
display id: annotation952895
version: 1
top level: BBa_Q04510
location: https://synbiohub.org/public/igem/BBa_Q04510/annotation952895/range952895/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_Q04510/annotation952895/range952895/1
type: Range
persistent identity: range952895
display id: range952895
version: 1
top level: BBa_Q04510
direction: direction/forward
start: 19
end: 768
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation952901
display id: annotation952901
version: 1
top level: BBa_Q04510
location: https://synbiohub.org/public/igem/BBa_Q04510/annotation952901/range952901/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q04510/annotation952901/range952901/1
type: Range
persistent identity: range952901
display id: range952901
version: 1
top level: BBa_Q04510
direction: direction/forward
start: 802
end: 881
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation952911
display id: annotation952911
version: 1
top level: BBa_Q04510
location: https://synbiohub.org/public/igem/BBa_Q04510/annotation952911/range952911/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q04510/annotation952911/range952911/1
type: Range
persistent identity: range952911
display id: range952911
version: 1
top level: BBa_Q04510
direction: direction/forward
start: 890
end: 930
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation952926
display id: annotation952926
version: 1
top level: BBa_Q04510
location: https://synbiohub.org/public/igem/BBa_Q04510/annotation952926/range952926/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_Q04510/annotation952926/range952926/1
type: Range
persistent identity: range952926
display id: range952926
version: 1
top level: BBa_Q04510
direction: direction/forward
start: 939
end: 987
orientation: inline

BBa_K325210
type: ComponentDefinition
persistent identity: BBa K325210
display id: BBa_K325210
version: 1
was generated by: iGEM to SBOL conversion
description: Red Firefly Luciferase and LRE L. Cruciata (E. coli optimised)
created: 2010-09-14T11:00:00Z
modified: 2015-05-08T01:12:02Z
mutable provenance: This was codon-optimised for E.coli and syntesised by DNA 2.0.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part is contains the coding sequence for a mutant light-emitting enzyme (luciferase) and luciferin regenerating enzyme (LRE) from the Japanese firefly, Luciola cruciata. It will make bacteria emit light at a reddish wavelength when placed under a promoter, provided the media is supplemented with luciferin. The luciferin regenerating enzyme will convert oxyluciferin into CHBT. CHBT is converted to more luciferin in the presence of D-cysteine.
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Codon optimisation, making compatible with BioBrick assembly format.
star: false
creator: Hannah Copley and Theo Sanderson
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: rbs
sequence annotation: Luciferin regenerating enzyme (L. cruciata)
sequence annotation: double stop codon
sequence annotation: BamH1 site
sequence annotation: Red luciferase (286) (L. cruciata)
sequence annotation: G to A: Ser286Asn
sequence: https://synbiohub.org/public/igem/BBa_K325210_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2080149
display id: annotation2080149
version: 1
top level: BBa_K325210
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080149/range2080149/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K325210/annotation2080149/range2080149/1
type: Range
persistent identity: range2080149
display id: range2080149
version: 1
top level: BBa_K325210
direction: direction/forward
start: 1
end: 17
orientation: inline

Luciferin regenerating enzyme (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2080150
display id: annotation2080150
version: 1
top level: BBa_K325210
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080150/range2080150/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K325210/annotation2080150/range2080150/1
type: Range
persistent identity: range2080150
display id: range2080150
version: 1
top level: BBa_K325210
direction: direction/forward
start: 18
end: 944
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2080151
display id: annotation2080151
version: 1
top level: BBa_K325210
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080151/range2080151/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K325210/annotation2080151/range2080151/1
type: Range
persistent identity: range2080151
display id: range2080151
version: 1
top level: BBa_K325210
direction: direction/forward
start: 945
end: 950
orientation: inline

BamH1 site
type: SequenceAnnotation
persistent identity: annotation2080152
display id: annotation2080152
version: 1
top level: BBa_K325210
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080152/range2080152/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K325210/annotation2080152/range2080152/1
type: Range
persistent identity: range2080152
display id: range2080152
version: 1
top level: BBa_K325210
direction: direction/forward
start: 951
end: 956
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2080153
display id: annotation2080153
version: 1
top level: BBa_K325210
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080153/range2080153/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K325210/annotation2080153/range2080153/1
type: Range
persistent identity: range2080153
display id: range2080153
version: 1
top level: BBa_K325210
direction: direction/forward
start: 957
end: 973
orientation: inline

Red luciferase (286) (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2080154
display id: annotation2080154
version: 1
top level: BBa_K325210
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080154/range2080154/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K325210/annotation2080154/range2080154/1
type: Range
persistent identity: range2080154
display id: range2080154
version: 1
top level: BBa_K325210
direction: direction/forward
start: 974
end: 2617
orientation: inline

G to A: Ser286Asn
type: SequenceAnnotation
persistent identity: annotation2103099
display id: annotation2103099
version: 1
top level: BBa_K325210
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2103099/range2103099/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K325210/annotation2103099/range2103099/1
type: Range
persistent identity: range2103099
display id: range2103099
version: 1
top level: BBa_K325210
direction: direction/forward
start: 1830
end: 1830
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2080155
display id: annotation2080155
version: 1
top level: BBa_K325210
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080155/range2080155/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K325210/annotation2080155/range2080155/1
type: Range
persistent identity: range2080155
display id: range2080155
version: 1
top level: BBa_K325210
direction: direction/forward
start: 2618
end: 2623
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

https://synbiohub.org/public/igem/BBa_K892001_sequence/1
type: Sequence
persistent identity: BBa K892001 sequence
display id: BBa_K892001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagttaaggaggtaaaaaaaatggccccgaccgtggaacaaattgtggagctgggtacgtatctgctggccgaaagcccgcactgggacgatgaaacgcaaagcctgtatttcgtcgatatcgttggtcgcagcgtgaacaaatacgttccgacgaccaagacgcatacccaactgaaattcgacaagaatccgagcttcatcatcccggtgaagggttgttccgaccgttttatcgtcagcctggaacgcgagatcaacctgctgacctgggacggcgcatcctctgcaccatccaagattgagaagatcgcggttttcgataatacgccagagaagagcgaaaaccgtctgaacgatggtaaggcagatccgctgggcaacctgtgggcgggtaccatgaatatgggttctgaccataccaccggcacgccggtgcgcggtaccctgagcagcctgagcaacaagcaagtcaaagagcacgtcagcgaggtctgcatcagcaatggcctggcatggagcaaggacctgaagaaattctattacattgatagcgctgttcgtcaagttgaccagttcgatttcgatgcgaaaaacctgagcttgagcaatcgtcaaccgctgttcacctttgacaaacacggcatcatgggtagcccggatggtcagaccatcgatgcggagggtaacctgtgggttgccacctgtcagggcgataaagtcctgaagattgatacttctaccccggaaaccctgctgggtattgtggagattccggaacatcaggtgacgagcgtttgcatcggcggtgcagaactgaacgttttgtatgtgacgaccgcgtccattaaactgcctggtgcggatgaaaccaagccgatgaaaggcgccat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0082_sequence/1
type: Sequence
persistent identity: BBa R0082 sequence
display id: BBa_R0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04510_sequence/1
type: Sequence
persistent identity: BBa Q04510 sequence
display id: BBa_Q04510_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K325210_sequence/1
type: Sequence
persistent identity: BBa K325210 sequence
display id: BBa_K325210_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaggaggtaaaaaaaatggccccgaccgtggaacaaattgtggagctgggtacgtatctgctggccgaaagcccgcactgggacgatgaaacgcaaagcctgtatttcgtcgatatcgttggtcgcagcgtgaacaaatacgttccgacgaccaagacgcatacccaactgaaattcgacaagaatccgagcttcatcatcccggtgaagggttgttccgaccgttttatcgtcagcctggaacgcgagatcaacctgctgacctgggacggcgcatcctctgcaccatccaagattgagaagatcgcggttttcgataatacgccagagaagagcgaaaaccgtctgaacgatggtaaggcagatccgctgggcaacctgtgggcgggtaccatgaatatgggttctgaccataccaccggcacgccggtgcgcggtaccctgagcagcctgagcaacaagcaagtcaaagagcacgtcagcgaggtctgcatcagcaatggcctggcatggagcaaggacctgaagaaattctattacattgatagcgctgttcgtcaagttgaccagttcgatttcgatgcgaaaaacctgagcttgagcaatcgtcaaccgctgttcacctttgacaaacacggcatcatgggtagcccggatggtcagaccatcgatgcggagggtaacctgtgggttgccacctgtcagggcgataaagtcctgaagattgatacttctaccccggaaaccctgctgggtattgtggagattccggaacatcaggtgacgagcgtttgcatcggcggtgcagaactgaacgttttgtatgtgacgaccgcgtccattaaactgcctggtgcggatgaaaccaagccgatgaaaggcgccatctataaagtcaccggtctgggtgtcaaaggtctgccgggtgaccgtgttaagctgtaataaggatccttaaggaggtaaaaaaaatggagaacatggagaacgacgagaatattgtggttggtccgaaaccgttctacccgatcgaagagggcagcgcgggtacccagctgcgtaaatacatggaacgctacgcgaaactgggtgcgatcgcgttcacgaatgcggttaccggcgtggactattcctatgcggagtatttggaaaagtcgtgttgtctgggcaaggcgttacagaattatggcctggtggtcgatggccgtatcgcgctgtgcagcgagaactgtgaagagtttttcattccggtgatcgcgggtttgtttattggcgtgggcgttgcaccgaccaatgaaatctacacgctgcgcgaactggtgcactccctgggtatctctaagccgacgatcgttttcagctccaagaaaggtttggataaagttattacggtgcaaaagacggtcaccacgatcaaaacgattgtgatcctggacagcaaagttgattaccgcggttatcaatgcctggatacgttcatcaagcgtaacaccccgccaggttttcaagcatctagcttcaaaacggtggaggttgaccgtaaagaacaagtcgcactgattatgaatagctcgggtagcacgggcctgccaaagggtgtgcagctgacccatgaaaacaccgttacccgttttagccacgcgcgtgatccgatttacggcaatcaagtgtcgccgggtacggctgttctgactgttgtcccttttcaccatggttttggcatgtttaccactctgggttacctgatttgcggttttcgcgtcgttatgctgaccaagtttgatgaagaaaccttcctgaaaaccctgcaagactacaagtgcactaatgtgattctggtcccgacgctgttcgcgatcctgaacaaaagcgagctgctgaataagtacgacctgagcaatttggtggagattgcgagcggcggtgctccgttgagcaaagaagtaggcgaggcagtggcacgtcgctttaacctgcctggcgtccgtcaaggttacg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z