BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K892002 1 BBa_K892002 Red Light responsive system, dual regulation 2012-06-20T11:00:00Z 2015-05-08T01:13:41Z Registry of biological parts Pbad regulated phycobilins (ho1 and pcyA) followed by Ptet regulated light sensor (Cph8). This should allow repressed Pbad to give low levels of accessory proteins and derepressed Ptet to crank out lots of light sensor protein. false false _1156_ 0 13901 9 Not in stock false can be added to bacteria containing C resistance but not vice versa false Xindi Guo component2177085 1 BBa_B0012 component2177093 1 BBa_K098011 component2177084 1 BBa_K098010 component2177094 1 BBa_B0012 annotation2177094 1 BBa_B0012 range2177094 1 2514 2554 annotation2177084 1 BBa_K098010 range2177084 1 1 1531 annotation2177093 1 BBa_K098011 range2177093 1 1589 2505 annotation2177085 1 BBa_B0012 range2177085 1 1540 1580 BBa_K098011 1 BBa_K098011 E. coli ompR, expressed at low level 2008-10-25T11:00:00Z 2015-05-08T01:08:41Z E. coli genome (DH5alpha) *ompR, E. coli two-component signal transduction system used along with EnvZ *controls expression of genes under ompC and ompF promoters *doi:10.1016/j.physletb.2003.10.071 false false _196_ 0 3389 9 It's complicated true obtained by PCR true Harvard iGEM 08 annotation1990311 1 BBa_B0015 (terminator) range1990311 1 789 888 annotation1990307 1 BBa_J23113 range1990307 1 1 35 annotation1990309 1 BBa_B0032 range1990309 1 44 56 annotation1990310 1 ompR range1990310 1 63 782 BBa_K098010 1 BBa_K098010 HO-pcyA, low expression 2008-10-25T11:00:00Z 2015-05-08T01:08:41Z pPCB-AmpR plasmid from Jeff Tabor (?) *Heme oxygenase and phycocyanobilin:ferredoxin oxidoreductase *Used for light sensing system false false _196_ 0 3389 9 It's complicated true obtained via PCR true Harvard iGEM 08 annotation1990294 1 RBS pprotet range1990294 1 630 643 annotation1990296 1 BBa_B0015 (terminator) range1990296 1 1403 1502 annotation1990292 1 BBa_B0032 range1990292 1 44 56 annotation1990291 1 BBa_J23113 range1990291 1 1 35 annotation1990293 1 HO range1990293 1 63 629 annotation1990295 1 pcyA range1990295 1 650 1396 BBa_K098010_sequence 1 ctgatggctagctcagtcctagggattatgctagctactagagtcacacaggaaaggtgcacatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagttaaagaggagaaaggatccatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataaggtaccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K098011_sequence 1 ctgatggctagctcagtcctagggattatgctagctactagagtcacacaggaaaggtgcacatgcaagagaactacaagattctggtggtcgatgacgacatgcgcctgcgtgcgctgctggaacgttatctcaccgaacaaggcttccaggttcgaagcgtcgctaatgcagaacagatggatcgcctgctgactcgtgaatctttccatcttatggtactggatttaatgttacctggtgaagatggcttgtcgatttgccgacgtcttcgtagtcagagcaacccgatgccgatcattatggtgacggcgaaaggggaagaagtggaccgtatcgtaggcctggagattggcgctgacgactacattccaaaaccgtttaacccgcgtgaactgctggcccgtatccgtgcggtgctgcgtcgtcaggcgaacgaactgccaggcgcaccgtcacaggaagaggcggtaattgctttcggtaagttcaaacttaacctcggtacgcgcgaaatgttccgcgaagacgagccgatgccgctcaccagcggtgagtttgcggtactgaaggcactggtcagccatccgcgtgagccgctctcccgcgataagctgatgaaccttgcccgtggtcgtgaatattccgcaatggaacgctccatcgacgtgcagatttcgcgtctgcgccgcatggtggaagaagatccagcgcatccgcgttacattcagaccgtctggggtctgggctacgtctttgtaccggacggctctaaagcatgaggtaccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K892002_sequence 1 ctgatggctagctcagtcctagggattatgctagctactagagtcacacaggaaaggtgcacatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagttaaagaggagaaaggatccatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataaggtaccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctgatggctagctcagtcctagggattatgctagctactagagtcacacaggaaaggtgcacatgcaagagaactacaagattctggtggtcgatgacgacatgcgcctgcgtgcgctgctggaacgttatctcaccgaacaaggcttccaggttcgaagcgtcgctaatgcagaacagatggatcgcctgctgactcgtgaatctttccatcttatggtactggatttaatgttacctggtgaagatggcttgtcgatttgccgacgtcttcgtagtcagagcaacccgatgccgatcattatggtgacggcgaaaggggaagaagtggaccgtatcgtaggcctggagattggcgctgacgactacattccaaaaccgtttaacccgcgtgaactgctggcccgtatccgtgcggtgctgcgtcgtcaggcgaacgaactgccaggcgcaccgtcacaggaagaggcggtaattgctttcggtaagttcaaacttaacctcggtacgcgcgaaatgttccgcgaagacgagccgatgccgctcaccagcggtgagtttgcggtactgaaggcactggtcagccatccgcgtgagccgctctcccgcgataagctgatgaaccttgcccgtggtcgtgaatattccgcaatggaacgctccatcgacgtgcagatttcgcgtctgcgccgcatggtggaagaagatccagcgcatccgcgttacattcagaccgtctggggtctgggctacgtctttgtaccggacggctctaaagcatgaggtaccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z