##gff-version 3
##sequence-region BBa_K892003 1 4118
BBa_K892003	.	engineered_region	1496	4118	.	+	0	ID=BBa_K325210;Name=BBa_K325210
BBa_K892003	.	ribosome_entry_site	1496	1512	.	+	0	ID=annotation2080149;Name=rbs;Parent=BBa_K325210
BBa_K892003	.	CDS	1513	2439	.	+	0	ID=annotation2080150;Name=Luciferin regenerating enzyme (L. cruciata);Parent=BBa_K325210
BBa_K892003	.	CDS	2469	4112	.	+	0	ID=annotation2080154;Name=Red luciferase (286) (L. cruciata);Parent=BBa_K325210
BBa_K892003	.	ribosome_entry_site	2452	2468	.	+	0	ID=annotation2080153;Name=rbs;Parent=BBa_K325210
BBa_K892003	.	non_covalent_binding_site	2446	2451	.	+	0	ID=annotation2080152;Name=BamH1 site;Parent=BBa_K325210
BBa_K892003	.	sequence_alteration	3325	3325	.	+	0	ID=annotation2103099;Name=G to A: Ser286Asn;Parent=BBa_K325210
BBa_K892003	.	stop_codon	2440	2445	.	+	0	ID=annotation2080151;Name=double stop codon;Parent=BBa_K325210
BBa_K892003	.	stop_codon	4113	4118	.	+	0	ID=annotation2080155;Name=double stop codon;Parent=BBa_K325210
BBa_K892003	.	promoter	1	108	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_K892003	.	promoter	98	103	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K892003	.	promoter	75	80	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_K892003	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_K892003	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K892003	.	non_covalent_binding_site	54	71	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K892003	.	engineered_region	117	1487	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121
BBa_K892003	.	terminator	1296	1424	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_K892003	.	terminator	1296	1375	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K892003	.	stem_loop	1307	1350	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K892003	.	engineered_region	1296	1375	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K892003	.	terminator	1384	1424	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K892003	.	engineered_region	1384	1424	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K892003	.	stop_codon	1417	1417	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K892003	.	stem_loop	1391	1410	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K892003	.	polyA_site	1411	1424	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K892003	.	promoter	1433	1486	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_K892003	.	promoter	1452	1457	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K892003	.	promoter	1475	1480	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K892003	.	operator	1435	1451	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K892003	.	operator	1458	1474	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K892003	.	engineered_region	1433	1486	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K892003	.	CDS	135	1262	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_K892003	.	engineered_tag	1263	1287	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K892003	.	CDS	135	1262	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K892003	.	tag	1224	1262	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K892003	.	engineered_region	135	1262	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K892003	.	ribosome_entry_site	117	128	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_K892003	.	conserved_region	121	124	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K892003
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttgga
ttattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacg
ttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctg
cgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcggg
caaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaa
tctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcgg
tgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtgga
agctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccat
gaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaa
gttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacg
ggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatg
ctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatct
cggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcct
gctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtc
tcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgc
agctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataact
ctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagttaagg
aggtaaaaaaaatggccccgaccgtggaacaaattgtggagctgggtacgtatctgctggccgaaagcccgcactggga
cgatgaaacgcaaagcctgtatttcgtcgatatcgttggtcgcagcgtgaacaaatacgttccgacgaccaagacgcat
acccaactgaaattcgacaagaatccgagcttcatcatcccggtgaagggttgttccgaccgttttatcgtcagcctgg
aacgcgagatcaacctgctgacctgggacggcgcatcctctgcaccatccaagattgagaagatcgcggttttcgataa
tacgccagagaagagcgaaaaccgtctgaacgatggtaaggcagatccgctgggcaacctgtgggcgggtaccatgaat
atgggttctgaccataccaccggcacgccggtgcgcggtaccctgagcagcctgagcaacaagcaagtcaaagagcacg
tcagcgaggtctgcatcagcaatggcctggcatggagcaaggacctgaagaaattctattacattgatagcgctgttcg
tcaagttgaccagttcgatttcgatgcgaaaaacctgagcttgagcaatcgtcaaccgctgttcacctttgacaaacac
ggcatcatgggtagcccggatggtcagaccatcgatgcggagggtaacctgtgggttgccacctgtcagggcgataaag
tcctgaagattgatacttctaccccggaaaccctgctgggtattgtggagattccggaacatcaggtgacgagcgtttg
catcggcggtgcagaactgaacgttttgtatgtgacgaccgcgtccattaaactgcctggtgcggatgaaaccaagccg
atgaaaggcgccatctataaagtcaccggtctgggtgtcaaaggtctgccgggtgaccgtgttaagctgtaataaggat
ccttaaggaggtaaaaaaaatggagaacatggagaacgacgagaatattgtggttggtccgaaaccgttctacccgatc
gaagagggcagcgcgggtacccagctgcgtaaatacatggaacgctacgcgaaactgggtgcgatcgcgttcacgaatg
cggttaccggcgtggactattcctatgcggagtatttggaaaagtcgtgttgtctgggcaaggcgttacagaattatgg
cctggtggtcgatggccgtatcgcgctgtgcagcgagaactgtgaagagtttttcattccggtgatcgcgggtttgttt
attggcgtgggcgttgcaccgaccaatgaaatctacacgctgcgcgaactggtgcactccctgggtatctctaagccga
cgatcgttttcagctccaagaaaggtttggataaagttattacggtgcaaaagacggtcaccacgatcaaaacgattgt
gatcctggacagcaaagttgattaccgcggttatcaatgcctggatacgttcatcaagcgtaacaccccgccaggtttt
caagcatctagcttcaaaacggtggaggttgaccgtaaagaacaagtcgcactgattatgaatagctcgggtagcacgg
gcctgccaaagggtgtgcagctgacccatgaaaacaccgttacccgttttagccacgcgcgtgatccgatttacggcaa
tcaagtgtcgccgggtacggctgttctgactgttgtcccttttcaccatggttttggcatgtttaccactctgggttac
ctgatttgcggttttcgcgtcgttatgctgaccaagtttgatgaagaaaccttcctgaaaaccctgcaagactacaagt
gcactaatgtgattctggtcccgacgctgttcgcgatcctgaacaaaagcgagctgctgaataagtacgacctgagcaa
tttggtggagattgcgagcggcggtgctccgttgagcaaagaagtaggcgaggcagtggcacgtcgctttaacctgcct
ggcgtccgtcaaggttacggtttgactgaaaccacgagcgcgattatcatcaccccggaaggtgacgataaacctggcg
ctagcggtaaggtcgtgccgttgttcaaggctaaagttattgatctggacaccaagaagagcctgggcccgaatcgccg
tggtgaagtttgcgttaagggcccgatgctgatgaaaggttatgtaaacaatccggaagcaacgaaagagctgatcgac
gaagagggctggctgcacactggcgatatcggctactacgatgaagagaaacactttttcattgttgatcgcctgaaaa
gcctgatcaagtacaagggttaccaagtcccgccagcggagctggaaagcgtactgttgcagcacccgtcgattttcga
cgccggtgtggctggtgtgcctgatccggtggctggcgaactgccgggtgctgtcgtggtgctggagagcggtaagaat
atgaccgaaaaagaagtcatggactatgtggcgagccaagtgtccaatgcaaaacgtctgcgtggcggtgttcgctttg
tggacgaagtgccgaaaggtctgaccggcaaaattgacggtcgtgcaattcgtgagattctgaagaaaccggtcgcgaa
aatgtaataa