LOCUS BBa_K892800 2781 bp DNA linear 25-Nov-2024 DEFINITION Blue Light Sensor, TetR Inverter and sfGFP Output ACCESSION BBa_K892800 VERSION BBa_K892800.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..223 /label=RBS-1\Strong RBS 216..220 /label=RBS misc_feature 209..223 /label=BBa_B0030 RBS 209..223 /label=BBa_B0030 CDS 230..916 /label=BBa_K892801 stem_loop 936..979 /label=stem_loop misc_feature 925..1004 /label=BBa_B0010 terminator 925..1004 /label=BBa_B0010 stem_loop 1020..1039 /label=T7 TE misc_feature 1046..1046 /label=stop polyA_site 1040..1053 /label=polya misc_feature 1013..1053 /label=BBa_B0012 terminator 1013..1053 /label=BBa_B0012 promoter 1062..1146 /label=BBa_K191007 RBS 1155..1165 /label=RBS-4\Weaker RBS 1161..1164 /label=RBS misc_feature 1155..1165 /label=BBa_B0033 RBS 1155..1165 /label=BBa_B0033 CDS 1175..1791 /label=tetR misc_feature 1792..1825 /label=SsrA misc_feature 1832..1856 /label=Help:Barcodes CDS 1172..1856 /label=BBa_C0040 stem_loop 1876..1919 /label=stem_loop misc_feature 1865..1944 /label=BBa_B0010 terminator 1865..1944 /label=BBa_B0010 stem_loop 1960..1979 /label=T7 TE misc_feature 1986..1986 /label=stop polyA_site 1980..1993 /label=polya misc_feature 1953..1993 /label=BBa_B0012 terminator 1953..1993 /label=BBa_B0012 misc_feature 2002..2020 /label=TetR 1 misc_feature 2002..2055 /label=BBa_R0040 promoter 2021..2026 /label=-35 misc_feature 2027..2045 /label=TetR 2 promoter 2044..2049 /label=-10 promoter 2002..2055 /label=BBa_R0040 misc_feature 2062..2064 /label=start CDS 2062..2781 /label=superfolder GFP coding region misc_feature 2776..2781 /label=stop CDS 2062..2781 /label=BBa_I746916 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgttggctac 241 tacacttgaa cgtattgaga agaactttgt cattactgac ccaaggttgc cagataatcc 301 cattatattc gcgtccgata gtttcttgca gttgacagaa tatagccgtg aagaaatttt 361 gggaagaaac tgcaggtttc tacaaggtcc tgaaactgat cgcgcgacag tgagaaaaat 421 tagagatgcc atagataacc aaacagaggt cactgttcag ctgattaatt atacaaagag 481 tggtaaaaag ttctggaacc tctttcactt gcagcctatg cgagatcaga agggagatgt 541 ccagtacttt attggggttc agttggatgg aactgagcat gtccgagatg ctgccgagag 601 agagggagtc atgctgatta agaaaactgc agaaaatatt gatgaggcgg catttgtcga 661 cctgcttaag aatgcctacc aaaacgatct ccatttaccg ttgttaaatc tgatgctgac 721 gccagatgag cgcgaagcgt tggggactcg cgtgcgtatt gtcgaagagc tgttgcgcgg 781 cgaaatgagc cagcgtgagt taaaaaatga actcggcgcg ggcatcgcga cgattacgcg 841 tggatctaac agcctgaaag ccgcgcccgt ggagctgcgc cagtggctgg aagaggtgtt 901 gctgaaaagc gattgatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 961 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 1021 gctcaccttc gggtgggcct ttctgcgttt atatactaga gtggcaaata ttctgaaatg 1081 agctgttgac aattaatcat cgaactagtt aactagtacg caagttcacg taaaaagggt 1141 atcgactact agagtcacac aggactacta gatgtccaga ttagataaaa gtaaagtgat 1201 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact 1261 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc 1321 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt 1381 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt 1441 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta 1501 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc 1561 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga 1621 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt 1681 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct 1741 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa 1801 cgacgaaaac tacgctttag tagcttaata acactgatag tgctagtgta gatcactact 1861 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1921 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 1981 ttctgcgttt atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat 2041 agagatactg agcactacta gatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct 2101 attctggtgg aactggatgg tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt 2161 gaaggtgacg caactaatgg taaactgacg ctgaagttca tctgtactac tggtaaactg 2221 ccggtacctt ggccgactct ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt 2281 tatccggacc atatgaagca gcatgacttc ttcaagtccg ccatgccgga aggctatgtg 2341 caggaacgca cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa 2401 tttgaaggcg ataccctggt aaaccgcatt gagctgaaag gcattgactt taaagaagac 2461 ggcaatatcc tgggccataa gctggaatac aattttaaca gccacaatgt ttacatcacc 2521 gccgataaac aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa cgtggaggat 2581 ggcagcgtgc agctggctga tcactaccag caaaacactc caatcggtga tggtcctgtt 2641 ctgctgccag acaatcacta tctgagcacg caaagcgttc tgtctaaaga tccgaacgag 2701 aaacgcgatc atatggttct gctggagttc gtaaccgcag cgggcatcac gcatggtatg 2761 gatgaactgt acaaatgatg a //