##gff-version 3
##sequence-region BBa_K892800 1 2781
BBa_K892800	.	CDS	1172	1856	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K892800	.	CDS	1175	1791	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K892800	.	tag	1792	1825	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K892800	.	engineered_tag	1832	1856	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K892800	.	ribosome_entry_site	1155	1165	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K892800	.	engineered_region	1155	1165	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K892800	.	ribosome_entry_site	1155	1165	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K892800	.	ribosome_entry_site	1161	1164	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K892800	.	terminator	1865	1944	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K892800	.	stem_loop	1876	1919	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K892800	.	engineered_region	1865	1944	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K892800	.	promoter	2002	2055	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K892800	.	promoter	2021	2026	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K892800	.	promoter	2044	2049	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K892800	.	non_covalent_binding_site	2002	2020	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K892800	.	engineered_region	2002	2055	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K892800	.	non_covalent_binding_site	2027	2045	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K892800	.	terminator	1953	1993	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K892800	.	polyA_site	1980	1993	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K892800	.	stem_loop	1960	1979	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K892800	.	engineered_region	1953	1993	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K892800	.	stop_codon	1986	1986	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K892800	.	terminator	925	1004	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K892800	.	stem_loop	936	979	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K892800	.	engineered_region	925	1004	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K892800	.	CDS	230	916	.	+	0	ID=BBa_K892801;Name=BBa_K892801
BBa_K892800	.	CDS	2062	2781	.	+	0	ID=BBa_I746916;Name=BBa_I746916
BBa_K892800	.	stop_codon	2776	2781	.	+	0	ID=annotation1977535;Name=stop;Parent=BBa_I746916
BBa_K892800	.	CDS	2062	2781	.	+	0	ID=annotation1977534;Name=superfolder GFP coding region;Parent=BBa_I746916
BBa_K892800	.	start_codon	2062	2064	.	+	0	ID=annotation1977533;Name=start;Parent=BBa_I746916
BBa_K892800	.	promoter	1062	1146	.	+	0	ID=BBa_K191007;Name=BBa_K191007
BBa_K892800	.	terminator	1013	1053	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K892800	.	polyA_site	1040	1053	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K892800	.	stem_loop	1020	1039	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K892800	.	engineered_region	1013	1053	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K892800	.	stop_codon	1046	1046	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K892800	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K892800	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K892800	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K892800	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K892800	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K892800	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K892800	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K892800	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K892800	.	ribosome_entry_site	209	223	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K892800	.	ribosome_entry_site	209	223	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K892800	.	ribosome_entry_site	216	220	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K892800	.	engineered_region	209	223	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
>BBa_K892800
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgttggc
tactacacttgaacgtattgagaagaactttgtcattactgacccaaggttgccagataatcccattatattcgcgtcc
gatagtttcttgcagttgacagaatatagccgtgaagaaattttgggaagaaactgcaggtttctacaaggtcctgaaa
ctgatcgcgcgacagtgagaaaaattagagatgccatagataaccaaacagaggtcactgttcagctgattaattatac
aaagagtggtaaaaagttctggaacctctttcacttgcagcctatgcgagatcagaagggagatgtccagtactttatt
ggggttcagttggatggaactgagcatgtccgagatgctgccgagagagagggagtcatgctgattaagaaaactgcag
aaaatattgatgaggcggcatttgtcgacctgcttaagaatgcctaccaaaacgatctccatttaccgttgttaaatct
gatgctgacgccagatgagcgcgaagcgttggggactcgcgtgcgtattgtcgaagagctgttgcgcggcgaaatgagc
cagcgtgagttaaaaaatgaactcggcgcgggcatcgcgacgattacgcgtggatctaacagcctgaaagccgcgcccg
tggagctgcgccagtggctggaagaggtgttgctgaaaagcgattgatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagtggcaaatattctgaaatgagctgttgacaattaatcatcgaact
agttaactagtacgcaagttcacgtaaaaagggtatcgactactagagtcacacaggactactagatgtccagattaga
taaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcc
cagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattg
agatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaa
aagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtat
gaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctg
tggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactac
tgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggc
cttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctt
tagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagca
ctactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggt
cataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactg
gtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggacca
tatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgac
ggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgact
ttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataa
acaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactac
cagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtcta
aagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatga
actgtacaaatgatga