LOCUS BBa_K892999 3734 bp DNA linear 25-Nov-2024 DEFINITION red light responsive inverter driving luciferase ACCESSION BBa_K892999 VERSION BBa_K892999.1 FEATURES Location/Qualifiers misc_feature 13..30 /label=C1 OmpR misc_feature 34..51 /label=C2 OmpR misc_feature 54..71 /label=C3 OmpR promoter 75..80 /label=-35 promoter 98..103 /label=-10 promoter 1..108 /label=BBa_R0082 misc_feature 121..124 /label=conserved RBS 117..128 /label=BBa_B0034 CDS 138..845 /label=cI lambda misc_feature 846..878 /label=LVA misc_feature 885..909 /label=Help:Barcodes CDS 135..884 /label=BBa_C0051 stem_loop 929..972 /label=stem_loop misc_feature 918..997 /label=BBa_B0010 terminator 918..997 /label=BBa_B0010 stem_loop 1013..1032 /label=T7 TE misc_feature 1039..1039 /label=stop polyA_site 1033..1046 /label=polya misc_feature 1006..1046 /label=BBa_B0012 terminator 1006..1046 /label=BBa_B0012 promoter 1092..1097 /label=-10 promoter 1069..1074 /label=-35 misc_feature 1079..1095 /label=OR1 misc_feature 1055..1071 /label=OR2 misc_feature 1055..1103 /label=BBa_R0051 promoter 1055..1103 /label=BBa_R0051 misc_feature 117..1103 /label=BBa_Q04510 RBS 1112..1128 /label=rbs CDS 1129..2055 /label=Luciferin regenerating enzyme (L. cruciata) misc_feature 2056..2061 /label=double stop codon misc_feature 2062..2067 /label=BamH1 site RBS 2068..2084 /label=rbs CDS 2085..3728 /label=Red luciferase (286) (L. cruciata) misc_feature 3729..3734 /label=double stop codon misc_feature 2941..2941 /label=G to A: Ser286Asn misc_feature 1112..3734 /label=BBa_K325210 ORIGIN 1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta 61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag 121 aggagaaata ctagatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg 181 cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat 241 ctgtcgcaga caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca 301 atgcattaaa tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag 361 aatttagccc ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc 421 cgtcacttag aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct 481 cacctgagct tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa 541 aagccagtga ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag 601 gctccaagcc aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg 661 agccaggtga tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga 721 tcagggatag cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat 781 gcaatgagag ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt 841 ttggcgctgc aaacgacgaa aactacgctt tagtagctta ataacgctga tagtgctagt 901 gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 961 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1021 ttcgggtggg cctttctgcg tttatatact agagtaacac cgtgcgtgtt gactatttta 1081 cctctggcgg tgataatggt tgctactaga gttaaggagg taaaaaaaat ggccccgacc 1141 gtggaacaaa ttgtggagct gggtacgtat ctgctggccg aaagcccgca ctgggacgat 1201 gaaacgcaaa gcctgtattt cgtcgatatc gttggtcgca gcgtgaacaa atacgttccg 1261 acgaccaaga cgcataccca actgaaattc gacaagaatc cgagcttcat catcccggtg 1321 aagggttgtt ccgaccgttt tatcgtcagc ctggaacgcg agatcaacct gctgacctgg 1381 gacggcgcat cctctgcacc atccaagatt gagaagatcg cggttttcga taatacgcca 1441 gagaagagcg aaaaccgtct gaacgatggt aaggcagatc cgctgggcaa cctgtgggcg 1501 ggtaccatga atatgggttc tgaccatacc accggcacgc cggtgcgcgg taccctgagc 1561 agcctgagca acaagcaagt caaagagcac gtcagcgagg tctgcatcag caatggcctg 1621 gcatggagca aggacctgaa gaaattctat tacattgata gcgctgttcg tcaagttgac 1681 cagttcgatt tcgatgcgaa aaacctgagc ttgagcaatc gtcaaccgct gttcaccttt 1741 gacaaacacg gcatcatggg tagcccggat ggtcagacca tcgatgcgga gggtaacctg 1801 tgggttgcca cctgtcaggg cgataaagtc ctgaagattg atacttctac cccggaaacc 1861 ctgctgggta ttgtggagat tccggaacat caggtgacga gcgtttgcat cggcggtgca 1921 gaactgaacg ttttgtatgt gacgaccgcg tccattaaac tgcctggtgc ggatgaaacc 1981 aagccgatga aaggcgccat ctataaagtc accggtctgg gtgtcaaagg tctgccgggt 2041 gaccgtgtta agctgtaata aggatcctta aggaggtaaa aaaaatggag aacatggaga 2101 acgacgagaa tattgtggtt ggtccgaaac cgttctaccc gatcgaagag ggcagcgcgg 2161 gtacccagct gcgtaaatac atggaacgct acgcgaaact gggtgcgatc gcgttcacga 2221 atgcggttac cggcgtggac tattcctatg cggagtattt ggaaaagtcg tgttgtctgg 2281 gcaaggcgtt acagaattat ggcctggtgg tcgatggccg tatcgcgctg tgcagcgaga 2341 actgtgaaga gtttttcatt ccggtgatcg cgggtttgtt tattggcgtg ggcgttgcac 2401 cgaccaatga aatctacacg ctgcgcgaac tggtgcactc cctgggtatc tctaagccga 2461 cgatcgtttt cagctccaag aaaggtttgg ataaagttat tacggtgcaa aagacggtca 2521 ccacgatcaa aacgattgtg atcctggaca gcaaagttga ttaccgcggt tatcaatgcc 2581 tggatacgtt catcaagcgt aacaccccgc caggttttca agcatctagc ttcaaaacgg 2641 tggaggttga ccgtaaagaa caagtcgcac tgattatgaa tagctcgggt agcacgggcc 2701 tgccaaaggg tgtgcagctg acccatgaaa acaccgttac ccgttttagc cacgcgcgtg 2761 atccgattta cggcaatcaa gtgtcgccgg gtacggctgt tctgactgtt gtcccttttc 2821 accatggttt tggcatgttt accactctgg gttacctgat ttgcggtttt cgcgtcgtta 2881 tgctgaccaa gtttgatgaa gaaaccttcc tgaaaaccct gcaagactac aagtgcacta 2941 atgtgattct ggtcccgacg ctgttcgcga tcctgaacaa aagcgagctg ctgaataagt 3001 acgacctgag caatttggtg gagattgcga gcggcggtgc tccgttgagc aaagaagtag 3061 gcgaggcagt ggcacgtcgc tttaacctgc ctggcgtccg tcaaggttac ggtttgactg 3121 aaaccacgag cgcgattatc atcaccccgg aaggtgacga taaacctggc gctagcggta 3181 aggtcgtgcc gttgttcaag gctaaagtta ttgatctgga caccaagaag agcctgggcc 3241 cgaatcgccg tggtgaagtt tgcgttaagg gcccgatgct gatgaaaggt tatgtaaaca 3301 atccggaagc aacgaaagag ctgatcgacg aagagggctg gctgcacact ggcgatatcg 3361 gctactacga tgaagagaaa cactttttca ttgttgatcg cctgaaaagc ctgatcaagt 3421 acaagggtta ccaagtcccg ccagcggagc tggaaagcgt actgttgcag cacccgtcga 3481 ttttcgacgc cggtgtggct ggtgtgcctg atccggtggc tggcgaactg ccgggtgctg 3541 tcgtggtgct ggagagcggt aagaatatga ccgaaaaaga agtcatggac tatgtggcga 3601 gccaagtgtc caatgcaaaa cgtctgcgtg gcggtgttcg ctttgtggac gaagtgccga 3661 aaggtctgac cggcaaaatt gacggtcgtg caattcgtga gattctgaag aaaccggtcg 3721 cgaaaatgta ataa //