BBa_Q04510 1 BBa_Q04510 QPI (B0034.C0051.B0015.R0051) 2003-12-02T12:00:00Z 2015-05-08T01:14:14Z Released HQ 2013 Lambda cI QPI w/ strong RBS false true _1_ 0 24 7 In stock false true Caitlin Conboy and Jennifer Braff component952911 1 BBa_B0012 component952926 1 BBa_R0051 component952895 1 BBa_C0051 component952885 1 BBa_B0034 component952901 1 BBa_B0010 annotation952885 1 BBa_B0034 range952885 1 1 12 annotation952926 1 BBa_R0051 range952926 1 939 987 annotation952895 1 BBa_C0051 range952895 1 19 768 annotation952901 1 BBa_B0010 range952901 1 802 881 annotation952911 1 BBa_B0012 range952911 1 890 930 BBa_C0051 1 cI lam cI repressor from E. coli phage lambda (+LVA) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Released HQ 2013 Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).</P> false false _1_ 0 24 7 In stock false References (unparsed) here: <p><a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000</P> <P><a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001</P> <p></p> <P> References (unparsed) here: <p><a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000</P> <P><a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001</P> <p></p> <P>BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.<P> true Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross annotation23334 1 cI lambda range23334 1 4 711 annotation2213991 1 Help:Barcodes range2213991 1 751 775 annotation23335 1 LVA range23335 1 712 744 BBa_R0082 1 OmpR Promoter (OmpR, positive) 2004-01-27T12:00:00Z 2015-05-08T01:14:15Z NC_000193 E. coli K12 Released HQ 2013 Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. false false _1_ 0 24 7 In stock false The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown. true Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004) annotation301156 1 C3 OmpR range301156 1 54 71 annotation301155 1 C2 OmpR range301155 1 34 51 annotation301166 1 -35 range301166 1 75 80 annotation301154 1 C1 OmpR range301154 1 13 30 annotation301167 1 -10 range301167 1 98 103 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_R0051 1 cI lam promoter (lambda cI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z <a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Released HQ 2013 The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).</P> false true _1_ 0 24 7 In stock false <P> <P>In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (<b><font color="red">More details needed here! DE, 2/24/03</font></b>)<P> Incompatible with host expressing cI repressor. true Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross annotation7067 1 BBa_R0051 range7067 1 1 49 annotation2023 1 -35 range2023 1 15 20 annotation2022 1 -10 range2022 1 38 43 annotation2024 1 OR1 range2024 1 25 41 annotation2025 1 OR2 range2025 1 1 17 BBa_K325210 1 BBa_K325210 Red Firefly Luciferase and LRE<BR><i>L. Cruciata<BR>(E. coli optimised)</i> 2010-09-14T11:00:00Z 2015-05-08T01:12:02Z This was codon-optimised for E.coli and syntesised by DNA 2.0. Released HQ 2013 This part is contains the coding sequence for a mutant light-emitting enzyme (luciferase) and luciferin regenerating enzyme (LRE) from the Japanese firefly, Luciola cruciata. It will make bacteria emit light at a reddish wavelength when placed under a promoter, provided the media is supplemented with luciferin. The luciferin regenerating enzyme will convert oxyluciferin into CHBT. CHBT is converted to more luciferin in the presence of D-cysteine. false false _446_ 0 7466 9 In stock false Codon optimisation, making compatible with BioBrick assembly format. false Hannah Copley and Theo Sanderson annotation2080150 1 Luciferin regenerating enzyme (L. cruciata) range2080150 1 18 944 annotation2080149 1 rbs range2080149 1 1 17 annotation2080152 1 BamH1 site range2080152 1 951 956 annotation2080155 1 double stop codon range2080155 1 2618 2623 annotation2080151 1 double stop codon range2080151 1 945 950 annotation2080154 1 Red luciferase (286) (L. cruciata) range2080154 1 974 2617 annotation2103099 1 G to A: Ser286Asn range2103099 1 1830 1830 annotation2080153 1 rbs range2080153 1 957 973 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K892999 1 BBa_K892999 red light responsive inverter driving luciferase 2012-06-20T11:00:00Z 2015-05-08T01:13:41Z composite of parts-registry parts In the presence of red light, the inverter is turned off, and luciferase is expressed, producing red light. false false _1156_ 0 13850 9 Not in stock false we used standard assembly false William Conrad component2177010 1 BBa_Q04510 component2176992 1 BBa_R0082 component2177019 1 BBa_K325210 annotation2176992 1 BBa_R0082 range2176992 1 1 108 annotation2177019 1 BBa_K325210 range2177019 1 1112 3734 annotation2177010 1 BBa_Q04510 range2177010 1 117 1103 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K892999_sequence 1 tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagttaaggaggtaaaaaaaatggccccgaccgtggaacaaattgtggagctgggtacgtatctgctggccgaaagcccgcactgggacgatgaaacgcaaagcctgtatttcgtcgatatcgttggtcgcagcgtgaacaaatacgttccgacgaccaagacgcatacccaactgaaattcgacaagaatccgagcttcatcatcccggtgaagggttgttccgaccgttttatcgtcagcctggaacgcgagatcaacctgctgacctgggacggcgcatcctctgcaccatccaagattgagaagatcgcggttttcgataatacgccagagaagagcgaaaaccgtctgaacgatggtaaggcagatccgctgggcaacctgtgggcgggtaccatgaatatgggttctgaccataccaccggcacgccggtgcgcggtaccctgagcagcctgagcaacaagcaagtcaaagagcacgtcagcgaggtctgcatcagcaatggcctggcatggagcaaggacctgaagaaattctattacattgatagcgctgttcgtcaagttgaccagttcgatttcgatgcgaaaaacctgagcttgagcaatcgtcaaccgctgttcacctttgacaaacacggcatcatgggtagcccggatggtcagaccatcgatgcggagggtaacctgtgggttgccacctgtcagggcgataaagtcctgaagattgatacttctaccccggaaaccctgctgggtattgtggagattccggaacatcaggtgacgagcgtttgcatcggcggtgcagaactgaacgttttgtatgtgacgaccgcgtccattaaactgcctggtgcggatgaaaccaagccgatgaaaggcgccatctataaagtcaccggtctgggtgtcaaaggtctgccgggtgaccgtgttaagctgtaataaggatccttaaggaggtaaaaaaaatggagaacatggagaacgacgagaatattgtggttggtccgaaaccgttctacccgatcgaagagggcagcgcgggtacccagctgcgtaaatacatggaacgctacgcgaaactgggtgcgatcgcgttcacgaatgcggttaccggcgtggactattcctatgcggagtatttggaaaagtcgtgttgtctgggcaaggcgttacagaattatggcctggtggtcgatggccgtatcgcgctgtgcagcgagaactgtgaagagtttttcattccggtgatcgcgggtttgtttattggcgtgggcgttgcaccgaccaatgaaatctacacgctgcgcgaactggtgcactccctgggtatctctaagccgacgatcgttttcagctccaagaaaggtttggataaagttattacggtgcaaaagacggtcaccacgatcaaaacgattgtgatcctggacagcaaagttgattaccgcggttatcaatgcctggatacgttcatcaagcgtaacaccccgccaggttttcaagcatctagcttcaaaacggtggaggttgaccgtaaagaacaagtcgcactgattatgaatagctcgggtagcacgggcctgccaaagggtgtgcagctgacccatgaaaacaccgttacccgttttagccacgcgcgtgatccgatttacggcaatcaagtgtcgccgggtacggctgttctgactgttgtcccttttcaccatggttttggcatgtttaccactctgggttacctgatttgcggttttcgcgtcgttatgctgaccaagtttgatgaagaaaccttcctgaaaaccctgcaagactacaagtgcactaatgtgattctggtcccgacgctgttcgcgatcctgaacaaaagcgagctgctgaataagtacgacctgagcaatttggtggagattgcgagcggcggtgctccgttgagcaaagaagtaggcgaggcagtggcacgtcgctttaacctgcctggcgtccgtcaaggttacggtttgactgaaaccacgagcgcgattatcatcaccccggaaggtgacgataaacctggcgctagcggtaaggtcgtgccgttgttcaaggctaaagttattgatctggacaccaagaagagcctgggcccgaatcgccgtggtgaagtttgcgttaagggcccgatgctgatgaaaggttatgtaaacaatccggaagcaacgaaagagctgatcgacgaagagggctggctgcacactggcgatatcggctactacgatgaagagaaacactttttcattgttgatcgcctgaaaagcctgatcaagtacaagggttaccaagtcccgccagcggagctggaaagcgtactgttgcagcacccgtcgattttcgacgccggtgtggctggtgtgcctgatccggtggctggcgaactgccgggtgctgtcgtggtgctggagagcggtaagaatatgaccgaaaaagaagtcatggactatgtggcgagccaagtgtccaatgcaaaacgtctgcgtggcggtgttcgctttgtggacgaagtgccgaaaggtctgaccggcaaaattgacggtcgtgcaattcgtgagattctgaagaaaccggtcgcgaaaatgtaataa BBa_C0051_sequence 1 atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc BBa_R0082_sequence 1 tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact BBa_Q04510_sequence 1 aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgc BBa_B0034_sequence 1 aaagaggagaaa BBa_R0051_sequence 1 taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc BBa_K325210_sequence 1 ttaaggaggtaaaaaaaatggccccgaccgtggaacaaattgtggagctgggtacgtatctgctggccgaaagcccgcactgggacgatgaaacgcaaagcctgtatttcgtcgatatcgttggtcgcagcgtgaacaaatacgttccgacgaccaagacgcatacccaactgaaattcgacaagaatccgagcttcatcatcccggtgaagggttgttccgaccgttttatcgtcagcctggaacgcgagatcaacctgctgacctgggacggcgcatcctctgcaccatccaagattgagaagatcgcggttttcgataatacgccagagaagagcgaaaaccgtctgaacgatggtaaggcagatccgctgggcaacctgtgggcgggtaccatgaatatgggttctgaccataccaccggcacgccggtgcgcggtaccctgagcagcctgagcaacaagcaagtcaaagagcacgtcagcgaggtctgcatcagcaatggcctggcatggagcaaggacctgaagaaattctattacattgatagcgctgttcgtcaagttgaccagttcgatttcgatgcgaaaaacctgagcttgagcaatcgtcaaccgctgttcacctttgacaaacacggcatcatgggtagcccggatggtcagaccatcgatgcggagggtaacctgtgggttgccacctgtcagggcgataaagtcctgaagattgatacttctaccccggaaaccctgctgggtattgtggagattccggaacatcaggtgacgagcgtttgcatcggcggtgcagaactgaacgttttgtatgtgacgaccgcgtccattaaactgcctggtgcggatgaaaccaagccgatgaaaggcgccatctataaagtcaccggtctgggtgtcaaaggtctgccgggtgaccgtgttaagctgtaataaggatccttaaggaggtaaaaaaaatggagaacatggagaacgacgagaatattgtggttggtccgaaaccgttctacccgatcgaagagggcagcgcgggtacccagctgcgtaaatacatggaacgctacgcgaaactgggtgcgatcgcgttcacgaatgcggttaccggcgtggactattcctatgcggagtatttggaaaagtcgtgttgtctgggcaaggcgttacagaattatggcctggtggtcgatggccgtatcgcgctgtgcagcgagaactgtgaagagtttttcattccggtgatcgcgggtttgtttattggcgtgggcgttgcaccgaccaatgaaatctacacgctgcgcgaactggtgcactccctgggtatctctaagccgacgatcgttttcagctccaagaaaggtttggataaagttattacggtgcaaaagacggtcaccacgatcaaaacgattgtgatcctggacagcaaagttgattaccgcggttatcaatgcctggatacgttcatcaagcgtaacaccccgccaggttttcaagcatctagcttcaaaacggtggaggttgaccgtaaagaacaagtcgcactgattatgaatagctcgggtagcacgggcctgccaaagggtgtgcagctgacccatgaaaacaccgttacccgttttagccacgcgcgtgatccgatttacggcaatcaagtgtcgccgggtacggctgttctgactgttgtcccttttcaccatggttttggcatgtttaccactctgggttacctgatttgcggttttcgcgtcgttatgctgaccaagtttgatgaagaaaccttcctgaaaaccctgcaagactacaagtgcactaatgtgattctggtcccgacgctgttcgcgatcctgaacaaaagcgagctgctgaataagtacgacctgagcaatttggtggagattgcgagcggcggtgctccgttgagcaaagaagtaggcgaggcagtggcacgtcgctttaacctgcctggcgtccgtcaaggttacggtttgactgaaaccacgagcgcgattatcatcaccccggaaggtgacgataaacctggcgctagcggtaaggtcgtgccgttgttcaaggctaaagttattgatctggacaccaagaagagcctgggcccgaatcgccgtggtgaagtttgcgttaagggcccgatgctgatgaaaggttatgtaaacaatccggaagcaacgaaagagctgatcgacgaagagggctggctgcacactggcgatatcggctactacgatgaagagaaacactttttcattgttgatcgcctgaaaagcctgatcaagtacaagggttaccaagtcccgccagcggagctggaaagcgtactgttgcagcacccgtcgattttcgacgccggtgtggctggtgtgcctgatccggtggctggcgaactgccgggtgctgtcgtggtgctggagagcggtaagaatatgaccgaaaaagaagtcatggactatgtggcgagccaagtgtccaatgcaaaacgtctgcgtggcggtgttcgctttgtggacgaagtgccgaaaggtctgaccggcaaaattgacggtcgtgcaattcgtgagattctgaagaaaccggtcgcgaaaatgtaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z