BBa_K895003 1 BBa_K895003 Salty_TtrRS_two_component_system 2012-09-21T11:00:00Z 2015-05-08T01:13:41Z PCR product using <i>Salmonella</i> LT2 genomic DNA as template. This is a signalling system comprising two open reading frames of the <i>ttrR</i> and <i>ttrS</i> genes together with the promoter/operator of the <i>ttrB</i> gene from <i>Salmonella</i> LT2. Together, TtrR and TtrS form a canonical two-component system where TtrS is the membrane-bound sensor kinase and TtrR is the DNA-binding response regulator. The system senses tetrathionate outside the cell and activates transcription of the <i>ttrB</i> gene, which is part of a tetrathionate reducatase. This part has been designed so that expression of alternative reporter genes can be regulated by tetrathionate. false false _1160_ 0 8083 9 It's complicated false A PstI site in the natural <i>ttrS</i> sequence has been silently removed. false Frank Sargent annotation2194071 1 promotor for tetrathionate regulon range2194071 1 2351 2555 annotation2194070 1 -35 Consensus range2194070 1 2438 2443 annotation2194048 1 ttrS range2194048 1 596 2275 annotation2194052 1 -10 Consensus range2194052 1 2462 2468 annotation2194047 1 ttrR range2194047 1 1 621 annotation2194049 1 fnr binding-site range2194049 1 2397 2410 annotation2194050 1 RBS range2194050 1 2542 2548 annotation2194072 1 BamHI site range2194072 1 2550 2555 annotation2194051 1 transcription start range2194051 1 2475 2475 BBa_K895003_sequence 1 tcatggctcatacgttgttcgtattctggtctctggcgaggccattttttcgaaacgcctaatcagttccgccaggctaccggcctgcattttttccatgactctggcgcggtgcacctctacggtacgcaccgcgatattcatcgcttccgcaatttcacggttcataaatccttttgccaccaggctggccagctcacgctctttcggcgtcaactgctggtaacacagtataatctcacgacgcgccaccgctgccgatgaaaccgtcagcgcacgctccagcgccgcctgtagcggttttaccgataccggtttttgcagaaaatcgacggcgccgcgtttcatctgctccacggccatcggtacatcgccatgcccggtaagaaaaacaaccgccagggtacttccgcactggcgcaacgcatcatgaacgccctgcccatccagtaccggcattcgcatatccagtaatacgaccccggcctgatacagactggcctgcgccaaaaaatccgccccctgcgtccagcattttacgtcatatcccagactttccagtaaaaacgcgcacgcgttagtgaccgccgtatcatcatccagtagatgaattgtcgccatccctgcccccattttcatgtaagaaatgtatcgtaaccaccgttcccgacagaccgtccggcgcggtctggttcctgatgctgatatcgccccgcccataccgcaccagccgctggcaaatcgccagccctaagcccatcccctctttacgggtggtcataaacggctgaaacgcctgacgtaatagcgcctcatcgattcccccggcgttatcctgtaaaacaatactgatgccgttttcagtgcgttcagcaacgatccataaatgggtggcgcccgcctgagccgcattaagaatgatattcgccagcacctgttccagcagcactgacggcagcgttacgcgcagcgcagcgctaacctcggtatgcagagtcactgtcggaaactgttgcgccatacgcaacaattgccagacatgatcaatcgcctcgcgaatggctatggccttccacgcttcggttagcaccgggttgccctgcgcctggctgacccagtgacgcaggttacgcagagtatccgcaccgcgttgcgcctgctggtcaatctgctccagcgccggcagcaagggatgctgttcatctgccgcgcgcagtcgaatcaggcacccctgggcataatgtcgaatcgcggaaagcggctgattaagctcatgggcaaacccggaggtcatttcacccaacacgctcatttgccgggcggtttccagcgcccgctcatgctgatgaagaactacgctattacgttccagttgctttccacgtcgacgcaccagcagcatgacccaaatataattgagcgtgagcaacaagaacgccagaatcacgccgccgaccattagctggtgctggattaaccaacttttgacatccagccacagtcgacgctgctgagggtgctgacgaacatcacgcagcaaggcttccacctgactggtggacgcaggcgcgccccagtgaaatgacgcggcggcgggcgcgttgaatagcgctcgcgttacgcgatccgccagcgcatcgcttaccgcaggtagcgccgcgaacgaccagtcaggatataacggcgtactggttaagcaaggcaggggcgtcggtcgggaaagcagcgcgataaagtcctttttattaatcaatccttcctgatccatattttctaacaggcacactggcacaattgccgcctgcaccgctttttcgcgcagcatatagactaaggcatcgccaggaaatccggtaaaacggagatgaaaatcgcgctccgggcgtaagcccgcgtcgctgagcgctttatagcctaataaatagccgccaaacgcctgagcatcaatcgcgccgacggtcttaccgatgagatcatgcgccgtggtgatgccgctatcgcgccgggtcaaaatcacgctgccaataacattactcaccgctttcccatcgcgcgtggagcgcagggaagctaaccagcgcagcggcgcatggctgttcagttggacaaattgcgccgggttggttatcacaaactgcacggttccctggttaacggcctcctgcatttgatgcagatccagcggctggatgtgaaaggtttcgcctggaagctgttggcttaatgtctttgccaacggttgccagtggctacgcgtagacgcctcgccgcgcatggccaaaataccgatattccacgtccctgcccacgcgccatgacaaagtagccctactgccgccaacaccgccaggcgccttacggttttacctctcaccccaatatccctgtcaattatgttgttttagatcaacaacaagccgggtatgtggttaaccacaatagagcgcaccccgcctcgatttttacactgtaaatcatcgacattttttattcattacacatgaaccaacatcgtgacaaatgtttcattgttggcaatgtggacgggaggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z