BBa_K895007 1 BBa_K895007 Tetrathionate-sensing two-component system linked to GFP production. 2012-09-22T11:00:00Z 2015-05-08T01:13:41Z This a combination of <partinfo>BBa_K895003</partinfo> and <partinfo>BBa_K895006</partinfo> cloned together by PCR. This is a biosensor comprising the engineered <i>Salmonella</i> TtrRS twon-component system from <partinfo>BBa_K895003</partinfo> linked to GFP from <partinfo>BBa_K895006</partinfo>, which was originally supplied in <partinfo>BBa_J54103</partinfo>. The concept is that, primarily under anaerobic conditions, the two-component system will sense tetrathionate and induce GFP production. false false _1160_ 0 8083 9 It's complicated true The GFP ATG is positioned immediately downstream of an engineered BamHI site. This places the start codon at exactly the correct distance from the <i>ttrB</i> RBS. false Frank Sargent annotation2203416 1 GFP range2203416 1 2556 3275 annotation2203415 1 BamHI site range2203415 1 2550 2555 annotation2203410 1 ttrR range2203410 1 1 621 annotation2203411 1 BBa_K895007 range2203411 1 1 3275 annotation2203413 1 ttrB promoter region range2203413 1 2351 2555 annotation2203414 1 RBS range2203414 1 2542 2548 annotation2203412 1 ttrS range2203412 1 596 2275 BBa_K895007_sequence 1 tcatggctcatacgttgttcgtattctggtctctggcgaggccattttttcgaaacgcctaatcagttccgccaggctaccggcctgcattttttccatgactctggcgcggtgcacctctacggtacgcaccgcgatattcatcgcttccgcaatttcacggttcataaatccttttgccaccaggctggccagctcacgctctttcggcgtcaactgctggtaacacagtataatctcacgacgcgccaccgctgccgatgaaaccgtcagcgcacgctccagcgccgcctgtagcggttttaccgataccggtttttgcagaaaatcgacggcgccgcgtttcatctgctccacggccatcggtacatcgccatgcccggtaagaaaaacaaccgccagggtacttccgcactggcgcaacgcatcatgaacgccctgcccatccagtaccggcattcgcatatccagtaatacgaccccggcctgatacagactggcctgcgccaaaaaatccgccccctgcgtccagcattttacgtcatatcccagactttccagtaaaaacgcgcacgcgttagtgaccgccgtatcatcatccagtagatgaattgtcgccatccctgcccccattttcatgtaagaaatgtatcgtaaccaccgttcccgacagaccgtccggcgcggtctggttcctgatgctgatatcgccccgcccataccgcaccagccgctggcaaatcgccagccctaagcccatcccctctttacgggtggtcataaacggctgaaacgcctgacgtaatagcgcctcatcgattcccccggcgttatcctgtaaaacaatactgatgccgttttcagtgcgttcagcaacgatccataaatgggtggcgcccgcctgagccgcattaagaatgatattcgccagcacctgttccagcagcactgacggcagcgttacgcgcagcgcagcgctaacctcggtatgcagagtcactgtcggaaactgttgcgccatacgcaacaattgccagacatgatcaatcgcctcgcgaatggctatggccttccacgcttcggttagcaccgggttgccctgcgcctggctgacccagtgacgcaggttacgcagagtatccgcaccgcgttgcgcctgctggtcaatctgctccagcgccggcagcaagggatgctgttcatctgccgcgcgcagtcgaatcaggcacccctgggcataatgtcgaatcgcggaaagcggctgattaagctcatgggcaaacccggaggtcatttcacccaacacgctcatttgccgggcggtttccagcgcccgctcatgctgatgaagaactacgctattacgttccagttgctttccacgtcgacgcaccagcagcatgacccaaatataattgagcgtgagcaacaagaacgccagaatcacgccgccgaccattagctggtgctggattaaccaacttttgacatccagccacagtcgacgctgctgagggtgctgacgaacatcacgcagcaaggcttccacctgactggtggacgcaggcgcgccccagtgaaatgacgcggcggcgggcgcgttgaatagcgctcgcgttacgcgatccgccagcgcatcgcttaccgcaggtagcgccgcgaacgaccagtcaggatataacggcgtactggttaagcaaggcaggggcgtcggtcgggaaagcagcgcgataaagtcctttttattaatcaatccttcctgatccatattttctaacaggcacactggcacaattgccgcctgcaccgctttttcgcgcagcatatagactaaggcatcgccaggaaatccggtaaaacggagatgaaaatcgcgctccgggcgtaagcccgcgtcgctgagcgctttatagcctaataaatagccgccaaacgcctgagcatcaatcgcgccgacggtcttaccgatgagatcatgcgccgtggtgatgccgctatcgcgccgggtcaaaatcacgctgccaataacattactcaccgctttcccatcgcgcgtggagcgcagggaagctaaccagcgcagcggcgcatggctgttcagttggacaaattgcgccgggttggttatcacaaactgcacggttccctggttaacggcctcctgcatttgatgcagatccagcggctggatgtgaaaggtttcgcctggaagctgttggcttaatgtctttgccaacggttgccagtggctacgcgtagacgcctcgccgcgcatggccaaaataccgatattccacgtccctgcccacgcgccatgacaaagtagccctactgccgccaacaccgccaggcgccttacggttttacctctcaccccaatatccctgtcaattatgttgttttagatcaacaacaagccgggtatgtggttaaccacaatagagcgcaccccgcctcgatttttacactgtaaatcatcgacattttttattcattacacatgaaccaacatcgtgacaaatgtttcattgttggcaatgtggacgggaggatccatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z