LOCUS       BBa_K896914             1121 bp    DNA     linear       26-Nov-2024
DEFINITION  composite
ACCESSION   BBa_K896914
VERSION     BBa_K896914.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..78
                     /label=BBa_I742156
     CDS             85..984
                     /label=BBa_K896913
     stem_loop       1004..1047
                     /label=stem_loop
     misc_feature    993..1072
                     /label=BBa_B0010
     terminator      993..1072
                     /label=BBa_B0010
     stem_loop       1088..1107
                     /label=T7 TE
     misc_feature    1114..1114
                     /label=stop
     polyA_site      1108..1121
                     /label=polya
     misc_feature    1081..1121
                     /label=BBa_B0012
     terminator      1081..1121
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agctctaccg gagaaattta ctagatgaac gtcaactcga accttgcgca gctatcagat
      121 atggctttcg catcgagctt cgttatttac ttcatcgcgt tggtgttatc catcgtgtac
      181 tacatgaagg ttaagggaat cgttgatcag cagcgtgaag gcctagagcc ctccacggca
      241 tcggcggata agttcgctgg tatgacgcag atgattgtgt ggcttggtgt tttcgtccac
      301 attgcttccg tggtgttgcg tggtttgtcg gcgagccgat tccctttcgg aaacctctat
      361 gagtacatct tggtgatcag cgcttttgcc gttgctgtgg ctgctggttt cttgcagcgc
      421 catgagcttc gggctatgtg gccatgggtg cttaccccag tgctggcctt gctgttttat
      481 ggtggcacca agctgtatgc ggattccgct ccggtggttc ctgctttgca gtccttctgg
      541 ttcccgattc acgtatcgac ggtttccatt ggcgcgtcga ttggtttgat ttctggggtg
      601 gcgtccttgt tgtatctgct gcgtatgagc aagaacaagc gtcttgcggc tttaacgtcg
      661 ccgttgcctt ctgctaagac gttggatgcg attgcttacc gtacggctat tgtgactgtg
      721 ccagtttttg gtttgggtat tgttcttggt gctatttggg cggagagcgc gtggggtcgt
      781 ttctggggtt gggaccctaa ggagactgtc gcgtttatta cttggatttt gtatgcggct
      841 tatctgcatg ctcgtgctac cgccgggtgg cgtgacacca aggctgcatg gatcaatatc
      901 gtggcattga taaccatggt gtttaacttg ttctttatta atattgtggt gtcaggcctg
      961 cactcttatg ctggcttgaa ttagtactag agccaggcat caaataaaac gaaaggctca
     1021 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1081 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//