BBa_K896981
1
BBa_K896981
short description
2012-04-25T11:00:00Z
2015-05-08T01:13:42Z
source of this part
long description
false
false
_1161_
0
12744
9
Not in stock
false
Enter any design considerations you had to deal with during the detailed design of the sequence.
false
Chun-Ying Chou
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_J23101
1
BBa_J23101
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
later
Released HQ 2013
later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K896982
1
BBa_K896982
short description
2012-04-25T11:00:00Z
2015-05-08T01:13:42Z
source
long description
false
false
_1161_
0
12744
9
Not in stock
false
enter any design considerations you had to deal with during the detailed design of the sequence
false
Chun-Ying Chou
component2223087
1
BBa_B0034
component2223088
1
BBa_K896981
component2223085
1
BBa_J23101
component2223091
1
BBa_B0012
component2223089
1
BBa_B0010
annotation2223088
1
BBa_K896981
range2223088
1
62
325
annotation2223091
1
BBa_B0012
range2223091
1
422
462
annotation2223087
1
BBa_B0034
range2223087
1
44
55
annotation2223085
1
BBa_J23101
range2223085
1
1
35
annotation2223089
1
BBa_B0010
range2223089
1
334
413
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K896982_sequence
1
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgattgatgccgatgatctttgcaaagatgctgattctttgagggctgatgttatcgccaggatcattggacgtctgggtcttgagaagaccatgtttgaagcatcaaataagcaaactactgaatggtttatcaaacgatatggccccaaggtaaacctctttgtggatcattctcaagtaattggcgtggagaacctccgaggctgcaacacaggtacaaatatttctcgtgagccaggatcctcatatctcctcttctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K896981_sequence
1
atgattgatgccgatgatctttgcaaagatgctgattctttgagggctgatgttatcgccaggatcattggacgtctgggtcttgagaagaccatgtttgaagcatcaaataagcaaactactgaatggtttatcaaacgatatggccccaaggtaaacctctttgtggatcattctcaagtaattggcgtggagaacctccgaggctgcaacacaggtacaaatatttctcgtgagccaggatcctcatatctcctcttctga
BBa_J23101_sequence
1
tttacagctagctcagtcctaggtattatgctagc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z