BBa_K896981 1 BBa_K896981 short description 2012-04-25T11:00:00Z 2015-05-08T01:13:42Z source of this part long description false false _1161_ 0 12744 9 Not in stock false Enter any design considerations you had to deal with during the detailed design of the sequence. false Chun-Ying Chou BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K896982 1 BBa_K896982 short description 2012-04-25T11:00:00Z 2015-05-08T01:13:42Z source long description false false _1161_ 0 12744 9 Not in stock false enter any design considerations you had to deal with during the detailed design of the sequence false Chun-Ying Chou component2223087 1 BBa_B0034 component2223088 1 BBa_K896981 component2223085 1 BBa_J23101 component2223091 1 BBa_B0012 component2223089 1 BBa_B0010 annotation2223088 1 BBa_K896981 range2223088 1 62 325 annotation2223091 1 BBa_B0012 range2223091 1 422 462 annotation2223087 1 BBa_B0034 range2223087 1 44 55 annotation2223085 1 BBa_J23101 range2223085 1 1 35 annotation2223089 1 BBa_B0010 range2223089 1 334 413 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K896982_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgattgatgccgatgatctttgcaaagatgctgattctttgagggctgatgttatcgccaggatcattggacgtctgggtcttgagaagaccatgtttgaagcatcaaataagcaaactactgaatggtttatcaaacgatatggccccaaggtaaacctctttgtggatcattctcaagtaattggcgtggagaacctccgaggctgcaacacaggtacaaatatttctcgtgagccaggatcctcatatctcctcttctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K896981_sequence 1 atgattgatgccgatgatctttgcaaagatgctgattctttgagggctgatgttatcgccaggatcattggacgtctgggtcttgagaagaccatgtttgaagcatcaaataagcaaactactgaatggtttatcaaacgatatggccccaaggtaaacctctttgtggatcattctcaagtaattggcgtggagaacctccgaggctgcaacacaggtacaaatatttctcgtgagccaggatcctcatatctcctcttctga BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z