BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K896918 1 BBa_K896918 Mediate Cellular Resistance 2012-04-26T11:00:00Z 2015-05-08T01:13:42Z RHNTHN SDFDSGBHB false false _1161_ 0 12742 9 Not in stock false EFGRBBY false Hsin-Hui Ling BBa_K896991 1 BBa_K896991 Mediate Cellular Resistance 2012-04-26T11:00:00Z 2015-05-08T01:13:42Z WEFV WEFVWEF V false false _1161_ 0 12742 9 Not in stock false WERVE false Hsin-Hui Ling component2174387 1 BBa_B0012 component2174384 1 BBa_K896918 component2174381 1 BBa_I712004 component2174385 1 BBa_B0010 component2174383 1 BBa_B0034 annotation2174387 1 BBa_B0012 range2174387 1 1160 1200 annotation2174385 1 BBa_B0010 range2174385 1 1072 1151 annotation2174381 1 BBa_I712004 range2174381 1 1 654 annotation2174384 1 BBa_K896918 range2174384 1 681 1063 annotation2174383 1 BBa_B0034 range2174383 1 663 674 BBa_I712004 1 BBa_I712004 CMV promoter 2007-10-18T11:00:00Z 2015-08-31T04:07:45Z pcDNA3 by Invitrogen a constitutive expression promoter or use in mammalian cells. Ribosome binding site is included. false false _130_ 0 1896 9 It's complicated false Cloned into pSB1AC3 vector. false Andrej Ondracka BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K896991_sequence 1 cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaattactagagaaagaggagaaatactagatgatcaagagccagcacgagatggacgggctgggggctccccagacctcagcccccgtggccaccacggtgatcaccatccccagggagacctccgtgcccgaccacatcgtctggtccctgttcaacaccctcttcctgaactggtgctgcctgggcttcgtggctttcgcctactccgtgaaggcgagggaccggaagatggtgggagacatcattggggcccagagctatgcctccaccgccaagtgcctgaacatctgggctctgatcctgggcctcattctgaccatcggagccactgttcttctggtgtttgtatacataacagcctaccatatgttagagcgcgcaaagagtaacagaggctactagaagcttgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_K896918_sequence 1 atgatcaagagccagcacgagatggacgggctgggggctccccagacctcagcccccgtggccaccacggtgatcaccatccccagggagacctccgtgcccgaccacatcgtctggtccctgttcaacaccctcttcctgaactggtgctgcctgggcttcgtggctttcgcctactccgtgaaggcgagggaccggaagatggtgggagacatcattggggcccagagctatgcctccaccgccaagtgcctgaacatctgggctctgatcctgggcctcattctgaccatcggagccactgttcttctggtgtttgtatacataacagcctaccatatgttagagcgcgcaaagagtaacagaggctactagaagcttgc BBa_I712004_sequence 1 cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaat BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z