LOCUS       BBa_K899002             2983 bp    DNA     linear       22-Nov-2024
DEFINITION  Reporter system for SST (BBa_K899000)
ACCESSION   BBa_K899002
VERSION     BBa_K899002.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    207..209
                     /label=Start codon
     misc_feature    2097..2099
                     /label=Stop codon
     CDS             207..2099
                     /label=BBa_K899000
     RBS             2108..2120
                     /label=RBS-3\Weak
     RBS             2114..2117
                     /label=RBS
     misc_feature    2108..2120
                     /label=BBa_B0032
     RBS             2108..2120
                     /label=BBa_B0032
     CDS             2127..2846
                     /label=GFP protein
     CDS             2127..2846
                     /label=BBa_E0040
     stem_loop       2866..2909
                     /label=stem_loop
     misc_feature    2855..2934
                     /label=BBa_B0010
     terminator      2855..2934
                     /label=BBa_B0010
     stem_loop       2950..2969
                     /label=T7 TE
     misc_feature    2976..2976
                     /label=stop
     polyA_site      2970..2983
                     /label=polya
     misc_feature    2943..2983
                     /label=BBa_B0012
     terminator      2943..2983
                     /label=BBa_B0012
     misc_feature    2108..2983
                     /label=BBa_E0240
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagatga tggcttcatc caccaccacc acccctctca
      241 ttctccatga tgaccctgaa aacctcccag aactcaccgg ttctccgaca actcgtcgtc
      301 tatccatcgc aaaagtgctt tcggggatcc ttgtttcggt tctggttata ggtgctcttg
      361 ttgctttaat caacaaccaa acatatgaat ccccctcggc caccacattc gtaactcagt
      421 tgccaaatat tgatctgaag cgggttccag gaaagttgga ttcgagtgct gaggttgaat
      481 ggcaacgatc cacttatcat tttcaacccg acaaaaattt cattagcgat cctgatggcc
      541 caatgtatca catgggatgg tatcatctat tttatcagta caaccctcaa tctgccatct
      601 ggggcaacat cacatggggc cactcggtat cgaaagacat gatcaactgg ttccatctcc
      661 ctttcgccat ggttcctgac cattggtacg acatcgaagg tgtcatgacg ggttcggcta
      721 cagtcctccc taatggtcaa atcatcatgc tttactcggg caacgcgtat gatctctccc
      781 aagtacaatg cttggcgtac gctgtcaact cgtcggatcc acttcttata gagtggaaaa
      841 aatatgaagg taaccctgtc ttactcccac caccaggagt aggctacaag gactttcggg
      901 acccatccac attgtggtcg ggccctgatg gtgaatatag aatggtaatg gggtccaagc
      961 acaacgagac tattggctgt gctttgattt accataccac taattttacg cattttgaat
     1021 tgaaagagga ggtgcttcat gcagtcccac atactggtat gtgggaatgt gttgatcttt
     1081 acccggtgtc caccgtacac acaaacgggc tggacatggt ggataacggg ccaaatgtta
     1141 agtacgtgtt gaaacaaagt ggggatgaag atcgccatga ttggtatgca attggaagtt
     1201 acgatatagt gaatgataag tggtacccag atgacccgga aaatgatgtg ggtatcggat
     1261 taagatatga ttttggaaaa ttttatgcgt ccaagacgtt ttatgaccaa cataagaaga
     1321 ggagagtcct ttggggctat gttggagaaa ccgatcccca aaagtatgac ctttcaaagg
     1381 gatgggctaa cattttgaat attccaagga ccgtcgtttt ggacctcgaa actaaaacca
     1441 atttgattca atggccaatc gaggaaaccg aaaaccttag gtcgaaaaag tatgatgaat
     1501 ttaaagacgt cgagcttcga cccggggcac tcgttcccct tgagataggc acagccacac
     1561 agttggatat agttgcgaca ttcgaaatcg accaaaagat gttggaatca acgctagagg
     1621 ccgatgttct attcaattgc acgacaagtg aaggctcggt tgcaaggagt gtgttgggac
     1681 cgtttggtgt ggtggttcta gccgatgccc agcgctccga acaacttcct gtatacttct
     1741 atatcgcaaa agatattgat ggaacctcac gaacttattt ttgtgccgac gaaacaagat
     1801 catccaagga tgtaagcgta gggaaatggg tgtacggaag cagtgttcct gtcctcccag
     1861 gcgaaaagta caatatgagg ttattggtgg atcattcgat agtagaggga tttgcacaaa
     1921 acgggagaac cgtggtgaca tcaagagtgt atccaacaaa ggcgatctac aacgctgcga
     1981 aggtgttttt gttcaacaac gcgactggaa tcagtgtgaa ggcgtcgatc aagatctgga
     2041 agatggggga agcagaactc aatcctttcc ctcttcctgg gtggactttc gaactttgat
     2101 actagagtca cacaggaaag tactagatgc gtaaaggaga agaacttttc actggagttg
     2161 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
     2221 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
     2281 aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg
     2341 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
     2401 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag
     2461 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
     2521 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca
     2581 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg
     2641 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
     2701 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
     2761 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
     2821 gcatggatga actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct
     2881 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     2941 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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