LOCUS BBa_K899004 4879 bp DNA linear 17-Dec-2024 DEFINITION Reporter system for SST and FFT (BBa_K899000 and BBa_K899001) ACCESSION BBa_K899004 VERSION BBa_K899004.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 209..215 /label=Shine-Dalgarno sequence misc_feature 218..221 /label=Kozak sequence RBS 209..221 /label=BBa_K899010 misc_feature 228..230 /label=Start codon misc_feature 2118..2120 /label=Stop codon CDS 228..2120 /label=BBa_K899000 misc_feature 2138..2141 /label=Kozak sequence misc_feature 2129..2135 /label=Shine-Dalgarno sequence RBS 2129..2141 /label=BBa_K899009 misc_feature 2148..2150 /label=Start codon misc_feature 3993..3995 /label=Stop codon CDS 2148..3995 /label=BBa_K899001 RBS 4004..4016 /label=RBS-3\Weak RBS 4010..4013 /label=RBS misc_feature 4004..4016 /label=BBa_B0032 RBS 4004..4016 /label=BBa_B0032 CDS 4023..4742 /label=GFP protein CDS 4023..4742 /label=BBa_E0040 stem_loop 4762..4805 /label=stem_loop misc_feature 4751..4830 /label=BBa_B0010 terminator 4751..4830 /label=BBa_B0010 stem_loop 4846..4865 /label=T7 TE misc_feature 4872..4872 /label=stop polyA_site 4866..4879 /label=polya misc_feature 4839..4879 /label=BBa_B0012 terminator 4839..4879 /label=BBa_B0012 misc_feature 4004..4879 /label=BBa_E0240 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagag gaggttacac ctactagatg atggcttcat 241 ccaccaccac cacccctctc attctccatg atgaccctga aaacctccca gaactcaccg 301 gttctccgac aactcgtcgt ctatccatcg caaaagtgct ttcggggatc cttgtttcgg 361 ttctggttat aggtgctctt gttgctttaa tcaacaacca aacatatgaa tccccctcgg 421 ccaccacatt cgtaactcag ttgccaaata ttgatctgaa gcgggttcca ggaaagttgg 481 attcgagtgc tgaggttgaa tggcaacgat ccacttatca ttttcaaccc gacaaaaatt 541 tcattagcga tcctgatggc ccaatgtatc acatgggatg gtatcatcta ttttatcagt 601 acaaccctca atctgccatc tggggcaaca tcacatgggg ccactcggta tcgaaagaca 661 tgatcaactg gttccatctc cctttcgcca tggttcctga ccattggtac gacatcgaag 721 gtgtcatgac gggttcggct acagtcctcc ctaatggtca aatcatcatg ctttactcgg 781 gcaacgcgta tgatctctcc caagtacaat gcttggcgta cgctgtcaac tcgtcggatc 841 cacttcttat agagtggaaa aaatatgaag gtaaccctgt cttactccca ccaccaggag 901 taggctacaa ggactttcgg gacccatcca cattgtggtc gggccctgat ggtgaatata 961 gaatggtaat ggggtccaag cacaacgaga ctattggctg tgctttgatt taccatacca 1021 ctaattttac gcattttgaa ttgaaagagg aggtgcttca tgcagtccca catactggta 1081 tgtgggaatg tgttgatctt tacccggtgt ccaccgtaca cacaaacggg ctggacatgg 1141 tggataacgg gccaaatgtt aagtacgtgt tgaaacaaag tggggatgaa gatcgccatg 1201 attggtatgc aattggaagt tacgatatag tgaatgataa gtggtaccca gatgacccgg 1261 aaaatgatgt gggtatcgga ttaagatatg attttggaaa attttatgcg tccaagacgt 1321 tttatgacca acataagaag aggagagtcc tttggggcta tgttggagaa accgatcccc 1381 aaaagtatga cctttcaaag ggatgggcta acattttgaa tattccaagg accgtcgttt 1441 tggacctcga aactaaaacc aatttgattc aatggccaat cgaggaaacc gaaaacctta 1501 ggtcgaaaaa gtatgatgaa tttaaagacg tcgagcttcg acccggggca ctcgttcccc 1561 ttgagatagg cacagccaca cagttggata tagttgcgac attcgaaatc gaccaaaaga 1621 tgttggaatc aacgctagag gccgatgttc tattcaattg cacgacaagt gaaggctcgg 1681 ttgcaaggag tgtgttggga ccgtttggtg tggtggttct agccgatgcc cagcgctccg 1741 aacaacttcc tgtatacttc tatatcgcaa aagatattga tggaacctca cgaacttatt 1801 tttgtgccga cgaaacaaga tcatccaagg atgtaagcgt agggaaatgg gtgtacggaa 1861 gcagtgttcc tgtcctccca ggcgaaaagt acaatatgag gttattggtg gatcattcga 1921 tagtagaggg atttgcacaa aacgggagaa ccgtggtgac atcaagagtg tatccaacaa 1981 aggcgatcta caacgctgcg aaggtgtttt tgttcaacaa cgcgactgga atcagtgtga 2041 aggcgtcgat caagatctgg aagatggggg aagcagaact caatcctttc cctcttcctg 2101 ggtggacttt cgaactttga tactagagag gaggtgtcac ctactagatg caaacccctg 2161 aaccctttac agaccttgaa catgaacccc acacacccct actggaccac caccacaacc 2221 caccaccaca aaccaccaca aaacctttgt tcaccagggt tgtgtccggt gtcacctttg 2281 ttttattctt ctttggtttc gctatcgtat tcattgttct caaccaacag aactcttctg 2341 ttcgtatcgt caccaattcg gagaaatctt ttataaggta ttcgcagacc gatcgcttgt 2401 cgtgggaacg gaccgctttt cattttcagc ctgccaagaa ttttatttac gatccagatg 2461 gtcagttgtt tcacatgggc tggtaccata tgttctatca atacaaccca tacgcaccgg 2521 tttggggcaa tatgtcatgg ggtcactcag tgtccaaaga catgatcaac tggtacgagc 2581 tgccagtcgc tatggtcccg accgaatggt atgatatcga gggcgtctta tccgggtcta 2641 ccacggtcct tccaaacggt cagatctttg cattgtatac tgggaacgct aatgattttt 2701 cccaattaca atgcaaagct gtacccgtaa acttatctga cccgcttctt attgagtggg 2761 tcaagtatga ggataaccca atcctgtaca ctccaccagg gattgggtta aaggactatc 2821 gggacccgtc aacagtctgg acaggtcccg atggaaagca taggatgatc atgggaacta 2881 aacgtggcaa tacaggcatg gtacttgttt actataccac tgattacacg aactacgagt 2941 tgttggatga gccgttgcac tctgttccca acaccgatat gtgggaatgc gtcgactttt 3001 acccggtttc gttaaccaat gatagtgcac ttgatatggc ggcctatggg tcgggtatca 3061 aacacgttat taaagaaagt tgggagggac atggaatgga ttggtattca atcgggacat 3121 atgacgcgat aaatgataaa tggactcccg ataacccgga actagatgtc ggtatcgggt 3181 tacggtgcga ttacgggagg ttttttgcat caaagagtct ttatgaccca ttgaagaaaa 3241 ggaggatcac ttggggttat gttggagaat cagatagtgc tgatcaggac ctctcaagag 3301 gatgggctac tgtttataat gttggaagaa caattgtact agatagaaag accgggaccc 3361 atttacttca ttggcccgtt gaggaagtcg agagtttgag atacaacggt caggagttta 3421 aagagatcaa gctagagccc ggttcaatca ttccactcga cataggcacg gctacacagt 3481 tggacatagt tgcaacattt gaggtggatc aagcagcgtt gaacgcgaca agtgaaaccg 3541 atgatattta tggttgcacc actagcttag gtgcagccca aaggggaagt ttgggaccat 3601 ttggtcttgc ggttctagcc gatggaaccc tttctgagtt aactccggtt tatttctata 3661 tagctaaaaa ggcagatgga ggtgtgtcga cacatttttg taccgataag ctaaggtcat 3721 cactagatta tgatggggag agagtggtgt atgggggcac tgttcctgtg ttagatgatg 3781 aagaactcac aatgaggcta ttggtggatc attcgatagt ggaggggttt gcgcaaggag 3841 gaaggacggt tataacatca agggcgtatc caacaaaagc gatatacgaa caagcgaagc 3901 tgttcttgtt caacaacgcc acaggtacga gtgtgaaagc atctctcaag atttggcaaa 3961 tggcttctgc accaattcat caataccctt tttaatacta gagtcacaca ggaaagtact 4021 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg 4081 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg 4141 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac 4201 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac 4261 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt 4321 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg 4381 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca 4441 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg 4501 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag 4561 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt 4621 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc 4681 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat 4741 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 4801 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 4861 gggcctttct gcgtttata //