##gff-version 3
##sequence-region BBa_K899004 1 4879
BBa_K899004	.	ribosome_entry_site	2129	2141	.	+	0	ID=BBa_K899009;Name=BBa_K899009
BBa_K899004	.	sequence_feature	2129	2135	.	+	0	ID=annotation2201693;Name=Shine-Dalgarno sequence;Parent=BBa_K899009
BBa_K899004	.	sequence_feature	2138	2141	.	+	0	ID=annotation2201692;Name=Kozak sequence;Parent=BBa_K899009
BBa_K899004	.	ribosome_entry_site	209	221	.	+	0	ID=BBa_K899010;Name=BBa_K899010
BBa_K899004	.	sequence_feature	218	221	.	+	0	ID=annotation2201790;Name=Kozak sequence;Parent=BBa_K899010
BBa_K899004	.	sequence_feature	209	215	.	+	0	ID=annotation2201787;Name=Shine-Dalgarno sequence;Parent=BBa_K899010
BBa_K899004	.	engineered_region	4004	4879	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K899004	.	terminator	4839	4879	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K899004	.	engineered_region	4839	4879	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K899004	.	stem_loop	4846	4865	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K899004	.	stop_codon	4872	4872	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K899004	.	polyA_site	4866	4879	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K899004	.	terminator	4751	4830	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K899004	.	engineered_region	4751	4830	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K899004	.	stem_loop	4762	4805	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K899004	.	ribosome_entry_site	4004	4016	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K899004	.	ribosome_entry_site	4004	4016	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K899004	.	ribosome_entry_site	4010	4013	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K899004	.	engineered_region	4004	4016	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K899004	.	CDS	4023	4742	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K899004	.	CDS	4023	4742	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K899004	.	CDS	228	2120	.	+	0	ID=BBa_K899000;Name=BBa_K899000
BBa_K899004	.	stop_codon	2118	2120	.	+	0	ID=annotation2193664;Name=Stop codon;Parent=BBa_K899000
BBa_K899004	.	start_codon	228	230	.	+	0	ID=annotation2193663;Name=Start codon;Parent=BBa_K899000
BBa_K899004	.	CDS	2148	3995	.	+	0	ID=BBa_K899001;Name=BBa_K899001
BBa_K899004	.	stop_codon	3993	3995	.	+	0	ID=annotation2193673;Name=Stop codon;Parent=BBa_K899001
BBa_K899004	.	start_codon	2148	2150	.	+	0	ID=annotation2193672;Name=Start codon;Parent=BBa_K899001
BBa_K899004	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K899004	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K899004	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K899004	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K899004	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K899004	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K899004	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K899004	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
>BBa_K899004
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaggaggttacacctactagatgatggctt
catccaccaccaccacccctctcattctccatgatgaccctgaaaacctcccagaactcaccggttctccgacaactcg
tcgtctatccatcgcaaaagtgctttcggggatccttgtttcggttctggttataggtgctcttgttgctttaatcaac
aaccaaacatatgaatccccctcggccaccacattcgtaactcagttgccaaatattgatctgaagcgggttccaggaa
agttggattcgagtgctgaggttgaatggcaacgatccacttatcattttcaacccgacaaaaatttcattagcgatcc
tgatggcccaatgtatcacatgggatggtatcatctattttatcagtacaaccctcaatctgccatctggggcaacatc
acatggggccactcggtatcgaaagacatgatcaactggttccatctccctttcgccatggttcctgaccattggtacg
acatcgaaggtgtcatgacgggttcggctacagtcctccctaatggtcaaatcatcatgctttactcgggcaacgcgta
tgatctctcccaagtacaatgcttggcgtacgctgtcaactcgtcggatccacttcttatagagtggaaaaaatatgaa
ggtaaccctgtcttactcccaccaccaggagtaggctacaaggactttcgggacccatccacattgtggtcgggccctg
atggtgaatatagaatggtaatggggtccaagcacaacgagactattggctgtgctttgatttaccataccactaattt
tacgcattttgaattgaaagaggaggtgcttcatgcagtcccacatactggtatgtgggaatgtgttgatctttacccg
gtgtccaccgtacacacaaacgggctggacatggtggataacgggccaaatgttaagtacgtgttgaaacaaagtgggg
atgaagatcgccatgattggtatgcaattggaagttacgatatagtgaatgataagtggtacccagatgacccggaaaa
tgatgtgggtatcggattaagatatgattttggaaaattttatgcgtccaagacgttttatgaccaacataagaagagg
agagtcctttggggctatgttggagaaaccgatccccaaaagtatgacctttcaaagggatgggctaacattttgaata
ttccaaggaccgtcgttttggacctcgaaactaaaaccaatttgattcaatggccaatcgaggaaaccgaaaaccttag
gtcgaaaaagtatgatgaatttaaagacgtcgagcttcgacccggggcactcgttccccttgagataggcacagccaca
cagttggatatagttgcgacattcgaaatcgaccaaaagatgttggaatcaacgctagaggccgatgttctattcaatt
gcacgacaagtgaaggctcggttgcaaggagtgtgttgggaccgtttggtgtggtggttctagccgatgcccagcgctc
cgaacaacttcctgtatacttctatatcgcaaaagatattgatggaacctcacgaacttatttttgtgccgacgaaaca
agatcatccaaggatgtaagcgtagggaaatgggtgtacggaagcagtgttcctgtcctcccaggcgaaaagtacaata
tgaggttattggtggatcattcgatagtagagggatttgcacaaaacgggagaaccgtggtgacatcaagagtgtatcc
aacaaaggcgatctacaacgctgcgaaggtgtttttgttcaacaacgcgactggaatcagtgtgaaggcgtcgatcaag
atctggaagatgggggaagcagaactcaatcctttccctcttcctgggtggactttcgaactttgatactagagaggag
gtgtcacctactagatgcaaacccctgaaccctttacagaccttgaacatgaaccccacacacccctactggaccacca
ccacaacccaccaccacaaaccaccacaaaacctttgttcaccagggttgtgtccggtgtcacctttgttttattcttc
tttggtttcgctatcgtattcattgttctcaaccaacagaactcttctgttcgtatcgtcaccaattcggagaaatctt
ttataaggtattcgcagaccgatcgcttgtcgtgggaacggaccgcttttcattttcagcctgccaagaattttattta
cgatccagatggtcagttgtttcacatgggctggtaccatatgttctatcaatacaacccatacgcaccggtttggggc
aatatgtcatggggtcactcagtgtccaaagacatgatcaactggtacgagctgccagtcgctatggtcccgaccgaat
ggtatgatatcgagggcgtcttatccgggtctaccacggtccttccaaacggtcagatctttgcattgtatactgggaa
cgctaatgatttttcccaattacaatgcaaagctgtacccgtaaacttatctgacccgcttcttattgagtgggtcaag
tatgaggataacccaatcctgtacactccaccagggattgggttaaaggactatcgggacccgtcaacagtctggacag
gtcccgatggaaagcataggatgatcatgggaactaaacgtggcaatacaggcatggtacttgtttactataccactga
ttacacgaactacgagttgttggatgagccgttgcactctgttcccaacaccgatatgtgggaatgcgtcgacttttac
ccggtttcgttaaccaatgatagtgcacttgatatggcggcctatgggtcgggtatcaaacacgttattaaagaaagtt
gggagggacatggaatggattggtattcaatcgggacatatgacgcgataaatgataaatggactcccgataacccgga
actagatgtcggtatcgggttacggtgcgattacgggaggttttttgcatcaaagagtctttatgacccattgaagaaa
aggaggatcacttggggttatgttggagaatcagatagtgctgatcaggacctctcaagaggatgggctactgtttata
atgttggaagaacaattgtactagatagaaagaccgggacccatttacttcattggcccgttgaggaagtcgagagttt
gagatacaacggtcaggagtttaaagagatcaagctagagcccggttcaatcattccactcgacataggcacggctaca
cagttggacatagttgcaacatttgaggtggatcaagcagcgttgaacgcgacaagtgaaaccgatgatatttatggtt
gcaccactagcttaggtgcagcccaaaggggaagtttgggaccatttggtcttgcggttctagccgatggaaccctttc
tgagttaactccggtttatttctatatagctaaaaaggcagatggaggtgtgtcgacacatttttgtaccgataagcta
aggtcatcactagattatgatggggagagagtggtgtatgggggcactgttcctgtgttagatgatgaagaactcacaa
tgaggctattggtggatcattcgatagtggaggggtttgcgcaaggaggaaggacggttataacatcaagggcgtatcc
aacaaaagcgatatacgaacaagcgaagctgttcttgttcaacaacgccacaggtacgagtgtgaaagcatctctcaag
atttggcaaatggcttctgcaccaattcatcaataccctttttaatactagagtcacacaggaaagtactagatgcgta
aaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgt
cagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgtt
ccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatg
actttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagac
acgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatgga
aacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaa
tcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactcc
aattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaa
aagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataat
aatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata