BBa_K899004
type: ComponentDefinition
persistent identity: BBa K899004
display id: BBa_K899004
version: 1
was generated by: iGEM to SBOL conversion
description: Reporter system for SST and FFT (BBa_K899000 and BBa_K899001)
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:42Z
mutable provenance: partsregistry
owned by: james
owned by: myers
mutable description: Reporter system to test for transcription of fructan fructan fructosyltransferase (FFT) and Sucrose sucrose fructosyltransferase (SST) genes with green flourescent protein (GFP) as reporter, and lacl as promoter
discontinued: false
dominant: false
group u list: _1164_
m user id: 0
owner id: 12807
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Andreas Kj??r
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_E0240
component: BBa_K899001
component: BBa_K899009
component: BBa_K899000
component: BBa_K899010
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_K899010
sequence annotation: BBa_K899000
sequence annotation: BBa_K899009
sequence annotation: BBa_K899001
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K899004_sequence/1

BBa_E0240
type: Component
persistent identity: component2202681
display id: component2202681
version: 1
top level: BBa_K899004
definition: GFP report
access: public

BBa_K899001
type: Component
persistent identity: component2202670
display id: component2202670
version: 1
top level: BBa_K899004
definition: BBa_K899001
access: public

BBa_K899009
type: Component
persistent identity: component2202667
display id: component2202667
version: 1
top level: BBa_K899004
definition: BBa_K899009
access: public

BBa_K899000
type: Component
persistent identity: component2202664
display id: component2202664
version: 1
top level: BBa_K899004
definition: BBa_K899000
access: public

BBa_K899010
type: Component
persistent identity: component2202661
display id: component2202661
version: 1
top level: BBa_K899004
definition: BBa_K899010
access: public

BBa_R0010
type: Component
persistent identity: component2202652
display id: component2202652
version: 1
top level: BBa_K899004
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2202652
display id: annotation2202652
version: 1
top level: BBa_K899004
location: https://synbiohub.org/public/igem/BBa_K899004/annotation2202652/range2202652/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K899004/annotation2202652/range2202652/1
type: Range
persistent identity: range2202652
display id: range2202652
version: 1
top level: BBa_K899004
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K899010
type: SequenceAnnotation
persistent identity: annotation2202661
display id: annotation2202661
version: 1
top level: BBa_K899004
location: https://synbiohub.org/public/igem/BBa_K899004/annotation2202661/range2202661/1
component: BBa_K899010

https://synbiohub.org/public/igem/BBa_K899004/annotation2202661/range2202661/1
type: Range
persistent identity: range2202661
display id: range2202661
version: 1
top level: BBa_K899004
direction: direction/reverse
start: 209
end: 221
orientation: inline

BBa_K899000
type: SequenceAnnotation
persistent identity: annotation2202664
display id: annotation2202664
version: 1
top level: BBa_K899004
location: https://synbiohub.org/public/igem/BBa_K899004/annotation2202664/range2202664/1
component: BBa_K899000

https://synbiohub.org/public/igem/BBa_K899004/annotation2202664/range2202664/1
type: Range
persistent identity: range2202664
display id: range2202664
version: 1
top level: BBa_K899004
direction: direction/reverse
start: 228
end: 2120
orientation: inline

BBa_K899009
type: SequenceAnnotation
persistent identity: annotation2202667
display id: annotation2202667
version: 1
top level: BBa_K899004
location: https://synbiohub.org/public/igem/BBa_K899004/annotation2202667/range2202667/1
component: BBa_K899009

https://synbiohub.org/public/igem/BBa_K899004/annotation2202667/range2202667/1
type: Range
persistent identity: range2202667
display id: range2202667
version: 1
top level: BBa_K899004
direction: direction/reverse
start: 2129
end: 2141
orientation: inline

BBa_K899001
type: SequenceAnnotation
persistent identity: annotation2202670
display id: annotation2202670
version: 1
top level: BBa_K899004
location: https://synbiohub.org/public/igem/BBa_K899004/annotation2202670/range2202670/1
component: BBa_K899001

https://synbiohub.org/public/igem/BBa_K899004/annotation2202670/range2202670/1
type: Range
persistent identity: range2202670
display id: range2202670
version: 1
top level: BBa_K899004
direction: direction/reverse
start: 2148
end: 3995
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2202681
display id: annotation2202681
version: 1
top level: BBa_K899004
location: https://synbiohub.org/public/igem/BBa_K899004/annotation2202681/range2202681/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K899004/annotation2202681/range2202681/1
type: Range
persistent identity: range2202681
display id: range2202681
version: 1
top level: BBa_K899004
direction: direction/reverse
start: 4004
end: 4879
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

GFP report
type: ComponentDefinition
persistent identity: BBa E0240
display id: BBa_E0240
version: 1
was generated by: iGEM to SBOL conversion
description: GFP generator
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0032.E0040.B0015
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0032
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0240_sequence/1

BBa_B0032
type: Component
persistent identity: component1249213
display id: component1249213
version: 1
top level: GFP report
definition: BBa_B0032
access: public

BBa_B0010
type: Component
persistent identity: component1249221
display id: component1249221
version: 1
top level: GFP report
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1249216
display id: component1249216
version: 1
top level: GFP report
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1249231
display id: component1249231
version: 1
top level: GFP report
definition: BBa_B0012
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1249213
display id: annotation1249213
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
type: Range
persistent identity: range1249213
display id: range1249213
version: 1
top level: GFP report
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249216
display id: annotation1249216
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
type: Range
persistent identity: range1249216
display id: range1249216
version: 1
top level: GFP report
direction: direction/forward
start: 20
end: 739
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249221
display id: annotation1249221
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
type: Range
persistent identity: range1249221
display id: range1249221
version: 1
top level: GFP report
direction: direction/forward
start: 748
end: 827
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249231
display id: annotation1249231
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
type: Range
persistent identity: range1249231
display id: range1249231
version: 1
top level: GFP report
direction: direction/forward
start: 836
end: 876
orientation: inline

BBa_K899000
type: ComponentDefinition
persistent identity: BBa K899000
display id: BBa_K899000
version: 1
was generated by: iGEM to SBOL conversion
description: Sucrose sucrose fructosyltransferase (SST)
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:42Z
mutable provenance: Extracted from Jerusalem artichoke (Helianthus tuberosus).
owned by: james
owned by: myers
mutable description: Catalyzes sucrose to the three products: D-sucrose, beta-D-fructofuranosyl-(2->1) and alpha-D-glucopyranoside, as such: 2 sucrose -> D-glucose + beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl + alpha-D-glucopyranoside.
discontinued: false
dominant: false
experience: experience/None
group u list: _1164_
m user id: 0
owner id: 12807
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We removed illigal restriction sites; we added a Shinedalgarno sequence 7 bp from the coding part, to insure expression, while keeping the Kozak sequence to ensure that the part can still be expressed in plants.
star: false
creator: Andreas Kj??r
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start codon
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K899000_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2193663
display id: annotation2193663
version: 1
top level: BBa_K899000
location: https://synbiohub.org/public/igem/BBa_K899000/annotation2193663/range2193663/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K899000/annotation2193663/range2193663/1
type: Range
persistent identity: range2193663
display id: range2193663
version: 1
top level: BBa_K899000
direction: direction/forward
start: 1
end: 3
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2193664
display id: annotation2193664
version: 1
top level: BBa_K899000
location: https://synbiohub.org/public/igem/BBa_K899000/annotation2193664/range2193664/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K899000/annotation2193664/range2193664/1
type: Range
persistent identity: range2193664
display id: range2193664
version: 1
top level: BBa_K899000
direction: direction/forward
start: 1891
end: 1893
orientation: inline

BBa_K899001
type: ComponentDefinition
persistent identity: BBa K899001
display id: BBa_K899001
version: 1
was generated by: iGEM to SBOL conversion
description: Fructan fructan fructosyltransferase (FFT)
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:42Z
mutable provenance: Extracted from Jesrusalem Artichoke (helianthus tuberosus)
owned by: james
owned by: myers
mutable description: Elongates a trisaccharide with the fructose unit of a sucrose substrate into an inulin polymer. Works together with part BBa_K899000 (SST).
discontinued: false
dominant: false
experience: experience/None
group u list: _1164_
m user id: 0
owner id: 12807
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: 7 BP from start codon we embedded an shine dalgarno sequence.
star: false
creator: Andreas Kj??r
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K899001_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2193672
display id: annotation2193672
version: 1
top level: BBa_K899001
location: https://synbiohub.org/public/igem/BBa_K899001/annotation2193672/range2193672/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K899001/annotation2193672/range2193672/1
type: Range
persistent identity: range2193672
display id: range2193672
version: 1
top level: BBa_K899001
direction: direction/forward
start: 1
end: 3
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2193673
display id: annotation2193673
version: 1
top level: BBa_K899001
location: https://synbiohub.org/public/igem/BBa_K899001/annotation2193673/range2193673/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K899001/annotation2193673/range2193673/1
type: Range
persistent identity: range2193673
display id: range2193673
version: 1
top level: BBa_K899001
direction: direction/forward
start: 1846
end: 1848
orientation: inline

BBa_K899009
type: ComponentDefinition
persistent identity: BBa K899009
display id: BBa_K899009
version: 1
was generated by: iGEM to SBOL conversion
description: Doubble ribosomal binding site (FFT) (shine-dalgarno and kozak sequence)
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:42Z
mutable provenance: jerusalem artichoke (Helianthus Tuberosus) and modified with bacterial shine-dalgarno sequence
owned by: james
owned by: myers
mutable description: This is the ribosomal binding site(s) we used in front of our fructan:fructan fructosyltransferase (FFT. It consists of the kozak sequence originally from the gene when we extracted it from the jerusalem artichoke (Helianthus Tuberosus), and the shine dalgarno sequence aggaggt.
discontinued: false
dominant: false
experience: experience/None
group u list: _1164_
m user id: 0
owner id: 12807
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: the shine dalgarno is placed 6 bp from the startcodon (optimal distance) and kozak is 4 of theese bp right before start codon.
star: false
creator: Andreas Kj??r
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Shine-Dalgarno sequence
sequence annotation: Kozak sequence
sequence: https://synbiohub.org/public/igem/BBa_K899009_sequence/1

Shine-Dalgarno sequence
type: SequenceAnnotation
persistent identity: annotation2201693
display id: annotation2201693
version: 1
top level: BBa_K899009
location: https://synbiohub.org/public/igem/BBa_K899009/annotation2201693/range2201693/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K899009/annotation2201693/range2201693/1
type: Range
persistent identity: range2201693
display id: range2201693
version: 1
top level: BBa_K899009
direction: direction/forward
start: 1
end: 7
orientation: inline

Kozak sequence
type: SequenceAnnotation
persistent identity: annotation2201692
display id: annotation2201692
version: 1
top level: BBa_K899009
location: https://synbiohub.org/public/igem/BBa_K899009/annotation2201692/range2201692/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K899009/annotation2201692/range2201692/1
type: Range
persistent identity: range2201692
display id: range2201692
version: 1
top level: BBa_K899009
direction: direction/forward
start: 10
end: 13
orientation: inline

BBa_K899010
type: ComponentDefinition
persistent identity: BBa K899010
display id: BBa_K899010
version: 1
was generated by: iGEM to SBOL conversion
description: Doubble ribosomal binding site (SST) (shine-dalgarno and kozak sequence)
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:42Z
mutable provenance: Kozak sequence derives from jerusalem artichoke (helianthus tuberosus) shinedalgarno was introduced by primers.
owned by: james
owned by: myers
mutable description: This sequence is taken from the RBS section of our sucrose:sucrose fructosyltransferase (SST) gene. It consists of a shine dalgarno sequence (AGGAGGT) and a kozak sequence (CACC).
discontinued: false
dominant: false
experience: experience/None
group u list: _1164_
m user id: 0
owner id: 12807
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Andreas Kj??r
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Shine-Dalgarno sequence
sequence annotation: Kozak sequence
sequence: https://synbiohub.org/public/igem/BBa_K899010_sequence/1

Shine-Dalgarno sequence
type: SequenceAnnotation
persistent identity: annotation2201787
display id: annotation2201787
version: 1
top level: BBa_K899010
location: https://synbiohub.org/public/igem/BBa_K899010/annotation2201787/range2201787/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K899010/annotation2201787/range2201787/1
type: Range
persistent identity: range2201787
display id: range2201787
version: 1
top level: BBa_K899010
direction: direction/forward
start: 1
end: 7
orientation: inline

Kozak sequence
type: SequenceAnnotation
persistent identity: annotation2201790
display id: annotation2201790
version: 1
top level: BBa_K899010
location: https://synbiohub.org/public/igem/BBa_K899010/annotation2201790/range2201790/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K899010/annotation2201790/range2201790/1
type: Range
persistent identity: range2201790
display id: range2201790
version: 1
top level: BBa_K899010
direction: direction/forward
start: 10
end: 13
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

https://synbiohub.org/public/igem/BBa_K899004_sequence/1
type: Sequence
persistent identity: BBa K899004 sequence
display id: BBa_K899004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaggaggttacacctactagatgatggcttcatccaccaccaccacccctctcattctccatgatgaccctgaaaacctcccagaactcaccggttctccgacaactcgtcgtctatccatcgcaaaagtgctttcggggatccttgtttcggttctggttataggtgctcttgttgctttaatcaacaaccaaacatatgaatccccctcggccaccacattcgtaactcagttgccaaatattgatctgaagcgggttccaggaaagttggattcgagtgctgaggttgaatggcaacgatccacttatcattttcaacccgacaaaaatttcattagcgatcctgatggcccaatgtatcacatgggatggtatcatctattttatcagtacaaccctcaatctgccatctggggcaacatcacatggggccactcggtatcgaaagacatgatcaactggttccatctccctttcgccatggttcctgaccattggtacgacatcgaaggtgtcatgacgggttcggctacagtcctccctaatggtcaaatcatcatgctttactcgggcaacgcgtatgatctctcccaagtacaatgcttggcgtacgctgtcaactcgtcggatccacttcttatagagtggaaaaaatatgaaggtaaccctgtcttactcccaccaccaggagtaggctacaaggactttcgggacccatccacattgtggtcgggccctgatggtgaatatagaatggtaatggggtccaagcacaacgagactattggctgtgctttgatttaccataccactaattttacgcattttgaattgaaagaggaggtgcttcatgcagtcccacatactggtatgtgggaatgtgttgatctttacccggtgtccaccgtacacacaaacgggctggacatggtggataacgggccaaatgttaagtacgtgttgaaacaaagtggggatgaagatcgccatgattggtatgcaattggaagttacgatatagtgaatgataagtggtacccagatgacccggaaaatgatgtgggtatcggattaagatatgattttggaaaattttatgcgtccaagacgttttatgaccaacataagaagaggagagtcctttggggctatgttggagaaaccgatccccaaaagtatgacctttcaaagggatgggctaacattttgaatattccaaggaccgtcgttttggacctcgaaactaaaaccaatttgattcaatggccaatcgaggaaaccgaaaaccttaggtcgaaaaagtatgatgaatttaaagacgtcgagcttcgacccggggcactcgttccccttgagataggcacagccacacagttggatatagttgcgacattcgaaatcgaccaaaagatgttggaatcaacgctagaggccgatgttctattcaattgcacgacaagtgaaggctcggttgcaaggagtgtgttgggaccgtttggtgtggtggttctagccgatgcccagcgctccgaacaacttcctgtatacttctatatcgcaaaagatattgatggaacctcacgaacttatttttgtgccgacgaaacaagatcatccaaggatgtaagcgtagggaaatgggtgtacggaagcagtgttcctgtcctcccaggcgaaaagtacaatatgaggttattggtggatcattcgatagtagagggatttgcacaaaacgggagaaccgtggtgacatcaagagtgtatccaacaaaggcgatctacaacgctgcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0240_sequence/1
type: Sequence
persistent identity: BBa E0240 sequence
display id: BBa_E0240_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K899000_sequence/1
type: Sequence
persistent identity: BBa K899000 sequence
display id: BBa_K899000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatggcttcatccaccaccaccacccctctcattctccatgatgaccctgaaaacctcccagaactcaccggttctccgacaactcgtcgtctatccatcgcaaaagtgctttcggggatccttgtttcggttctggttataggtgctcttgttgctttaatcaacaaccaaacatatgaatccccctcggccaccacattcgtaactcagttgccaaatattgatctgaagcgggttccaggaaagttggattcgagtgctgaggttgaatggcaacgatccacttatcattttcaacccgacaaaaatttcattagcgatcctgatggcccaatgtatcacatgggatggtatcatctattttatcagtacaaccctcaatctgccatctggggcaacatcacatggggccactcggtatcgaaagacatgatcaactggttccatctccctttcgccatggttcctgaccattggtacgacatcgaaggtgtcatgacgggttcggctacagtcctccctaatggtcaaatcatcatgctttactcgggcaacgcgtatgatctctcccaagtacaatgcttggcgtacgctgtcaactcgtcggatccacttcttatagagtggaaaaaatatgaaggtaaccctgtcttactcccaccaccaggagtaggctacaaggactttcgggacccatccacattgtggtcgggccctgatggtgaatatagaatggtaatggggtccaagcacaacgagactattggctgtgctttgatttaccataccactaattttacgcattttgaattgaaagaggaggtgcttcatgcagtcccacatactggtatgtgggaatgtgttgatctttacccggtgtccaccgtacacacaaacgggctggacatggtggataacgggccaaatgttaagtacgtgttgaaacaaagtggggatgaagatcgccatgattggtatgcaattggaagttacgatatagtgaatgataagtggtacccagatgacccggaaaatgatgtgggtatcggattaagatatgattttggaaaattttatgcgtccaagacgttttatgaccaacataagaagaggagagtcctttggggctatgttggagaaaccgatccccaaaagtatgacctttcaaagggatgggctaacattttgaatattccaaggaccgtcgttttggacctcgaaactaaaaccaatttgattcaatggccaatcgaggaaaccgaaaaccttaggtcgaaaaagtatgatgaatttaaagacgtcgagcttcgacccggggcactcgttccccttgagataggcacagccacacagttggatatagttgcgacattcgaaatcgaccaaaagatgttggaatcaacgctagaggccgatgttctattcaattgcacgacaagtgaaggctcggttgcaaggagtgtgttgggaccgtttggtgtggtggttctagccgatgcccagcgctccgaacaacttcctgtatacttctatatcgcaaaagatattgatggaacctcacgaacttatttttgtgccgacgaaacaagatcatccaaggatgtaagcgtagggaaatgggtgtacggaagcagtgttcctgtcctcccaggcgaaaagtacaatatgaggttattggtggatcattcgatagtagagggatttgcacaaaacgggagaaccgtggtgacatcaagagtgtatccaacaaaggcgatctacaacgctgcgaaggtgtttttgttcaacaacgcgactggaatcagtgtgaaggcgtcgatcaagatctggaagatgggggaagcagaactcaatcctttccctcttcctgggtggactttcgaactttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K899001_sequence/1
type: Sequence
persistent identity: BBa K899001 sequence
display id: BBa_K899001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaaacccctgaaccctttacagaccttgaacatgaaccccacacacccctactggaccaccaccacaacccaccaccacaaaccaccacaaaacctttgttcaccagggttgtgtccggtgtcacctttgttttattcttctttggtttcgctatcgtattcattgttctcaaccaacagaactcttctgttcgtatcgtcaccaattcggagaaatcttttataaggtattcgcagaccgatcgcttgtcgtgggaacggaccgcttttcattttcagcctgccaagaattttatttacgatccagatggtcagttgtttcacatgggctggtaccatatgttctatcaatacaacccatacgcaccggtttggggcaatatgtcatggggtcactcagtgtccaaagacatgatcaactggtacgagctgccagtcgctatggtcccgaccgaatggtatgatatcgagggcgtcttatccgggtctaccacggtccttccaaacggtcagatctttgcattgtatactgggaacgctaatgatttttcccaattacaatgcaaagctgtacccgtaaacttatctgacccgcttcttattgagtgggtcaagtatgaggataacccaatcctgtacactccaccagggattgggttaaaggactatcgggacccgtcaacagtctggacaggtcccgatggaaagcataggatgatcatgggaactaaacgtggcaatacaggcatggtacttgtttactataccactgattacacgaactacgagttgttggatgagccgttgcactctgttcccaacaccgatatgtgggaatgcgtcgacttttacccggtttcgttaaccaatgatagtgcacttgatatggcggcctatgggtcgggtatcaaacacgttattaaagaaagttgggagggacatggaatggattggtattcaatcgggacatatgacgcgataaatgataaatggactcccgataacccggaactagatgtcggtatcgggttacggtgcgattacgggaggttttttgcatcaaagagtctttatgacccattgaagaaaaggaggatcacttggggttatgttggagaatcagatagtgctgatcaggacctctcaagaggatgggctactgtttataatgttggaagaacaattgtactagatagaaagaccgggacccatttacttcattggcccgttgaggaagtcgagagtttgagatacaacggtcaggagtttaaagagatcaagctagagcccggttcaatcattccactcgacataggcacggctacacagttggacatagttgcaacatttgaggtggatcaagcagcgttgaacgcgacaagtgaaaccgatgatatttatggttgcaccactagcttaggtgcagcccaaaggggaagtttgggaccatttggtcttgcggttctagccgatggaaccctttctgagttaactccggtttatttctatatagctaaaaaggcagatggaggtgtgtcgacacatttttgtaccgataagctaaggtcatcactagattatgatggggagagagtggtgtatgggggcactgttcctgtgttagatgatgaagaactcacaatgaggctattggtggatcattcgatagtggaggggtttgcgcaaggaggaaggacggttataacatcaagggcgtatccaacaaaagcgatatacgaacaagcgaagctgttcttgttcaacaacgccacaggtacgagtgtgaaagcatctctcaagatttggcaaatggcttctgcaccaattcatcaataccctttttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K899009_sequence/1
type: Sequence
persistent identity: BBa K899009 sequence
display id: BBa_K899009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggaggtgtcacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K899010_sequence/1
type: Sequence
persistent identity: BBa K899010 sequence
display id: BBa_K899010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggaggttacacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z