LOCUS BBa_K899005 2741 bp DNA linear 23-Dec-2024 DEFINITION heat inducible SST with TA system compatible with BBa_K899006 ACCESSION BBa_K899005 VERSION BBa_K899005.1 FEATURES Location/Qualifiers promoter 1..98 /label=BBa_K338001 misc_feature 105..107 /label=Start codon misc_feature 1995..1997 /label=Stop codon CDS 105..1997 /label=BBa_K899000 misc_feature 2010..2013 /label=conserved RBS 2006..2017 /label=BBa_B0034 CDS 2024..2329 /label=BBa_K899007 misc_feature 2342..2345 /label=conserved RBS 2338..2349 /label=BBa_B0034 CDS 2356..2604 /label=BBa_K302032 stem_loop 2624..2667 /label=stem_loop misc_feature 2613..2692 /label=BBa_B0010 terminator 2613..2692 /label=BBa_B0010 stem_loop 2708..2727 /label=T7 TE misc_feature 2734..2734 /label=stop polyA_site 2728..2741 /label=polya misc_feature 2701..2741 /label=BBa_B0012 terminator 2701..2741 /label=BBa_B0012 terminator 2613..2741 /label=BBa_B0015 ORIGIN 1 ccgaggtcct tgttgcgaag attgatgaca atgtgagtgc ttcccttgaa accctgaaac 61 tgatccccat aataagcgaa gttagcgaga tgaatgcgta ctagatgatg gcttcatcca 121 ccaccaccac ccctctcatt ctccatgatg accctgaaaa cctcccagaa ctcaccggtt 181 ctccgacaac tcgtcgtcta tccatcgcaa aagtgctttc ggggatcctt gtttcggttc 241 tggttatagg tgctcttgtt gctttaatca acaaccaaac atatgaatcc ccctcggcca 301 ccacattcgt aactcagttg ccaaatattg atctgaagcg ggttccagga aagttggatt 361 cgagtgctga ggttgaatgg caacgatcca cttatcattt tcaacccgac aaaaatttca 421 ttagcgatcc tgatggccca atgtatcaca tgggatggta tcatctattt tatcagtaca 481 accctcaatc tgccatctgg ggcaacatca catggggcca ctcggtatcg aaagacatga 541 tcaactggtt ccatctccct ttcgccatgg ttcctgacca ttggtacgac atcgaaggtg 601 tcatgacggg ttcggctaca gtcctcccta atggtcaaat catcatgctt tactcgggca 661 acgcgtatga tctctcccaa gtacaatgct tggcgtacgc tgtcaactcg tcggatccac 721 ttcttataga gtggaaaaaa tatgaaggta accctgtctt actcccacca ccaggagtag 781 gctacaagga ctttcgggac ccatccacat tgtggtcggg ccctgatggt gaatatagaa 841 tggtaatggg gtccaagcac aacgagacta ttggctgtgc tttgatttac cataccacta 901 attttacgca ttttgaattg aaagaggagg tgcttcatgc agtcccacat actggtatgt 961 gggaatgtgt tgatctttac ccggtgtcca ccgtacacac aaacgggctg gacatggtgg 1021 ataacgggcc aaatgttaag tacgtgttga aacaaagtgg ggatgaagat cgccatgatt 1081 ggtatgcaat tggaagttac gatatagtga atgataagtg gtacccagat gacccggaaa 1141 atgatgtggg tatcggatta agatatgatt ttggaaaatt ttatgcgtcc aagacgtttt 1201 atgaccaaca taagaagagg agagtccttt ggggctatgt tggagaaacc gatccccaaa 1261 agtatgacct ttcaaaggga tgggctaaca ttttgaatat tccaaggacc gtcgttttgg 1321 acctcgaaac taaaaccaat ttgattcaat ggccaatcga ggaaaccgaa aaccttaggt 1381 cgaaaaagta tgatgaattt aaagacgtcg agcttcgacc cggggcactc gttccccttg 1441 agataggcac agccacacag ttggatatag ttgcgacatt cgaaatcgac caaaagatgt 1501 tggaatcaac gctagaggcc gatgttctat tcaattgcac gacaagtgaa ggctcggttg 1561 caaggagtgt gttgggaccg tttggtgtgg tggttctagc cgatgcccag cgctccgaac 1621 aacttcctgt atacttctat atcgcaaaag atattgatgg aacctcacga acttattttt 1681 gtgccgacga aacaagatca tccaaggatg taagcgtagg gaaatgggtg tacggaagca 1741 gtgttcctgt cctcccaggc gaaaagtaca atatgaggtt attggtggat cattcgatag 1801 tagagggatt tgcacaaaac gggagaaccg tggtgacatc aagagtgtat ccaacaaagg 1861 cgatctacaa cgctgcgaag gtgtttttgt tcaacaacgc gactggaatc agtgtgaagg 1921 cgtcgatcaa gatctggaag atgggggaag cagaactcaa tcctttccct cttcctgggt 1981 ggactttcga actttgatac tagagaaaga ggagaaatac tagatgcagt ttaaggttta 2041 cacctataaa agagagagcc gttatcgtct gtttgtggat gtccagagtg acattattga 2101 cacgcccggg cgacggatgg tgatccccct ggccagcgcc cgcctactgt cagacaaagt 2161 ctcccgtgag ctttacccgg tggtgcatgt cggggatgaa agctggcgca tgatgaccac 2221 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggccgatc tcagccaccg 2281 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataat actagagaaa 2341 gaggagaaat actagatgat ccacagtagc gtaaagcgtt ggggaaattc accggcggtg 2401 cggatcccgg ctacgttaat gcaggcgctc aatctgaata ttgatgatga agtgaagatt 2461 gacctggtgg atggcaaatt aattattgag ccagtgcgta aagagcccgt atttacgctt 2521 gctgaactgg tcaacgacat cacgccggaa aacctccacg agaatatcga ctggggagag 2581 ccgaaagata aggaagtctg gtaatactag agccaggcat caaataaaac gaaaggctca 2641 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 2701 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //