BBa_K899005
type: ComponentDefinition
persistent identity: BBa K899005
display id: BBa_K899005
version: 1
was generated by: iGEM to SBOL conversion
description: heat inducible SST with TA system compatible with BBa_K899006
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:42Z
mutable provenance: partsregistry
owned by: james
owned by: myers
mutable description: Heat inducible promoter K33801 regulating the expression of sucrose sucrose fructosyltransferase (SST) gene and a toxin-antitoxin (TA) system compatible with part BBa_K899006. The TA system in this part contains ccdB as a toxin and MazE as antitoxin.
discontinued: false
dominant: false
experience: experience/None
group u list: _1164_
m user id: 0
owner id: 12807
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: none
star: false
creator: Andreas Kj??r
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_K302032
component: BBa_B0034
component: BBa_K899007
component: BBa_K899000
component: BBa_K338001
sequence annotation: BBa_K338001
sequence annotation: BBa_K899000
sequence annotation: BBa_B0034
sequence annotation: BBa_K899007
sequence annotation: BBa_K302032
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K899005_sequence/1

BBa_B0015
type: Component
persistent identity: component2226806
display id: component2226806
version: 1
top level: BBa_K899005
definition: BBa_B0015
access: public

BBa_K302032
type: Component
persistent identity: component2226799
display id: component2226799
version: 1
top level: BBa_K899005
definition: BBa_K302032
access: public

BBa_B0034
type: Component
persistent identity: component2226798
display id: component2226798
version: 1
top level: BBa_K899005
definition: BBa_B0034
access: public

BBa_K899007
type: Component
persistent identity: component2226796
display id: component2226796
version: 1
top level: BBa_K899005
definition: BBa_K899007
access: public

BBa_B0034
type: Component
persistent identity: component2226795
display id: component2226795
version: 1
top level: BBa_K899005
definition: BBa_B0034
access: public

BBa_K899000
type: Component
persistent identity: component2226793
display id: component2226793
version: 1
top level: BBa_K899005
definition: BBa_K899000
access: public

BBa_K338001
type: Component
persistent identity: component2226790
display id: component2226790
version: 1
top level: BBa_K899005
definition: HSP
access: public

BBa_K338001
type: SequenceAnnotation
persistent identity: annotation2226790
display id: annotation2226790
version: 1
top level: BBa_K899005
location: https://synbiohub.org/public/igem/BBa_K899005/annotation2226790/range2226790/1
component: BBa_K338001

https://synbiohub.org/public/igem/BBa_K899005/annotation2226790/range2226790/1
type: Range
persistent identity: range2226790
display id: range2226790
version: 1
top level: BBa_K899005
direction: direction/reverse
start: 1
end: 98
orientation: inline

BBa_K899000
type: SequenceAnnotation
persistent identity: annotation2226793
display id: annotation2226793
version: 1
top level: BBa_K899005
location: https://synbiohub.org/public/igem/BBa_K899005/annotation2226793/range2226793/1
component: BBa_K899000

https://synbiohub.org/public/igem/BBa_K899005/annotation2226793/range2226793/1
type: Range
persistent identity: range2226793
display id: range2226793
version: 1
top level: BBa_K899005
direction: direction/reverse
start: 105
end: 1997
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2226795
display id: annotation2226795
version: 1
top level: BBa_K899005
location: https://synbiohub.org/public/igem/BBa_K899005/annotation2226795/range2226795/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K899005/annotation2226795/range2226795/1
type: Range
persistent identity: range2226795
display id: range2226795
version: 1
top level: BBa_K899005
direction: direction/reverse
start: 2006
end: 2017
orientation: inline

BBa_K899007
type: SequenceAnnotation
persistent identity: annotation2226796
display id: annotation2226796
version: 1
top level: BBa_K899005
location: https://synbiohub.org/public/igem/BBa_K899005/annotation2226796/range2226796/1
component: BBa_K899007

https://synbiohub.org/public/igem/BBa_K899005/annotation2226796/range2226796/1
type: Range
persistent identity: range2226796
display id: range2226796
version: 1
top level: BBa_K899005
direction: direction/reverse
start: 2024
end: 2329
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2226798
display id: annotation2226798
version: 1
top level: BBa_K899005
location: https://synbiohub.org/public/igem/BBa_K899005/annotation2226798/range2226798/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K899005/annotation2226798/range2226798/1
type: Range
persistent identity: range2226798
display id: range2226798
version: 1
top level: BBa_K899005
direction: direction/reverse
start: 2338
end: 2349
orientation: inline

BBa_K302032
type: SequenceAnnotation
persistent identity: annotation2226799
display id: annotation2226799
version: 1
top level: BBa_K899005
location: https://synbiohub.org/public/igem/BBa_K899005/annotation2226799/range2226799/1
component: BBa_K302032

https://synbiohub.org/public/igem/BBa_K899005/annotation2226799/range2226799/1
type: Range
persistent identity: range2226799
display id: range2226799
version: 1
top level: BBa_K899005
direction: direction/reverse
start: 2356
end: 2604
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2226806
display id: annotation2226806
version: 1
top level: BBa_K899005
location: https://synbiohub.org/public/igem/BBa_K899005/annotation2226806/range2226806/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K899005/annotation2226806/range2226806/1
type: Range
persistent identity: range2226806
display id: range2226806
version: 1
top level: BBa_K899005
direction: direction/reverse
start: 2613
end: 2741
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

HSP
type: ComponentDefinition
persistent identity: BBa K338001
display id: BBa_K338001
version: 1
was generated by: iGEM to SBOL conversion
description: Heat Shock Promoter (HSP)
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:07Z
mutable provenance: Modified BBa_K112400
owned by: james
owned by: myers
mutable description: This is a modified K112400 heat shock promoter in BBa standard.
discontinued: false
dominant: false
experience: experience/Works
group u list: _454_
m user id: 0
owner id: 6189
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: None
star: false
creator: 2010 Caltech iGEM Team
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K338001_sequence/1

BBa_K302032
type: ComponentDefinition
persistent identity: BBa K302032
display id: BBa_K302032
version: 1
was generated by: iGEM to SBOL conversion
description: mazE
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: ''Bacillus Subtilis'' 168 strain
owned by: james
owned by: myers
mutable description: Encodes the liable antitoxin to ''mazF'' in ''Bacillus Subtilis''. It is used in conjungtion with mazF in ''Bacillus Subtilis'' to provide a toxin-antitoxin in various stressful conditions. When transcribtion of both genes is turned off (as both are under contol of same promoter) ''mazE'' will be degraded faster than ''mazF''. There is then no inhibiton of ''mazF'', killing the cell.
discontinued: false
dominant: false
experience: experience/None
group u list: _442_
m user id: 0
owner id: 6345
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Sequence isolated based upon the work of Pellegrini, O., Mathy, N., Gogos, A., Shapiro, L., and Condon, C. (2005) The ''Bacillus subtilis'' ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor. ''Mol Microbiol'' 56: 1139???1148.
star: false
creator: Philip Hall
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K302032_sequence/1

BBa_K899000
type: ComponentDefinition
persistent identity: BBa K899000
display id: BBa_K899000
version: 1
was generated by: iGEM to SBOL conversion
description: Sucrose sucrose fructosyltransferase (SST)
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:42Z
mutable provenance: Extracted from Jerusalem artichoke (Helianthus tuberosus).
owned by: james
owned by: myers
mutable description: Catalyzes sucrose to the three products: D-sucrose, beta-D-fructofuranosyl-(2->1) and alpha-D-glucopyranoside, as such: 2 sucrose -> D-glucose + beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl + alpha-D-glucopyranoside.
discontinued: false
dominant: false
experience: experience/None
group u list: _1164_
m user id: 0
owner id: 12807
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We removed illigal restriction sites; we added a Shinedalgarno sequence 7 bp from the coding part, to insure expression, while keeping the Kozak sequence to ensure that the part can still be expressed in plants.
star: false
creator: Andreas Kj??r
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start codon
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K899000_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2193663
display id: annotation2193663
version: 1
top level: BBa_K899000
location: https://synbiohub.org/public/igem/BBa_K899000/annotation2193663/range2193663/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K899000/annotation2193663/range2193663/1
type: Range
persistent identity: range2193663
display id: range2193663
version: 1
top level: BBa_K899000
direction: direction/forward
start: 1
end: 3
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2193664
display id: annotation2193664
version: 1
top level: BBa_K899000
location: https://synbiohub.org/public/igem/BBa_K899000/annotation2193664/range2193664/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K899000/annotation2193664/range2193664/1
type: Range
persistent identity: range2193664
display id: range2193664
version: 1
top level: BBa_K899000
direction: direction/forward
start: 1891
end: 1893
orientation: inline

BBa_K899007
type: ComponentDefinition
persistent identity: BBa K899007
display id: BBa_K899007
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB toxin
created: 2012-09-21T11:00:00Z
modified: 2015-07-09T11:39:55Z
mutable provenance: found in various bacteria including E. coli strains. Sequence found on NCBI from E. coli
owned by: james
owned by: myers
mutable description: ccdB is a toxin with antitoxin ccdA
discontinued: true
dominant: false
experience: experience/None
group u list: _1164_
m user id: 4206
owner id: 12807
owning group id: 9
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable notes: none
star: false
creator: Andreas Kj??r
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K899007_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K899005_sequence/1
type: Sequence
persistent identity: BBa K899005 sequence
display id: BBa_K899005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccgaggtccttgttgcgaagattgatgacaatgtgagtgcttcccttgaaaccctgaaactgatccccataataagcgaagttagcgagatgaatgcgtactagatgatggcttcatccaccaccaccacccctctcattctccatgatgaccctgaaaacctcccagaactcaccggttctccgacaactcgtcgtctatccatcgcaaaagtgctttcggggatccttgtttcggttctggttataggtgctcttgttgctttaatcaacaaccaaacatatgaatccccctcggccaccacattcgtaactcagttgccaaatattgatctgaagcgggttccaggaaagttggattcgagtgctgaggttgaatggcaacgatccacttatcattttcaacccgacaaaaatttcattagcgatcctgatggcccaatgtatcacatgggatggtatcatctattttatcagtacaaccctcaatctgccatctggggcaacatcacatggggccactcggtatcgaaagacatgatcaactggttccatctccctttcgccatggttcctgaccattggtacgacatcgaaggtgtcatgacgggttcggctacagtcctccctaatggtcaaatcatcatgctttactcgggcaacgcgtatgatctctcccaagtacaatgcttggcgtacgctgtcaactcgtcggatccacttcttatagagtggaaaaaatatgaaggtaaccctgtcttactcccaccaccaggagtaggctacaaggactttcgggacccatccacattgtggtcgggccctgatggtgaatatagaatggtaatggggtccaagcacaacgagactattggctgtgctttgatttaccataccactaattttacgcattttgaattgaaagaggaggtgcttcatgcagtcccacatactggtatgtgggaatgtgttgatctttacccggtgtccaccgtacacacaaacgggctggacatggtggataacgggccaaatgttaagtacgtgttgaaacaaagtggggatgaagatcgccatgattggtatgcaattggaagttacgatatagtgaatgataagtggtacccagatgacccggaaaatgatgtgggtatcggattaagatatgattttggaaaattttatgcgtccaagacgttttatgaccaacataagaagaggagagtcctttggggctatgttggagaaaccgatccccaaaagtatgacctttcaaagggatgggctaacattttgaatattccaaggaccgtcgttttggacctcgaaactaaaaccaatttgattcaatggccaatcgaggaaaccgaaaaccttaggtcgaaaaagtatgatgaatttaaagacgtcgagcttcgacccggggcactcgttccccttgagataggcacagccacacagttggatatagttgcgacattcgaaatcgaccaaaagatgttggaatcaacgctagaggccgatgttctattcaattgcacgacaagtgaaggctcggttgcaaggagtgtgttgggaccgtttggtgtggtggttctagccgatgcccagcgctccgaacaacttcctgtatacttctatatcgcaaaagatattgatggaacctcacgaacttatttttgtgccgacgaaacaagatcatccaaggatgtaagcgtagggaaatgggtgtacggaagcagtgttcctgtcctcccaggcgaaaagtacaatatgaggttattggtggatcattcgatagtagagggatttgcacaaaacgggagaaccgtggtgacatcaagagtgtatccaacaaaggcgatctacaacgctgcgaaggtgtttttgttcaacaacgcgactggaatcagtgtgaaggcgtcgatcaagatctggaagatgggggaagcagaactcaatcctttccctcttcctgggtggactttcgaactttgatac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K338001_sequence/1
type: Sequence
persistent identity: BBa K338001 sequence
display id: BBa_K338001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccgaggtccttgttgcgaagattgatgacaatgtgagtgcttcccttgaaaccctgaaactgatccccataataagcgaagttagcgagatgaatgcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K302032_sequence/1
type: Sequence
persistent identity: BBa K302032 sequence
display id: BBa_K302032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatccacagtagcgtaaagcgttggggaaattcaccggcggtgcggatcccggctacgttaatgcaggcgctcaatctgaatattgatgatgaagtgaagattgacctggtggatggcaaattaattattgagccagtgcgtaaagagcccgtatttacgcttgctgaactggtcaacgacatcacgccggaaaacctccacgagaatatcgactggggagagccgaaagataaggaagtctggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K899000_sequence/1
type: Sequence
persistent identity: BBa K899000 sequence
display id: BBa_K899000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatggcttcatccaccaccaccacccctctcattctccatgatgaccctgaaaacctcccagaactcaccggttctccgacaactcgtcgtctatccatcgcaaaagtgctttcggggatccttgtttcggttctggttataggtgctcttgttgctttaatcaacaaccaaacatatgaatccccctcggccaccacattcgtaactcagttgccaaatattgatctgaagcgggttccaggaaagttggattcgagtgctgaggttgaatggcaacgatccacttatcattttcaacccgacaaaaatttcattagcgatcctgatggcccaatgtatcacatgggatggtatcatctattttatcagtacaaccctcaatctgccatctggggcaacatcacatggggccactcggtatcgaaagacatgatcaactggttccatctccctttcgccatggttcctgaccattggtacgacatcgaaggtgtcatgacgggttcggctacagtcctccctaatggtcaaatcatcatgctttactcgggcaacgcgtatgatctctcccaagtacaatgcttggcgtacgctgtcaactcgtcggatccacttcttatagagtggaaaaaatatgaaggtaaccctgtcttactcccaccaccaggagtaggctacaaggactttcgggacccatccacattgtggtcgggccctgatggtgaatatagaatggtaatggggtccaagcacaacgagactattggctgtgctttgatttaccataccactaattttacgcattttgaattgaaagaggaggtgcttcatgcagtcccacatactggtatgtgggaatgtgttgatctttacccggtgtccaccgtacacacaaacgggctggacatggtggataacgggccaaatgttaagtacgtgttgaaacaaagtggggatgaagatcgccatgattggtatgcaattggaagttacgatatagtgaatgataagtggtacccagatgacccggaaaatgatgtgggtatcggattaagatatgattttggaaaattttatgcgtccaagacgttttatgaccaacataagaagaggagagtcctttggggctatgttggagaaaccgatccccaaaagtatgacctttcaaagggatgggctaacattttgaatattccaaggaccgtcgttttggacctcgaaactaaaaccaatttgattcaatggccaatcgaggaaaccgaaaaccttaggtcgaaaaagtatgatgaatttaaagacgtcgagcttcgacccggggcactcgttccccttgagataggcacagccacacagttggatatagttgcgacattcgaaatcgaccaaaagatgttggaatcaacgctagaggccgatgttctattcaattgcacgacaagtgaaggctcggttgcaaggagtgtgttgggaccgtttggtgtggtggttctagccgatgcccagcgctccgaacaacttcctgtatacttctatatcgcaaaagatattgatggaacctcacgaacttatttttgtgccgacgaaacaagatcatccaaggatgtaagcgtagggaaatgggtgtacggaagcagtgttcctgtcctcccaggcgaaaagtacaatatgaggttattggtggatcattcgatagtagagggatttgcacaaaacgggagaaccgtggtgacatcaagagtgtatccaacaaaggcgatctacaacgctgcgaaggtgtttttgttcaacaacgcgactggaatcagtgtgaaggcgtcgatcaagatctggaagatgggggaagcagaactcaatcctttccctcttcctgggtggactttcgaactttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K899007_sequence/1
type: Sequence
persistent identity: BBa K899007 sequence
display id: BBa_K899007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtccagagtgacattattgacacgcccgggcgacggatggtgatccccctggccagcgcccgcctactgtcagacaaagtctcccgtgagctttacccggtggtgcatgtcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggccgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z