LOCUS       BBa_K899006             2720 bp    DNA     linear       26-Dec-2024
DEFINITION  heat inducible FFT with TA system compatible with BBa_K899005
ACCESSION   BBa_K899006
VERSION     BBa_K899006.1  
FEATURES             Location/Qualifiers
     promoter        1..98
                     /label=BBa_K338001
     misc_feature    116..119
                     /label=Kozak sequence
     misc_feature    107..113
                     /label=Shine-Dalgarno sequence
     RBS             107..119
                     /label=BBa_K899009
     misc_feature    126..128
                     /label=Start codon
     misc_feature    1971..1973
                     /label=Stop codon
     CDS             126..1973
                     /label=BBa_K899001
     misc_feature    1986..1989
                     /label=conserved
     RBS             1982..1993
                     /label=BBa_B0034
     misc_feature    2333..2338
                     /label=double TAA added
     CDS             2000..2338
                     /label=BBa_K302033
     misc_feature    2351..2354
                     /label=conserved
     RBS             2347..2358
                     /label=BBa_B0034
     CDS             2365..2583
                     /label=BBa_K899008
     stem_loop       2603..2646
                     /label=stem_loop
     misc_feature    2592..2671
                     /label=BBa_B0010
     terminator      2592..2671
                     /label=BBa_B0010
     stem_loop       2687..2706
                     /label=T7 TE
     misc_feature    2713..2713
                     /label=stop
     polyA_site      2707..2720
                     /label=polya
     misc_feature    2680..2720
                     /label=BBa_B0012
     terminator      2680..2720
                     /label=BBa_B0012
     terminator      2592..2720
                     /label=BBa_B0015
ORIGIN
        1 ccgaggtcct tgttgcgaag attgatgaca atgtgagtgc ttcccttgaa accctgaaac
       61 tgatccccat aataagcgaa gttagcgaga tgaatgcgta ctagagagga ggtgtcacct
      121 actagatgca aacccctgaa ccctttacag accttgaaca tgaaccccac acacccctac
      181 tggaccacca ccacaaccca ccaccacaaa ccaccacaaa acctttgttc accagggttg
      241 tgtccggtgt cacctttgtt ttattcttct ttggtttcgc tatcgtattc attgttctca
      301 accaacagaa ctcttctgtt cgtatcgtca ccaattcgga gaaatctttt ataaggtatt
      361 cgcagaccga tcgcttgtcg tgggaacgga ccgcttttca ttttcagcct gccaagaatt
      421 ttatttacga tccagatggt cagttgtttc acatgggctg gtaccatatg ttctatcaat
      481 acaacccata cgcaccggtt tggggcaata tgtcatgggg tcactcagtg tccaaagaca
      541 tgatcaactg gtacgagctg ccagtcgcta tggtcccgac cgaatggtat gatatcgagg
      601 gcgtcttatc cgggtctacc acggtccttc caaacggtca gatctttgca ttgtatactg
      661 ggaacgctaa tgatttttcc caattacaat gcaaagctgt acccgtaaac ttatctgacc
      721 cgcttcttat tgagtgggtc aagtatgagg ataacccaat cctgtacact ccaccaggga
      781 ttgggttaaa ggactatcgg gacccgtcaa cagtctggac aggtcccgat ggaaagcata
      841 ggatgatcat gggaactaaa cgtggcaata caggcatggt acttgtttac tataccactg
      901 attacacgaa ctacgagttg ttggatgagc cgttgcactc tgttcccaac accgatatgt
      961 gggaatgcgt cgacttttac ccggtttcgt taaccaatga tagtgcactt gatatggcgg
     1021 cctatgggtc gggtatcaaa cacgttatta aagaaagttg ggagggacat ggaatggatt
     1081 ggtattcaat cgggacatat gacgcgataa atgataaatg gactcccgat aacccggaac
     1141 tagatgtcgg tatcgggtta cggtgcgatt acgggaggtt ttttgcatca aagagtcttt
     1201 atgacccatt gaagaaaagg aggatcactt ggggttatgt tggagaatca gatagtgctg
     1261 atcaggacct ctcaagagga tgggctactg tttataatgt tggaagaaca attgtactag
     1321 atagaaagac cgggacccat ttacttcatt ggcccgttga ggaagtcgag agtttgagat
     1381 acaacggtca ggagtttaaa gagatcaagc tagagcccgg ttcaatcatt ccactcgaca
     1441 taggcacggc tacacagttg gacatagttg caacatttga ggtggatcaa gcagcgttga
     1501 acgcgacaag tgaaaccgat gatatttatg gttgcaccac tagcttaggt gcagcccaaa
     1561 ggggaagttt gggaccattt ggtcttgcgg ttctagccga tggaaccctt tctgagttaa
     1621 ctccggttta tttctatata gctaaaaagg cagatggagg tgtgtcgaca catttttgta
     1681 ccgataagct aaggtcatca ctagattatg atggggagag agtggtgtat gggggcactg
     1741 ttcctgtgtt agatgatgaa gaactcacaa tgaggctatt ggtggatcat tcgatagtgg
     1801 aggggtttgc gcaaggagga aggacggtta taacatcaag ggcgtatcca acaaaagcga
     1861 tatacgaaca agcgaagctg ttcttgttca acaacgccac aggtacgagt gtgaaagcat
     1921 ctctcaagat ttggcaaatg gcttctgcac caattcatca ataccctttt taatactaga
     1981 gaaagaggag aaatactaga tggtaagccg atacgtaccc gatatgggcg atctgatttg
     2041 ggttgatttt gacccgacaa aaggtagcga gcaagctgga catcgtccag ctgttgtcct
     2101 gagtcctttc atgtacaaca acaaaacagg tatgtgtctg tgtgttcctt gtacaacgca
     2161 atcaaaagga tatccgttcg aagttgtttt atccggtcag gaacgtgatg gcgtagcgtt
     2221 agctgatcag gtaaaaagta tcgcctggcg ggcaagagga gcaacgaaga aaggaacagt
     2281 tgccccagag gaattacaac tcattaaagc caaaattaac gtactgattg ggtaataata
     2341 ctagagaaag aggagaaata ctagatgaag cagcgcatta cagtcaccgt cgacagtgac
     2401 agctatcagt tgctcaaggc atatgatgtc aatatctccg gtctggtaag caccaccatg
     2461 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cagaaaatca ggaagggatg
     2521 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ccgatgagaa cagggactgg
     2581 tgatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2641 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     2701 tgggcctttc tgcgtttata
//