BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K338001 1 HSP Heat Shock Promoter (HSP) 2010-10-23T11:00:00Z 2015-05-08T01:12:07Z Modified BBa_K112400 This is a modified K112400 heat shock promoter in BBa standard. false false _454_ 0 6189 9 It's complicated true None false 2010 Caltech iGEM Team BBa_K899009 1 BBa_K899009 Doubble ribosomal binding site (FFT) (shine-dalgarno and kozak sequence) 2012-09-25T11:00:00Z 2015-05-08T01:13:42Z jerusalem artichoke (Helianthus Tuberosus) and modified with bacterial shine-dalgarno sequence This is the ribosomal binding site(s) we used in front of our fructan:fructan fructosyltransferase (FFT. It consists of the kozak sequence originally from the gene when we extracted it from the jerusalem artichoke (Helianthus Tuberosus), and the shine dalgarno sequence aggaggt. false false _1164_ 0 12807 9 Not in stock false the shine dalgarno is placed 6 bp from the startcodon (optimal distance) and kozak is 4 of theese bp right before start codon. false Andreas Kj??r annotation2201693 1 Shine-Dalgarno sequence range2201693 1 1 7 annotation2201692 1 Kozak sequence range2201692 1 10 13 BBa_K899008 1 BBa_K899008 antitoxin ccdA 2012-09-21T11:00:00Z 2015-05-08T01:13:42Z This antitoxin is found in E. coli and the sequence is derived from NCBI antitoxin ccdA working by inhibitting the toxin ccdB (BBa_K899007) false false _1164_ 0 12807 9 Not in stock false none false Andreas Kj??r BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K302033 1 BBa_K302033 mazF 2010-10-24T11:00:00Z 2015-06-01T02:27:54Z ''Bacillus Subtilis'' 168 strain Encodes a stable non-specific ribonuclease in ''Bacillus Subtilis''. It is used in conjungtion with ''mazE'' in ''Bacillus Subtilis'' to provide a toxin-antitoxin in various stressful conditions. When transcribtion of both genes is turned off (as both are under contol of same promoter) ''mazE'' will be degraded faster than ''mazF''. There is then no inhibiton of ''mazF'', killing the cell. false false _442_ 4206 6345 9 In stock false Sequence isolated based upon the work of Pellegrini, O., Mathy, N., Gogos, A., Shapiro, L., and Condon, C. (2005) The ''Bacillus subtilis'' ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor. ''Mol Microbiol'' 56: 1139???1148. false Philip Hall annotation2099798 1 double TAA added range2099798 1 334 339 BBa_K899006 1 BBa_K899006 heat inducible FFT with TA system compatible with BBa_K899005 2012-09-21T11:00:00Z 2015-05-08T01:13:42Z mixed Heat induced fructan fructan fructosyltransferase (FFT) gene with toxin mazF and antitoxin ccdA (compatible with the TA system of BBa_K899005) false false _1164_ 0 12807 9 Not in stock true none false Andreas Kj??r component2202820 1 BBa_K899008 component2202813 1 BBa_K899001 component2202807 1 BBa_K338001 component2202815 1 BBa_B0034 component2202810 1 BBa_K899009 component2202827 1 BBa_B0015 component2202817 1 BBa_K302033 component2202819 1 BBa_B0034 annotation2202810 1 BBa_K899009 range2202810 1 107 119 annotation2202827 1 BBa_B0015 range2202827 1 2592 2720 annotation2202817 1 BBa_K302033 range2202817 1 2000 2338 annotation2202819 1 BBa_B0034 range2202819 1 2347 2358 annotation2202815 1 BBa_B0034 range2202815 1 1982 1993 annotation2202807 1 BBa_K338001 range2202807 1 1 98 annotation2202813 1 BBa_K899001 range2202813 1 126 1973 annotation2202820 1 BBa_K899008 range2202820 1 2365 2583 BBa_K899001 1 BBa_K899001 Fructan fructan fructosyltransferase (FFT) 2012-09-21T11:00:00Z 2015-05-08T01:13:42Z Extracted from Jesrusalem Artichoke (helianthus tuberosus) Elongates a trisaccharide with the fructose unit of a sucrose substrate into an inulin polymer. Works together with part BBa_K899000 (SST). false false _1164_ 0 12807 9 It's complicated false 7 BP from start codon we embedded an shine dalgarno sequence. false Andreas Kj??r annotation2193673 1 Stop codon range2193673 1 1846 1848 annotation2193672 1 Start codon range2193672 1 1 3 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K899006_sequence 1 ccgaggtccttgttgcgaagattgatgacaatgtgagtgcttcccttgaaaccctgaaactgatccccataataagcgaagttagcgagatgaatgcgtactagagaggaggtgtcacctactagatgcaaacccctgaaccctttacagaccttgaacatgaaccccacacacccctactggaccaccaccacaacccaccaccacaaaccaccacaaaacctttgttcaccagggttgtgtccggtgtcacctttgttttattcttctttggtttcgctatcgtattcattgttctcaaccaacagaactcttctgttcgtatcgtcaccaattcggagaaatcttttataaggtattcgcagaccgatcgcttgtcgtgggaacggaccgcttttcattttcagcctgccaagaattttatttacgatccagatggtcagttgtttcacatgggctggtaccatatgttctatcaatacaacccatacgcaccggtttggggcaatatgtcatggggtcactcagtgtccaaagacatgatcaactggtacgagctgccagtcgctatggtcccgaccgaatggtatgatatcgagggcgtcttatccgggtctaccacggtccttccaaacggtcagatctttgcattgtatactgggaacgctaatgatttttcccaattacaatgcaaagctgtacccgtaaacttatctgacccgcttcttattgagtgggtcaagtatgaggataacccaatcctgtacactccaccagggattgggttaaaggactatcgggacccgtcaacagtctggacaggtcccgatggaaagcataggatgatcatgggaactaaacgtggcaatacaggcatggtacttgtttactataccactgattacacgaactacgagttgttggatgagccgttgcactctgttcccaacaccgatatgtgggaatgcgtcgacttttacccggtttcgttaaccaatgatagtgcacttgatatggcggcctatgggtcgggtatcaaacacgttattaaagaaagttgggagggacatggaatggattggtattcaatcgggacatatgacgcgataaatgataaatggactcccgataacccggaactagatgtcggtatcgggttacggtgcgattacgggaggttttttgcatcaaagagtctttatgacccattgaagaaaaggaggatcacttggggttatgttggagaatcagatagtgctgatcaggacctctcaagaggatgggctactgtttataatgttggaagaacaattgtactagatagaaagaccgggacccatttacttcattggcccgttgaggaagtcgagagtttgagatacaacggtcaggagtttaaagagatcaagctagagcccggttcaatcattccactcgacataggcacggctacacagttggacatagttgcaacatttgaggtggatcaagcagcgttgaacgcgacaagtgaaaccgatgatatttatggttgcaccactagcttaggtgcagcccaaaggggaagtttgggaccatttggtcttgcggttctagccgatggaaccctttctgagttaactccggtttatttctatatagctaaaaaggcagatggaggtgtgtcgacacatttttgtaccgataagctaaggtcatcactagattatgatggggagagagtggtgtatgggggcactgttcctgtgttagatgatgaagaactcacaatgaggctattggtggatcattcgatagtggaggggtttgcgcaaggaggaaggacggttataacatcaagggcgtatccaacaaaagcgatatacgaacaagcgaagctgttcttgttcaacaacgccacaggtacgagtgtgaaagcatctctcaagatttggcaaatggcttctgcaccaattcatcaataccctttttaatactagagaaagaggagaaatactagatggtaagccgatacgtacccgatatgggcgatctgatttgggttgattttgacccgacaaaaggtagcgagcaagctggacatcgtccagctgttgtcctgagtcctttcatgtacaacaacaaaacaggtatgtgtctgtgtgttccttgtacaacgcaatcaaaaggatatccgttcgaagttgttttatccggtcaggaacgtgatggcgtagcgttagctgatcaggtaaaaagtatcgcctggcgggcaagaggagcaacgaagaaaggaacagttgccccagaggaattacaactcattaaagccaaaattaacgtactgattgggtaataatactagagaaagaggagaaatactagatgaagcagcgcattacagtcaccgtcgacagtgacagctatcagttgctcaaggcatatgatgtcaatatctccggtctggtaagcaccaccatgcagaatgaagcccgtcgtctgcgtgccgaacgctggaaagcagaaaatcaggaagggatggctgaggtcgcccggtttattgaaatgaacggctcttttgccgatgagaacagggactggtgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K899001_sequence 1 atgcaaacccctgaaccctttacagaccttgaacatgaaccccacacacccctactggaccaccaccacaacccaccaccacaaaccaccacaaaacctttgttcaccagggttgtgtccggtgtcacctttgttttattcttctttggtttcgctatcgtattcattgttctcaaccaacagaactcttctgttcgtatcgtcaccaattcggagaaatcttttataaggtattcgcagaccgatcgcttgtcgtgggaacggaccgcttttcattttcagcctgccaagaattttatttacgatccagatggtcagttgtttcacatgggctggtaccatatgttctatcaatacaacccatacgcaccggtttggggcaatatgtcatggggtcactcagtgtccaaagacatgatcaactggtacgagctgccagtcgctatggtcccgaccgaatggtatgatatcgagggcgtcttatccgggtctaccacggtccttccaaacggtcagatctttgcattgtatactgggaacgctaatgatttttcccaattacaatgcaaagctgtacccgtaaacttatctgacccgcttcttattgagtgggtcaagtatgaggataacccaatcctgtacactccaccagggattgggttaaaggactatcgggacccgtcaacagtctggacaggtcccgatggaaagcataggatgatcatgggaactaaacgtggcaatacaggcatggtacttgtttactataccactgattacacgaactacgagttgttggatgagccgttgcactctgttcccaacaccgatatgtgggaatgcgtcgacttttacccggtttcgttaaccaatgatagtgcacttgatatggcggcctatgggtcgggtatcaaacacgttattaaagaaagttgggagggacatggaatggattggtattcaatcgggacatatgacgcgataaatgataaatggactcccgataacccggaactagatgtcggtatcgggttacggtgcgattacgggaggttttttgcatcaaagagtctttatgacccattgaagaaaaggaggatcacttggggttatgttggagaatcagatagtgctgatcaggacctctcaagaggatgggctactgtttataatgttggaagaacaattgtactagatagaaagaccgggacccatttacttcattggcccgttgaggaagtcgagagtttgagatacaacggtcaggagtttaaagagatcaagctagagcccggttcaatcattccactcgacataggcacggctacacagttggacatagttgcaacatttgaggtggatcaagcagcgttgaacgcgacaagtgaaaccgatgatatttatggttgcaccactagcttaggtgcagcccaaaggggaagtttgggaccatttggtcttgcggttctagccgatggaaccctttctgagttaactccggtttatttctatatagctaaaaaggcagatggaggtgtgtcgacacatttttgtaccgataagctaaggtcatcactagattatgatggggagagagtggtgtatgggggcactgttcctgtgttagatgatgaagaactcacaatgaggctattggtggatcattcgatagtggaggggtttgcgcaaggaggaaggacggttataacatcaagggcgtatccaacaaaagcgatatacgaacaagcgaagctgttcttgttcaacaacgccacaggtacgagtgtgaaagcatctctcaagatttggcaaatggcttctgcaccaattcatcaataccctttttaa BBa_K899009_sequence 1 aggaggtgtcacc BBa_K899008_sequence 1 atgaagcagcgcattacagtcaccgtcgacagtgacagctatcagttgctcaaggcatatgatgtcaatatctccggtctggtaagcaccaccatgcagaatgaagcccgtcgtctgcgtgccgaacgctggaaagcagaaaatcaggaagggatggctgaggtcgcccggtttattgaaatgaacggctcttttgccgatgagaacagggactggtga BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K338001_sequence 1 ccgaggtccttgttgcgaagattgatgacaatgtgagtgcttcccttgaaaccctgaaactgatccccataataagcgaagttagcgagatgaatgcg BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K302033_sequence 1 atggtaagccgatacgtacccgatatgggcgatctgatttgggttgattttgacccgacaaaaggtagcgagcaagctggacatcgtccagctgttgtcctgagtcctttcatgtacaacaacaaaacaggtatgtgtctgtgtgttccttgtacaacgcaatcaaaaggatatccgttcgaagttgttttatccggtcaggaacgtgatggcgtagcgttagctgatcaggtaaaaagtatcgcctggcgggcaagaggagcaacgaagaaaggaacagttgccccagaggaattacaactcattaaagccaaaattaacgtactgattgggtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z