heat inducible FFT with TA system compatible with BBa_K899005
Heat induced fructan fructan fructosyltransferase (FFT) gene with toxin mazF and antitoxin ccdA (compatible with the TA system of BBa_K899005)
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Double terminator consisting of BBa_B0010 and BBa_B0012
This is a modified K112400 heat shock promoter in BBa standard.
Encodes a stable non-specific ribonuclease in ''Bacillus Subtilis''. It is used in conjungtion with ''mazE'' in ''Bacillus Subtilis'' to provide a toxin-antitoxin in various stressful conditions. When transcribtion of both genes is turned off (as both are under contol of same promoter) ''mazE'' will be degraded faster than ''mazF''. There is then no inhibiton of ''mazF'', killing the cell.
Sequence isolated based upon the work of Pellegrini, O., Mathy, N., Gogos, A., Shapiro, L., and Condon, C. (2005) The ''Bacillus subtilis'' ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor. ''Mol Microbiol'' 56: 1139???1148.
Fructan fructan fructosyltransferase (FFT)
Extracted from Jesrusalem Artichoke (helianthus tuberosus)
Elongates a trisaccharide with the fructose unit of a sucrose substrate into an inulin polymer. Works together with part BBa_K899000 (SST).
7 BP from start codon we embedded an shine dalgarno sequence.
Doubble ribosomal binding site (FFT) (shine-dalgarno and kozak sequence)
jerusalem artichoke (Helianthus Tuberosus) and modified with bacterial shine-dalgarno sequence
This is the ribosomal binding site(s) we used in front of our fructan:fructan fructosyltransferase (FFT. It consists of the kozak sequence originally from the gene when we extracted it from the jerusalem artichoke (Helianthus Tuberosus), and the shine dalgarno sequence aggaggt.
the shine dalgarno is placed 6 bp from the startcodon (optimal distance) and kozak is 4 of theese bp right before start codon.
This antitoxin is found in E. coli and the sequence is derived from NCBI
antitoxin ccdA working by inhibitting the toxin ccdB (BBa_K899007)
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1