BBa_K899006
type: ComponentDefinition
persistent identity: BBa K899006
display id: BBa_K899006
version: 1
was generated by: iGEM to SBOL conversion
description: heat inducible FFT with TA system compatible with BBa_K899005
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:42Z
mutable provenance: mixed
owned by: james
owned by: myers
mutable description: Heat induced fructan fructan fructosyltransferase (FFT) gene with toxin mazF and antitoxin ccdA (compatible with the TA system of BBa_K899005)
discontinued: false
dominant: false
experience: experience/None
group u list: _1164_
m user id: 0
owner id: 12807
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: none
star: false
creator: Andreas Kj??r
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_K899008
component: BBa_B0034
component: BBa_K338001
component: BBa_K302033
component: BBa_K899001
component: BBa_K899009
sequence annotation: BBa_K338001
sequence annotation: BBa_K899009
sequence annotation: BBa_K899001
sequence annotation: BBa_B0034
sequence annotation: BBa_K302033
sequence annotation: BBa_K899008
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K899006_sequence/1

BBa_B0015
type: Component
persistent identity: component2202827
display id: component2202827
version: 1
top level: BBa_K899006
definition: BBa_B0015
access: public

BBa_K899008
type: Component
persistent identity: component2202820
display id: component2202820
version: 1
top level: BBa_K899006
definition: BBa_K899008
access: public

BBa_B0034
type: Component
persistent identity: component2202819
display id: component2202819
version: 1
top level: BBa_K899006
definition: BBa_B0034
access: public

BBa_K338001
type: Component
persistent identity: component2202807
display id: component2202807
version: 1
top level: BBa_K899006
definition: HSP
access: public

BBa_K302033
type: Component
persistent identity: component2202817
display id: component2202817
version: 1
top level: BBa_K899006
definition: BBa_K302033
access: public

BBa_B0034
type: Component
persistent identity: component2202815
display id: component2202815
version: 1
top level: BBa_K899006
definition: BBa_B0034
access: public

BBa_K899001
type: Component
persistent identity: component2202813
display id: component2202813
version: 1
top level: BBa_K899006
definition: BBa_K899001
access: public

BBa_K899009
type: Component
persistent identity: component2202810
display id: component2202810
version: 1
top level: BBa_K899006
definition: BBa_K899009
access: public

BBa_K338001
type: SequenceAnnotation
persistent identity: annotation2202807
display id: annotation2202807
version: 1
top level: BBa_K899006
location: https://synbiohub.org/public/igem/BBa_K899006/annotation2202807/range2202807/1
component: BBa_K338001

https://synbiohub.org/public/igem/BBa_K899006/annotation2202807/range2202807/1
type: Range
persistent identity: range2202807
display id: range2202807
version: 1
top level: BBa_K899006
direction: direction/reverse
start: 1
end: 98
orientation: inline

BBa_K899009
type: SequenceAnnotation
persistent identity: annotation2202810
display id: annotation2202810
version: 1
top level: BBa_K899006
location: https://synbiohub.org/public/igem/BBa_K899006/annotation2202810/range2202810/1
component: BBa_K899009

https://synbiohub.org/public/igem/BBa_K899006/annotation2202810/range2202810/1
type: Range
persistent identity: range2202810
display id: range2202810
version: 1
top level: BBa_K899006
direction: direction/reverse
start: 107
end: 119
orientation: inline

BBa_K899001
type: SequenceAnnotation
persistent identity: annotation2202813
display id: annotation2202813
version: 1
top level: BBa_K899006
location: https://synbiohub.org/public/igem/BBa_K899006/annotation2202813/range2202813/1
component: BBa_K899001

https://synbiohub.org/public/igem/BBa_K899006/annotation2202813/range2202813/1
type: Range
persistent identity: range2202813
display id: range2202813
version: 1
top level: BBa_K899006
direction: direction/reverse
start: 126
end: 1973
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2202815
display id: annotation2202815
version: 1
top level: BBa_K899006
location: https://synbiohub.org/public/igem/BBa_K899006/annotation2202815/range2202815/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K899006/annotation2202815/range2202815/1
type: Range
persistent identity: range2202815
display id: range2202815
version: 1
top level: BBa_K899006
direction: direction/reverse
start: 1982
end: 1993
orientation: inline

BBa_K302033
type: SequenceAnnotation
persistent identity: annotation2202817
display id: annotation2202817
version: 1
top level: BBa_K899006
location: https://synbiohub.org/public/igem/BBa_K899006/annotation2202817/range2202817/1
component: BBa_K302033

https://synbiohub.org/public/igem/BBa_K899006/annotation2202817/range2202817/1
type: Range
persistent identity: range2202817
display id: range2202817
version: 1
top level: BBa_K899006
direction: direction/reverse
start: 2000
end: 2338
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2202819
display id: annotation2202819
version: 1
top level: BBa_K899006
location: https://synbiohub.org/public/igem/BBa_K899006/annotation2202819/range2202819/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K899006/annotation2202819/range2202819/1
type: Range
persistent identity: range2202819
display id: range2202819
version: 1
top level: BBa_K899006
direction: direction/reverse
start: 2347
end: 2358
orientation: inline

BBa_K899008
type: SequenceAnnotation
persistent identity: annotation2202820
display id: annotation2202820
version: 1
top level: BBa_K899006
location: https://synbiohub.org/public/igem/BBa_K899006/annotation2202820/range2202820/1
component: BBa_K899008

https://synbiohub.org/public/igem/BBa_K899006/annotation2202820/range2202820/1
type: Range
persistent identity: range2202820
display id: range2202820
version: 1
top level: BBa_K899006
direction: direction/reverse
start: 2365
end: 2583
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2202827
display id: annotation2202827
version: 1
top level: BBa_K899006
location: https://synbiohub.org/public/igem/BBa_K899006/annotation2202827/range2202827/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K899006/annotation2202827/range2202827/1
type: Range
persistent identity: range2202827
display id: range2202827
version: 1
top level: BBa_K899006
direction: direction/reverse
start: 2592
end: 2720
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

HSP
type: ComponentDefinition
persistent identity: BBa K338001
display id: BBa_K338001
version: 1
was generated by: iGEM to SBOL conversion
description: Heat Shock Promoter (HSP)
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:07Z
mutable provenance: Modified BBa_K112400
owned by: james
owned by: myers
mutable description: This is a modified K112400 heat shock promoter in BBa standard.
discontinued: false
dominant: false
experience: experience/Works
group u list: _454_
m user id: 0
owner id: 6189
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: None
star: false
creator: 2010 Caltech iGEM Team
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K338001_sequence/1

BBa_K302033
type: ComponentDefinition
persistent identity: BBa K302033
display id: BBa_K302033
version: 1
was generated by: iGEM to SBOL conversion
description: mazF
created: 2010-10-24T11:00:00Z
modified: 2015-06-01T02:27:54Z
mutable provenance: ''Bacillus Subtilis'' 168 strain
owned by: james
owned by: myers
mutable description: Encodes a stable non-specific ribonuclease in ''Bacillus Subtilis''. It is used in conjungtion with ''mazE'' in ''Bacillus Subtilis'' to provide a toxin-antitoxin in various stressful conditions. When transcribtion of both genes is turned off (as both are under contol of same promoter) ''mazE'' will be degraded faster than ''mazF''. There is then no inhibiton of ''mazF'', killing the cell.
discontinued: false
dominant: false
experience: experience/None
group u list: _442_
m user id: 4206
owner id: 6345
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Sequence isolated based upon the work of Pellegrini, O., Mathy, N., Gogos, A., Shapiro, L., and Condon, C. (2005) The ''Bacillus subtilis'' ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor. ''Mol Microbiol'' 56: 1139???1148.
star: false
creator: Philip Hall
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: double TAA added
sequence: https://synbiohub.org/public/igem/BBa_K302033_sequence/1

double TAA added
type: SequenceAnnotation
persistent identity: annotation2099798
display id: annotation2099798
version: 1
top level: BBa_K302033
location: https://synbiohub.org/public/igem/BBa_K302033/annotation2099798/range2099798/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K302033/annotation2099798/range2099798/1
type: Range
persistent identity: range2099798
display id: range2099798
version: 1
top level: BBa_K302033
direction: direction/forward
start: 334
end: 339
orientation: inline

BBa_K899001
type: ComponentDefinition
persistent identity: BBa K899001
display id: BBa_K899001
version: 1
was generated by: iGEM to SBOL conversion
description: Fructan fructan fructosyltransferase (FFT)
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:42Z
mutable provenance: Extracted from Jesrusalem Artichoke (helianthus tuberosus)
owned by: james
owned by: myers
mutable description: Elongates a trisaccharide with the fructose unit of a sucrose substrate into an inulin polymer. Works together with part BBa_K899000 (SST).
discontinued: false
dominant: false
experience: experience/None
group u list: _1164_
m user id: 0
owner id: 12807
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: 7 BP from start codon we embedded an shine dalgarno sequence.
star: false
creator: Andreas Kj??r
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K899001_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2193672
display id: annotation2193672
version: 1
top level: BBa_K899001
location: https://synbiohub.org/public/igem/BBa_K899001/annotation2193672/range2193672/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K899001/annotation2193672/range2193672/1
type: Range
persistent identity: range2193672
display id: range2193672
version: 1
top level: BBa_K899001
direction: direction/forward
start: 1
end: 3
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2193673
display id: annotation2193673
version: 1
top level: BBa_K899001
location: https://synbiohub.org/public/igem/BBa_K899001/annotation2193673/range2193673/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K899001/annotation2193673/range2193673/1
type: Range
persistent identity: range2193673
display id: range2193673
version: 1
top level: BBa_K899001
direction: direction/forward
start: 1846
end: 1848
orientation: inline

BBa_K899009
type: ComponentDefinition
persistent identity: BBa K899009
display id: BBa_K899009
version: 1
was generated by: iGEM to SBOL conversion
description: Doubble ribosomal binding site (FFT) (shine-dalgarno and kozak sequence)
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:42Z
mutable provenance: jerusalem artichoke (Helianthus Tuberosus) and modified with bacterial shine-dalgarno sequence
owned by: james
owned by: myers
mutable description: This is the ribosomal binding site(s) we used in front of our fructan:fructan fructosyltransferase (FFT. It consists of the kozak sequence originally from the gene when we extracted it from the jerusalem artichoke (Helianthus Tuberosus), and the shine dalgarno sequence aggaggt.
discontinued: false
dominant: false
experience: experience/None
group u list: _1164_
m user id: 0
owner id: 12807
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: the shine dalgarno is placed 6 bp from the startcodon (optimal distance) and kozak is 4 of theese bp right before start codon.
star: false
creator: Andreas Kj??r
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Shine-Dalgarno sequence
sequence annotation: Kozak sequence
sequence: https://synbiohub.org/public/igem/BBa_K899009_sequence/1

Shine-Dalgarno sequence
type: SequenceAnnotation
persistent identity: annotation2201693
display id: annotation2201693
version: 1
top level: BBa_K899009
location: https://synbiohub.org/public/igem/BBa_K899009/annotation2201693/range2201693/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K899009/annotation2201693/range2201693/1
type: Range
persistent identity: range2201693
display id: range2201693
version: 1
top level: BBa_K899009
direction: direction/forward
start: 1
end: 7
orientation: inline

Kozak sequence
type: SequenceAnnotation
persistent identity: annotation2201692
display id: annotation2201692
version: 1
top level: BBa_K899009
location: https://synbiohub.org/public/igem/BBa_K899009/annotation2201692/range2201692/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K899009/annotation2201692/range2201692/1
type: Range
persistent identity: range2201692
display id: range2201692
version: 1
top level: BBa_K899009
direction: direction/forward
start: 10
end: 13
orientation: inline

BBa_K899008
type: ComponentDefinition
persistent identity: BBa K899008
display id: BBa_K899008
version: 1
was generated by: iGEM to SBOL conversion
description: antitoxin ccdA
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:42Z
mutable provenance: This antitoxin is found in E. coli and the sequence is derived from NCBI
owned by: james
owned by: myers
mutable description: antitoxin ccdA working by inhibitting the toxin ccdB (BBa_K899007)
discontinued: false
dominant: false
group u list: _1164_
m user id: 0
owner id: 12807
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Andreas Kj??r
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K899008_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K899006_sequence/1
type: Sequence
persistent identity: BBa K899006 sequence
display id: BBa_K899006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccgaggtccttgttgcgaagattgatgacaatgtgagtgcttcccttgaaaccctgaaactgatccccataataagcgaagttagcgagatgaatgcgtactagagaggaggtgtcacctactagatgcaaacccctgaaccctttacagaccttgaacatgaaccccacacacccctactggaccaccaccacaacccaccaccacaaaccaccacaaaacctttgttcaccagggttgtgtccggtgtcacctttgttttattcttctttggtttcgctatcgtattcattgttctcaaccaacagaactcttctgttcgtatcgtcaccaattcggagaaatcttttataaggtattcgcagaccgatcgcttgtcgtgggaacggaccgcttttcattttcagcctgccaagaattttatttacgatccagatggtcagttgtttcacatgggctggtaccatatgttctatcaatacaacccatacgcaccggtttggggcaatatgtcatggggtcactcagtgtccaaagacatgatcaactggtacgagctgccagtcgctatggtcccgaccgaatggtatgatatcgagggcgtcttatccgggtctaccacggtccttccaaacggtcagatctttgcattgtatactgggaacgctaatgatttttcccaattacaatgcaaagctgtacccgtaaacttatctgacccgcttcttattgagtgggtcaagtatgaggataacccaatcctgtacactccaccagggattgggttaaaggactatcgggacccgtcaacagtctggacaggtcccgatggaaagcataggatgatcatgggaactaaacgtggcaatacaggcatggtacttgtttactataccactgattacacgaactacgagttgttggatgagccgttgcactctgttcccaacaccgatatgtgggaatgcgtcgacttttacccggtttcgttaaccaatgatagtgcacttgatatggcggcctatgggtcgggtatcaaacacgttattaaagaaagttgggagggacatggaatggattggtattcaatcgggacatatgacgcgataaatgataaatggactcccgataacccggaactagatgtcggtatcgggttacggtgcgattacgggaggttttttgcatcaaagagtctttatgacccattgaagaaaaggaggatcacttggggttatgttggagaatcagatagtgctgatcaggacctctcaagaggatgggctactgtttataatgttggaagaacaattgtactagatagaaagaccgggacccatttacttcattggcccgttgaggaagtcgagagtttgagatacaacggtcaggagtttaaagagatcaagctagagcccggttcaatcattccactcgacataggcacggctacacagttggacatagttgcaacatttgaggtggatcaagcagcgttgaacgcgacaagtgaaaccgatgatatttatggttgcaccactagcttaggtgcagcccaaaggggaagtttgggaccatttggtcttgcggttctagccgatggaaccctttctgagttaactccggtttatttctatatagctaaaaaggcagatggaggtgtgtcgacacatttttgtaccgataagctaaggtcatcactagattatgatggggagagagtggtgtatgggggcactgttcctgtgttagatgatgaagaactcacaatgaggctattggtggatcattcgatagtggaggggtttgcgcaaggaggaaggacggttataacatcaagggcgtatccaacaaaagcgatatacgaacaagcgaagctgttcttgttcaacaacgccacaggtacgagtgtgaaagcatctctcaagatttggcaaatggcttctgcaccaattcatcaataccctttttaatactagagaaagaggagaaatactaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K338001_sequence/1
type: Sequence
persistent identity: BBa K338001 sequence
display id: BBa_K338001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccgaggtccttgttgcgaagattgatgacaatgtgagtgcttcccttgaaaccctgaaactgatccccataataagcgaagttagcgagatgaatgcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K302033_sequence/1
type: Sequence
persistent identity: BBa K302033 sequence
display id: BBa_K302033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtaagccgatacgtacccgatatgggcgatctgatttgggttgattttgacccgacaaaaggtagcgagcaagctggacatcgtccagctgttgtcctgagtcctttcatgtacaacaacaaaacaggtatgtgtctgtgtgttccttgtacaacgcaatcaaaaggatatccgttcgaagttgttttatccggtcaggaacgtgatggcgtagcgttagctgatcaggtaaaaagtatcgcctggcgggcaagaggagcaacgaagaaaggaacagttgccccagaggaattacaactcattaaagccaaaattaacgtactgattgggtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K899001_sequence/1
type: Sequence
persistent identity: BBa K899001 sequence
display id: BBa_K899001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaaacccctgaaccctttacagaccttgaacatgaaccccacacacccctactggaccaccaccacaacccaccaccacaaaccaccacaaaacctttgttcaccagggttgtgtccggtgtcacctttgttttattcttctttggtttcgctatcgtattcattgttctcaaccaacagaactcttctgttcgtatcgtcaccaattcggagaaatcttttataaggtattcgcagaccgatcgcttgtcgtgggaacggaccgcttttcattttcagcctgccaagaattttatttacgatccagatggtcagttgtttcacatgggctggtaccatatgttctatcaatacaacccatacgcaccggtttggggcaatatgtcatggggtcactcagtgtccaaagacatgatcaactggtacgagctgccagtcgctatggtcccgaccgaatggtatgatatcgagggcgtcttatccgggtctaccacggtccttccaaacggtcagatctttgcattgtatactgggaacgctaatgatttttcccaattacaatgcaaagctgtacccgtaaacttatctgacccgcttcttattgagtgggtcaagtatgaggataacccaatcctgtacactccaccagggattgggttaaaggactatcgggacccgtcaacagtctggacaggtcccgatggaaagcataggatgatcatgggaactaaacgtggcaatacaggcatggtacttgtttactataccactgattacacgaactacgagttgttggatgagccgttgcactctgttcccaacaccgatatgtgggaatgcgtcgacttttacccggtttcgttaaccaatgatagtgcacttgatatggcggcctatgggtcgggtatcaaacacgttattaaagaaagttgggagggacatggaatggattggtattcaatcgggacatatgacgcgataaatgataaatggactcccgataacccggaactagatgtcggtatcgggttacggtgcgattacgggaggttttttgcatcaaagagtctttatgacccattgaagaaaaggaggatcacttggggttatgttggagaatcagatagtgctgatcaggacctctcaagaggatgggctactgtttataatgttggaagaacaattgtactagatagaaagaccgggacccatttacttcattggcccgttgaggaagtcgagagtttgagatacaacggtcaggagtttaaagagatcaagctagagcccggttcaatcattccactcgacataggcacggctacacagttggacatagttgcaacatttgaggtggatcaagcagcgttgaacgcgacaagtgaaaccgatgatatttatggttgcaccactagcttaggtgcagcccaaaggggaagtttgggaccatttggtcttgcggttctagccgatggaaccctttctgagttaactccggtttatttctatatagctaaaaaggcagatggaggtgtgtcgacacatttttgtaccgataagctaaggtcatcactagattatgatggggagagagtggtgtatgggggcactgttcctgtgttagatgatgaagaactcacaatgaggctattggtggatcattcgatagtggaggggtttgcgcaaggaggaaggacggttataacatcaagggcgtatccaacaaaagcgatatacgaacaagcgaagctgttcttgttcaacaacgccacaggtacgagtgtgaaagcatctctcaagatttggcaaatggcttctgcaccaattcatcaataccctttttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K899009_sequence/1
type: Sequence
persistent identity: BBa K899009 sequence
display id: BBa_K899009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggaggtgtcacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K899008_sequence/1
type: Sequence
persistent identity: BBa K899008 sequence
display id: BBa_K899008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaagcagcgcattacagtcaccgtcgacagtgacagctatcagttgctcaaggcatatgatgtcaatatctccggtctggtaagcaccaccatgcagaatgaagcccgtcgtctgcgtgccgaacgctggaaagcagaaaatcaggaagggatggctgaggtcgcccggtttattgaaatgaacggctcttttgccgatgagaacagggactggtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z