BBa_K899000 1 BBa_K899000 Sucrose sucrose fructosyltransferase (SST) 2012-09-21T11:00:00Z 2015-05-08T01:13:42Z Extracted from Jerusalem artichoke (Helianthus tuberosus). Catalyzes sucrose to the three products: D-sucrose, beta-D-fructofuranosyl-(2->1) and alpha-D-glucopyranoside, as such: 2 sucrose -> D-glucose + beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl + alpha-D-glucopyranoside. false false _1164_ 0 12807 9 It's complicated false We removed illigal restriction sites; we added a Shinedalgarno sequence 7 bp from the coding part, to insure expression, while keeping the Kozak sequence to ensure that the part can still be expressed in plants. false Andreas Kj??r annotation2193663 1 Start codon range2193663 1 1 3 annotation2193664 1 Stop codon range2193664 1 1891 1893 BBa_K899010 1 BBa_K899010 Doubble ribosomal binding site (SST) (shine-dalgarno and kozak sequence) 2012-09-25T11:00:00Z 2015-05-08T01:13:42Z Kozak sequence derives from jerusalem artichoke (helianthus tuberosus) shinedalgarno was introduced by primers. This sequence is taken from the RBS section of our sucrose:sucrose fructosyltransferase (SST) gene. It consists of a shine dalgarno sequence (AGGAGGT) and a kozak sequence (CACC). false false _1164_ 0 12807 9 Not in stock false none false Andreas Kj??r annotation2201790 1 Kozak sequence range2201790 1 10 13 annotation2201787 1 Shine-Dalgarno sequence range2201787 1 1 7 BBa_K899012 1 BBa_K899012 SST (BBa_K899001) with double RBS (BBa_K899010) 2012-09-25T11:00:00Z 2015-05-08T01:13:42Z none none false false _1164_ 0 12807 9 Not in stock false none false Andreas Kj??r component2202323 1 BBa_K899000 component2202320 1 BBa_K899010 annotation2202323 1 BBa_K899000 range2202323 1 20 1912 annotation2202320 1 BBa_K899010 range2202320 1 1 13 BBa_K899000_sequence 1 atgatggcttcatccaccaccaccacccctctcattctccatgatgaccctgaaaacctcccagaactcaccggttctccgacaactcgtcgtctatccatcgcaaaagtgctttcggggatccttgtttcggttctggttataggtgctcttgttgctttaatcaacaaccaaacatatgaatccccctcggccaccacattcgtaactcagttgccaaatattgatctgaagcgggttccaggaaagttggattcgagtgctgaggttgaatggcaacgatccacttatcattttcaacccgacaaaaatttcattagcgatcctgatggcccaatgtatcacatgggatggtatcatctattttatcagtacaaccctcaatctgccatctggggcaacatcacatggggccactcggtatcgaaagacatgatcaactggttccatctccctttcgccatggttcctgaccattggtacgacatcgaaggtgtcatgacgggttcggctacagtcctccctaatggtcaaatcatcatgctttactcgggcaacgcgtatgatctctcccaagtacaatgcttggcgtacgctgtcaactcgtcggatccacttcttatagagtggaaaaaatatgaaggtaaccctgtcttactcccaccaccaggagtaggctacaaggactttcgggacccatccacattgtggtcgggccctgatggtgaatatagaatggtaatggggtccaagcacaacgagactattggctgtgctttgatttaccataccactaattttacgcattttgaattgaaagaggaggtgcttcatgcagtcccacatactggtatgtgggaatgtgttgatctttacccggtgtccaccgtacacacaaacgggctggacatggtggataacgggccaaatgttaagtacgtgttgaaacaaagtggggatgaagatcgccatgattggtatgcaattggaagttacgatatagtgaatgataagtggtacccagatgacccggaaaatgatgtgggtatcggattaagatatgattttggaaaattttatgcgtccaagacgttttatgaccaacataagaagaggagagtcctttggggctatgttggagaaaccgatccccaaaagtatgacctttcaaagggatgggctaacattttgaatattccaaggaccgtcgttttggacctcgaaactaaaaccaatttgattcaatggccaatcgaggaaaccgaaaaccttaggtcgaaaaagtatgatgaatttaaagacgtcgagcttcgacccggggcactcgttccccttgagataggcacagccacacagttggatatagttgcgacattcgaaatcgaccaaaagatgttggaatcaacgctagaggccgatgttctattcaattgcacgacaagtgaaggctcggttgcaaggagtgtgttgggaccgtttggtgtggtggttctagccgatgcccagcgctccgaacaacttcctgtatacttctatatcgcaaaagatattgatggaacctcacgaacttatttttgtgccgacgaaacaagatcatccaaggatgtaagcgtagggaaatgggtgtacggaagcagtgttcctgtcctcccaggcgaaaagtacaatatgaggttattggtggatcattcgatagtagagggatttgcacaaaacgggagaaccgtggtgacatcaagagtgtatccaacaaaggcgatctacaacgctgcgaaggtgtttttgttcaacaacgcgactggaatcagtgtgaaggcgtcgatcaagatctggaagatgggggaagcagaactcaatcctttccctcttcctgggtggactttcgaactttga BBa_K899012_sequence 1 aggaggttacacctactagatgatggcttcatccaccaccaccacccctctcattctccatgatgaccctgaaaacctcccagaactcaccggttctccgacaactcgtcgtctatccatcgcaaaagtgctttcggggatccttgtttcggttctggttataggtgctcttgttgctttaatcaacaaccaaacatatgaatccccctcggccaccacattcgtaactcagttgccaaatattgatctgaagcgggttccaggaaagttggattcgagtgctgaggttgaatggcaacgatccacttatcattttcaacccgacaaaaatttcattagcgatcctgatggcccaatgtatcacatgggatggtatcatctattttatcagtacaaccctcaatctgccatctggggcaacatcacatggggccactcggtatcgaaagacatgatcaactggttccatctccctttcgccatggttcctgaccattggtacgacatcgaaggtgtcatgacgggttcggctacagtcctccctaatggtcaaatcatcatgctttactcgggcaacgcgtatgatctctcccaagtacaatgcttggcgtacgctgtcaactcgtcggatccacttcttatagagtggaaaaaatatgaaggtaaccctgtcttactcccaccaccaggagtaggctacaaggactttcgggacccatccacattgtggtcgggccctgatggtgaatatagaatggtaatggggtccaagcacaacgagactattggctgtgctttgatttaccataccactaattttacgcattttgaattgaaagaggaggtgcttcatgcagtcccacatactggtatgtgggaatgtgttgatctttacccggtgtccaccgtacacacaaacgggctggacatggtggataacgggccaaatgttaagtacgtgttgaaacaaagtggggatgaagatcgccatgattggtatgcaattggaagttacgatatagtgaatgataagtggtacccagatgacccggaaaatgatgtgggtatcggattaagatatgattttggaaaattttatgcgtccaagacgttttatgaccaacataagaagaggagagtcctttggggctatgttggagaaaccgatccccaaaagtatgacctttcaaagggatgggctaacattttgaatattccaaggaccgtcgttttggacctcgaaactaaaaccaatttgattcaatggccaatcgaggaaaccgaaaaccttaggtcgaaaaagtatgatgaatttaaagacgtcgagcttcgacccggggcactcgttccccttgagataggcacagccacacagttggatatagttgcgacattcgaaatcgaccaaaagatgttggaatcaacgctagaggccgatgttctattcaattgcacgacaagtgaaggctcggttgcaaggagtgtgttgggaccgtttggtgtggtggttctagccgatgcccagcgctccgaacaacttcctgtatacttctatatcgcaaaagatattgatggaacctcacgaacttatttttgtgccgacgaaacaagatcatccaaggatgtaagcgtagggaaatgggtgtacggaagcagtgttcctgtcctcccaggcgaaaagtacaatatgaggttattggtggatcattcgatagtagagggatttgcacaaaacgggagaaccgtggtgacatcaagagtgtatccaacaaaggcgatctacaacgctgcgaaggtgtttttgttcaacaacgcgactggaatcagtgtgaaggcgtcgatcaagatctggaagatgggggaagcagaactcaatcctttccctcttcctgggtggactttcgaactttga BBa_K899010_sequence 1 aggaggttacacc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z