LOCUS       BBa_K901003             1959 bp    DNA     linear       22-Dec-2024
DEFINITION  InvA Expression Construct
ACCESSION   BBa_K901003
VERSION     BBa_K901003.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..1777
                     /label=invA
     CDS             227..1783
                     /label=BBa_K901000
     stem_loop       1803..1846
                     /label=stem_loop
     misc_feature    1792..1871
                     /label=BBa_B0010
     terminator      1792..1871
                     /label=BBa_B0010
     stem_loop       1891..1934
                     /label=stem_loop
     misc_feature    1880..1959
                     /label=BBa_B0010
     terminator      1880..1959
                     /label=BBa_B0010
     terminator      1792..1959
                     /label=BBa_B0017
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ctagtgaaag
      241 ccccagctac aaaaacctga tcaaagccga agatgcccag aagaaagccg gcaaacgcct
      301 gctgagcagc gaatggtacc ccggctttca cgtgaccccc ctgaccggct ggatgaacga
      361 tcccaacggc ctgatctttt tcaaaggcga ataccacctg ttttaccagt actacccctt
      421 tgctccagtg tggggtccaa tgcactgggg tcacgctaaa agccgcgatc tggtgcactg
      481 ggaaaccctg ccagtggccc tggcccccgg cgatagcttt gatcgcgatg gctgctttag
      541 cggctgcgcc gtggataaca acggcgtgct gaccctgatc tacaccggcc acatcgtgct
      601 gagcaacgat agcctggatg ccatccgcga agtgcagtgc atggcgacca gcatcgatgg
      661 catccacttt cagaaagaag gcatcgtgct ggaaaaagcc cccatgcccc aggtggccca
      721 ctttcgcgat ccccgcgtgt ggaaagaaaa caaccactgg tttatggtgg tgggctaccg
      781 caccgatgat gaaaaacacc agggcatcgg ccacgtggcc ctgtaccgca gcgaaaacct
      841 gaaagattgg atctttgtga aaaccctgct gggcgataac agccagctgc ccctgggcaa
      901 acgcgccttt atgtgggaat gccccgattt ctttagcctg ggcaaccgca gcgtgctgat
      961 gtttagcccc cagggcctga aagccagcgg ctacaaaaac cgcaacctgt ttcagaacgg
     1021 ctacatcctg ggcaaatggc aggcccccca gtttaccccc gaaaccagct ttcaggaact
     1081 ggattacggc cacgattttt acgccgccca gcgctttgaa gccaaagatg gccgccagat
     1141 cctgatcgcc tggtttgata tgtgggaaaa ccagaaaccc agccagcgcg atggctgggc
     1201 cggctgcatg accctgcccc gcaaactgga tctgatcgat aacaaaatcg tgatgacccc
     1261 cgtgcgcgaa atggaaatcc tgcgccagag cgaaaagatc gaaagcgtgg tgaccctgag
     1321 cgatgccgaa caccccttta ccatggatag ccccctgcaa gaaatcgaac tgatctttga
     1381 tctggaaaag agcagcgctt accaggctgg cctggccctg cgctgcaacg gtaagggtca
     1441 ggaaaccctg ctgtacatcg atcgcagcca gaaccgcatc atcctggatc gcaaccgcag
     1501 cggccagaac gtgaaaggca tccgcagctg ccccctgccc aacaccagca aagtgcgcct
     1561 gcacatcttt ctggatcgca gcagcatcga aatctttgtg ggcgatgatc agacccaggg
     1621 cctgtacagc atcagcagcc gcatctttcc cgataaagat agcctgaaag gccgcctgtt
     1681 tgccatcgaa ggctacgccg tgtttgatag ctttaaacgc tggaccctgc aagatgccaa
     1741 cctggccgcc tttagcagcg atgcctgcgg cagcagctaa taatactaga gccaggcatc
     1801 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1861 tgaacgctct ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1921 gcctttcgtt ttatctgttg tttgtcggtg aacgctctc
//