LOCUS       BBa_K901007             1314 bp    DNA     linear       29-May-2024
DEFINITION  GalU Expression Construct
ACCESSION   BBa_K901007
VERSION     BBa_K901007.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..1132
                     /label=galU
     CDS             227..1138
                     /label=BBa_K901004
     stem_loop       1158..1201
                     /label=stem_loop
     misc_feature    1147..1226
                     /label=BBa_B0010
     terminator      1147..1226
                     /label=BBa_B0010
     stem_loop       1246..1289
                     /label=stem_loop
     misc_feature    1235..1314
                     /label=BBa_B0010
     terminator      1235..1314
                     /label=BBa_B0010
     terminator      1147..1314
                     /label=BBa_B0017
     misc_feature    227..1314
                     /label=BBa_K901005
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ccgccatcaa
      241 caccaaggtg aagaaggccg tgatccccgt ggccggcctg ggcacccgca tgctgcccgc
      301 caccaaggcc atccccaagg agatgctgcc cctggtggat aagcccctga tccagtacgt
      361 ggtgaacgag tgcatcgccg ccggcatcac cgagatcgtg ctggtgaccc acagcagcaa
      421 gaacagcatc gagaaccact tcgataccag cttcgagctg gaggccatgc tggagaagcg
      481 cgtgaagcgc cagctgctgg atgaggtgca gagcatctgc cccccccacg tgaccatcat
      541 gcaggtgcgc cagggcctgg ccaagggcct gggccacgcc gtgctgtgcg cccaccccgt
      601 ggtgggcgat gagcccgtgg ccgtgatcct gcccgatgtg atcctggatg agtacgagag
      661 cgatctgagc caggataacc tggccgagat gatccgccgc ttcgatgaga ccggccacag
      721 ccagatcatg gtggagcccg tggccgatgt gaccgcctac ggcgtggtgg attgcaaggg
      781 cgtggagctg gcccccggcg agagcgtgcc catggtgggc gtggtggaga agcccaaggc
      841 cgatgtggcc cccagcaacc tggccatcgt gggccgctac gtgctgagcg ccgatatctg
      901 gcccctgctg gccaagaccc cccccggcgc cggcgatgag atccagctga ccgatgccat
      961 cgatatgctg atcgagaagg agaccgtgga ggcctaccac atgaagggca agagccacga
     1021 ttgcggcaac aagctgggct acatgcaggc cttcgtggag tacggcatcc gccacaacac
     1081 cctgggcacc gagttcaagg cctggctgga ggaggagatg ggcatcaaga agtaataata
     1141 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1201 tctgttgttt gtcggtgaac gctctctact agagccaggc atcaaataaa acgaaaggct
     1261 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctc
//