LOCUS BBa_K901011 1955 bp DNA linear 25-Nov-2024 DEFINITION AatA with a double terminator ACCESSION BBa_K901011 VERSION BBa_K901011.1 FEATURES Location/Qualifiers CDS 1..1776 /label=aatA CDS 1..1779 /label=BBa_K901009 stem_loop 1799..1842 /label=stem_loop misc_feature 1788..1867 /label=BBa_B0010 terminator 1788..1867 /label=BBa_B0010 stem_loop 1887..1930 /label=stem_loop misc_feature 1876..1955 /label=BBa_B0010 terminator 1876..1955 /label=BBa_B0010 terminator 1788..1955 /label=BBa_B0017 ORIGIN 1 atggcgcatc cgccgctgct gcatctgcaa gatattaccc tgagcctggg cggcaacccg 61 ctgctggatg gcgcgggctt tgcggtgggc cgcggcgaac gcctgtgcct ggtgggccgc 121 aacggcagcg gcaaaagcac cctgctgaaa attgcggcgg gcgtgattca gccggatagc 181 ggcagcgtgt ttgtgcagcc gggcgcgagc ctgcgctatc tgccgcagga accggatctg 241 agcgcgtatg cgaccaccgc ggattatgtg gtgggccaga ttggcgatcc ggatatggcg 301 tggcgcgcga ccccgctgct ggatgcgctg ggcctgaccg gccgcgaaag cacccagaac 361 ctgagcggcg gcgaaggccg ccgctgcgcg attgcgggcg tgctggcggc ggcgccggat 421 gtgctgctgc tggatgaacc gaccaaccat ctggatatgc cgaccattga atggctggaa 481 cgcgaactgc tgagcctggg cgcgatggtg attattagcc atgatcgccg cctgctgagc 541 accctgagcc gcagcgtggt gtggctggat cgcggcgtga cccgccgcct ggatgaaggc 601 tttggccgct ttgaagcgtg gcgcgaagaa gtgctggaac aggaagaacg cgatgcgcat 661 aaactggatc gcaaaattgc gcgcgaagaa gattggatgc gctatggcgt gaccgcgcgc 721 cgcaaacgca acgtgcgccg cgtgcgcgaa ctggcggatc tgcgcaccgc gcgcaaagaa 781 gcgattcgcg cgccgggcac cctgaccctg aacacccagc tgcgcccgca tcgcaaactg 841 gtggcggtgg cggaagatat tagcaaagcg tggggcgaaa aacaggtggt gcgccatctg 901 gatctgcgca ttctgcgcgg cgatcgcctg ggcattgtgg gcgcgaacgg cgcgggcaaa 961 accaccctgc tgcgcatgct gaccggcctg gatcagccgg atagcggcac cattagcctg 1021 ggcccgagcc tgaacatggt gaccctggat cagcagcgcc gcaccctgaa cccggaacgc 1081 accctggcgg ataccctgac cgaaggcggc ggcgatatgg tgcaggtggg caccgaaaaa 1141 cgccatgtgg tgggctatat gaaagatttt ctgtttcgcc cggaacaggc gcgcaccccg 1201 gtgagcgcgc tgagcggcgg cgaacgtggc cgcctgatgc tggcgtgcgc gctggcgaaa 1261 ccgagcaacc tgctggtgct ggatgaaccg accaacgatc tggatctgga aaccctggat 1321 attctgcaag atatgctggc gagctgcgaa ggcaccgtgc tgctggtgag ccatgatcgc 1381 gattttctgg atcgcgtggc gaccagcgtg ctggcgaccg aaggcgatgg caactggatt 1441 gaatatgcgg gcggctatag cgatatgctg gcgcagcgcc atcagaaacc gctgaccacc 1501 gcgagcgtgg tggaaaacga accgaccaaa ccgaaagaaa ccaccgcggc gcgcggcccg 1561 accaaaaaac tgagctataa agatcagttt gcgctggata acctgccgaa agaaatggaa 1621 aaactggaag cgcaggcggc gaactgcgtg aaaaactggc agattcagat ttatatggaa 1681 aaaaccccgc gcagcctgcg caactttcgc ctgatttatc gcagcagcaa acagagctgg 1741 cagaacctga aaaacgcggg ctggaactgg aaataataat actagagcca ggcatcaaat 1801 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 1861 cgctctctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 1921 ttcgttttat ctgttgtttg tcggtgaacg ctctc // LOCUS BBa_K901012 2181 bp DNA linear 25-Nov-2024 DEFINITION AatA Expression Construct ACCESSION BBa_K901012 VERSION BBa_K901012.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 1..220 /label=BBa_J04500 CDS 227..2002 /label=aatA CDS 227..2005 /label=BBa_K901009 stem_loop 2025..2068 /label=stem_loop misc_feature 2014..2093 /label=BBa_B0010 terminator 2014..2093 /label=BBa_B0010 stem_loop 2113..2156 /label=stem_loop misc_feature 2102..2181 /label=BBa_B0010 terminator 2102..2181 /label=BBa_B0010 terminator 2014..2181 /label=BBa_B0017 misc_feature 227..2181 /label=BBa_K901010 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cgcatccgcc 241 gctgctgcat ctgcaagata ttaccctgag cctgggcggc aacccgctgc tggatggcgc 301 gggctttgcg gtgggccgcg gcgaacgcct gtgcctggtg ggccgcaacg gcagcggcaa 361 aagcaccctg ctgaaaattg cggcgggcgt gattcagccg gatagcggca gcgtgtttgt 421 gcagccgggc gcgagcctgc gctatctgcc gcaggaaccg gatctgagcg cgtatgcgac 481 caccgcggat tatgtggtgg gccagattgg cgatccggat atggcgtggc gcgcgacccc 541 gctgctggat gcgctgggcc tgaccggccg cgaaagcacc cagaacctga gcggcggcga 601 aggccgccgc tgcgcgattg cgggcgtgct ggcggcggcg ccggatgtgc tgctgctgga 661 tgaaccgacc aaccatctgg atatgccgac cattgaatgg ctggaacgcg aactgctgag 721 cctgggcgcg atggtgatta ttagccatga tcgccgcctg ctgagcaccc tgagccgcag 781 cgtggtgtgg ctggatcgcg gcgtgacccg ccgcctggat gaaggctttg gccgctttga 841 agcgtggcgc gaagaagtgc tggaacagga agaacgcgat gcgcataaac tggatcgcaa 901 aattgcgcgc gaagaagatt ggatgcgcta tggcgtgacc gcgcgccgca aacgcaacgt 961 gcgccgcgtg cgcgaactgg cggatctgcg caccgcgcgc aaagaagcga ttcgcgcgcc 1021 gggcaccctg accctgaaca cccagctgcg cccgcatcgc aaactggtgg cggtggcgga 1081 agatattagc aaagcgtggg gcgaaaaaca ggtggtgcgc catctggatc tgcgcattct 1141 gcgcggcgat cgcctgggca ttgtgggcgc gaacggcgcg ggcaaaacca ccctgctgcg 1201 catgctgacc ggcctggatc agccggatag cggcaccatt agcctgggcc cgagcctgaa 1261 catggtgacc ctggatcagc agcgccgcac cctgaacccg gaacgcaccc tggcggatac 1321 cctgaccgaa ggcggcggcg atatggtgca ggtgggcacc gaaaaacgcc atgtggtggg 1381 ctatatgaaa gattttctgt ttcgcccgga acaggcgcgc accccggtga gcgcgctgag 1441 cggcggcgaa cgtggccgcc tgatgctggc gtgcgcgctg gcgaaaccga gcaacctgct 1501 ggtgctggat gaaccgacca acgatctgga tctggaaacc ctggatattc tgcaagatat 1561 gctggcgagc tgcgaaggca ccgtgctgct ggtgagccat gatcgcgatt ttctggatcg 1621 cgtggcgacc agcgtgctgg cgaccgaagg cgatggcaac tggattgaat atgcgggcgg 1681 ctatagcgat atgctggcgc agcgccatca gaaaccgctg accaccgcga gcgtggtgga 1741 aaacgaaccg accaaaccga aagaaaccac cgcggcgcgc ggcccgacca aaaaactgag 1801 ctataaagat cagtttgcgc tggataacct gccgaaagaa atggaaaaac tggaagcgca 1861 ggcggcgaac tgcgtgaaaa actggcagat tcagatttat atggaaaaaa ccccgcgcag 1921 cctgcgcaac tttcgcctga tttatcgcag cagcaaacag agctggcaga acctgaaaaa 1981 cgcgggctgg aactggaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc 2041 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 2101 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 2161 tgtttgtcgg tgaacgctct c //