BBa_K901012 1 BBa_K901012 AatA Expression Construct 2012-09-30T11:00:00Z 2015-05-08T01:13:42Z J04500 K901010 pLacI regulate AatA with double terminator false false _1166_ 0 6015 9 Not in stock false Regulated construct for Acetic acid transport A expression false Anthony Vuong component2205580 1 BBa_J04500 component2205587 1 BBa_K901010 annotation2205580 1 BBa_J04500 range2205580 1 1 220 annotation2205587 1 BBa_K901010 range2205587 1 227 2181 BBa_K901011 1 BBa_K901011 AatA with a double terminator 2012-09-30T11:00:00Z 2015-05-08T01:13:42Z K901009 B0017 Acetic acid transporter with double terminator false true _1166_ 0 13351 9 Not in stock false Intermediate false Iain Barkley component2205704 1 BBa_B0017 component2205699 1 BBa_K901009 annotation2205699 1 BBa_K901009 range2205699 1 1 1779 annotation2205704 1 BBa_B0017 range2205704 1 1788 1955 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K901009 1 AatA AatA from <i>Acetobacter acetii</i> 2012-09-30T11:00:00Z 2015-05-08T01:13:42Z <i>Escherichia coli</i> Acetic acid transport A. An ABC transport protein. false false _1166_ 0 6015 9 Not in stock false Transport of acetic acid false Anthony Vuong annotation2205706 1 aatA range2205706 1 1 1776 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961225 1 -10 range1961225 1 161 166 annotation1961224 1 -35 range1961224 1 137 142 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961227 1 start range1961227 1 173 173 annotation1961226 1 LacI binding site range1961226 1 166 200 BBa_J04500 1 BBa_J04500 IPTG inducible promoter with RBS 2005-06-08T11:00:00Z 2015-08-31T04:08:14Z Davidson Synth-Aces Released HQ 2013 R0010.B0034 false true _16_ 0 326 16 In stock false false Kristen DeCelle component1508159 1 BBa_B0034 component1508149 1 BBa_R0010 annotation1508159 1 BBa_B0034 range1508159 1 209 220 annotation1508149 1 BBa_R0010 range1508149 1 1 200 BBa_B0017 1 BBa_B0017 double terminator (B0010-B0010) 2004-01-08T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator with two copies of <bb_part>BBa_B0010</bb_part> false false _1_ 0 24 7 In stock false true Austin Che component939337 1 BBa_B0010 component939331 1 BBa_B0010 annotation939331 1 BBa_B0010 range939331 1 1 80 annotation939337 1 BBa_B0010 range939337 1 89 168 BBa_K901010 1 BBa_K901010 AatA with dT 2012-09-30T11:00:00Z 2015-05-08T01:13:42Z K901009 with B0017 AatA with a double terminator at the 3' end true false _1166_ 0 6015 9 Discontinued false Intermediate construct false Anthony Vuong component2205566 1 BBa_K901009 component2205571 1 BBa_B0017 annotation2205566 1 BBa_K901009 range2205566 1 1 1779 annotation2205571 1 BBa_B0017 range2205571 1 1788 1955 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K901012_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcgcatccgccgctgctgcatctgcaagatattaccctgagcctgggcggcaacccgctgctggatggcgcgggctttgcggtgggccgcggcgaacgcctgtgcctggtgggccgcaacggcagcggcaaaagcaccctgctgaaaattgcggcgggcgtgattcagccggatagcggcagcgtgtttgtgcagccgggcgcgagcctgcgctatctgccgcaggaaccggatctgagcgcgtatgcgaccaccgcggattatgtggtgggccagattggcgatccggatatggcgtggcgcgcgaccccgctgctggatgcgctgggcctgaccggccgcgaaagcacccagaacctgagcggcggcgaaggccgccgctgcgcgattgcgggcgtgctggcggcggcgccggatgtgctgctgctggatgaaccgaccaaccatctggatatgccgaccattgaatggctggaacgcgaactgctgagcctgggcgcgatggtgattattagccatgatcgccgcctgctgagcaccctgagccgcagcgtggtgtggctggatcgcggcgtgacccgccgcctggatgaaggctttggccgctttgaagcgtggcgcgaagaagtgctggaacaggaagaacgcgatgcgcataaactggatcgcaaaattgcgcgcgaagaagattggatgcgctatggcgtgaccgcgcgccgcaaacgcaacgtgcgccgcgtgcgcgaactggcggatctgcgcaccgcgcgcaaagaagcgattcgcgcgccgggcaccctgaccctgaacacccagctgcgcccgcatcgcaaactggtggcggtggcggaagatattagcaaagcgtggggcgaaaaacaggtggtgcgccatctggatctgcgcattctgcgcggcgatcgcctgggcattgtgggcgcgaacggcgcgggcaaaaccaccctgctgcgcatgctgaccggcctggatcagccggatagcggcaccattagcctgggcccgagcctgaacatggtgaccctggatcagcagcgccgcaccctgaacccggaacgcaccctggcggataccctgaccgaaggcggcggcgatatggtgcaggtgggcaccgaaaaacgccatgtggtgggctatatgaaagattttctgtttcgcccggaacaggcgcgcaccccggtgagcgcgctgagcggcggcgaacgtggccgcctgatgctggcgtgcgcgctggcgaaaccgagcaacctgctggtgctggatgaaccgaccaacgatctggatctggaaaccctggatattctgcaagatatgctggcgagctgcgaaggcaccgtgctgctggtgagccatgatcgcgattttctggatcgcgtggcgaccagcgtgctggcgaccgaaggcgatggcaactggattgaatatgcgggcggctatagcgatatgctggcgcagcgccatcagaaaccgctgaccaccgcgagcgtggtggaaaacgaaccgaccaaaccgaaagaaaccaccgcggcgcgcggcccgaccaaaaaactgagctataaagatcagtttgcgctggataacctgccgaaagaaatggaaaaactggaagcgcaggcggcgaactgcgtgaaaaactggcagattcagatttatatggaaaaaaccccgcgcagcctgcgcaactttcgcctgatttatcgcagcagcaaacagagctggcagaacctgaaaaacgcgggctggaactggaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0017_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K901011_sequence 1 atggcgcatccgccgctgctgcatctgcaagatattaccctgagcctgggcggcaacccgctgctggatggcgcgggctttgcggtgggccgcggcgaacgcctgtgcctggtgggccgcaacggcagcggcaaaagcaccctgctgaaaattgcggcgggcgtgattcagccggatagcggcagcgtgtttgtgcagccgggcgcgagcctgcgctatctgccgcaggaaccggatctgagcgcgtatgcgaccaccgcggattatgtggtgggccagattggcgatccggatatggcgtggcgcgcgaccccgctgctggatgcgctgggcctgaccggccgcgaaagcacccagaacctgagcggcggcgaaggccgccgctgcgcgattgcgggcgtgctggcggcggcgccggatgtgctgctgctggatgaaccgaccaaccatctggatatgccgaccattgaatggctggaacgcgaactgctgagcctgggcgcgatggtgattattagccatgatcgccgcctgctgagcaccctgagccgcagcgtggtgtggctggatcgcggcgtgacccgccgcctggatgaaggctttggccgctttgaagcgtggcgcgaagaagtgctggaacaggaagaacgcgatgcgcataaactggatcgcaaaattgcgcgcgaagaagattggatgcgctatggcgtgaccgcgcgccgcaaacgcaacgtgcgccgcgtgcgcgaactggcggatctgcgcaccgcgcgcaaagaagcgattcgcgcgccgggcaccctgaccctgaacacccagctgcgcccgcatcgcaaactggtggcggtggcggaagatattagcaaagcgtggggcgaaaaacaggtggtgcgccatctggatctgcgcattctgcgcggcgatcgcctgggcattgtgggcgcgaacggcgcgggcaaaaccaccctgctgcgcatgctgaccggcctggatcagccggatagcggcaccattagcctgggcccgagcctgaacatggtgaccctggatcagcagcgccgcaccctgaacccggaacgcaccctggcggataccctgaccgaaggcggcggcgatatggtgcaggtgggcaccgaaaaacgccatgtggtgggctatatgaaagattttctgtttcgcccggaacaggcgcgcaccccggtgagcgcgctgagcggcggcgaacgtggccgcctgatgctggcgtgcgcgctggcgaaaccgagcaacctgctggtgctggatgaaccgaccaacgatctggatctggaaaccctggatattctgcaagatatgctggcgagctgcgaaggcaccgtgctgctggtgagccatgatcgcgattttctggatcgcgtggcgaccagcgtgctggcgaccgaaggcgatggcaactggattgaatatgcgggcggctatagcgatatgctggcgcagcgccatcagaaaccgctgaccaccgcgagcgtggtggaaaacgaaccgaccaaaccgaaagaaaccaccgcggcgcgcggcccgaccaaaaaactgagctataaagatcagtttgcgctggataacctgccgaaagaaatggaaaaactggaagcgcaggcggcgaactgcgtgaaaaactggcagattcagatttatatggaaaaaaccccgcgcagcctgcgcaactttcgcctgatttatcgcagcagcaaacagagctggcagaacctgaaaaacgcgggctggaactggaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J04500_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa BBa_K901009_sequence 1 atggcgcatccgccgctgctgcatctgcaagatattaccctgagcctgggcggcaacccgctgctggatggcgcgggctttgcggtgggccgcggcgaacgcctgtgcctggtgggccgcaacggcagcggcaaaagcaccctgctgaaaattgcggcgggcgtgattcagccggatagcggcagcgtgtttgtgcagccgggcgcgagcctgcgctatctgccgcaggaaccggatctgagcgcgtatgcgaccaccgcggattatgtggtgggccagattggcgatccggatatggcgtggcgcgcgaccccgctgctggatgcgctgggcctgaccggccgcgaaagcacccagaacctgagcggcggcgaaggccgccgctgcgcgattgcgggcgtgctggcggcggcgccggatgtgctgctgctggatgaaccgaccaaccatctggatatgccgaccattgaatggctggaacgcgaactgctgagcctgggcgcgatggtgattattagccatgatcgccgcctgctgagcaccctgagccgcagcgtggtgtggctggatcgcggcgtgacccgccgcctggatgaaggctttggccgctttgaagcgtggcgcgaagaagtgctggaacaggaagaacgcgatgcgcataaactggatcgcaaaattgcgcgcgaagaagattggatgcgctatggcgtgaccgcgcgccgcaaacgcaacgtgcgccgcgtgcgcgaactggcggatctgcgcaccgcgcgcaaagaagcgattcgcgcgccgggcaccctgaccctgaacacccagctgcgcccgcatcgcaaactggtggcggtggcggaagatattagcaaagcgtggggcgaaaaacaggtggtgcgccatctggatctgcgcattctgcgcggcgatcgcctgggcattgtgggcgcgaacggcgcgggcaaaaccaccctgctgcgcatgctgaccggcctggatcagccggatagcggcaccattagcctgggcccgagcctgaacatggtgaccctggatcagcagcgccgcaccctgaacccggaacgcaccctggcggataccctgaccgaaggcggcggcgatatggtgcaggtgggcaccgaaaaacgccatgtggtgggctatatgaaagattttctgtttcgcccggaacaggcgcgcaccccggtgagcgcgctgagcggcggcgaacgtggccgcctgatgctggcgtgcgcgctggcgaaaccgagcaacctgctggtgctggatgaaccgaccaacgatctggatctggaaaccctggatattctgcaagatatgctggcgagctgcgaaggcaccgtgctgctggtgagccatgatcgcgattttctggatcgcgtggcgaccagcgtgctggcgaccgaaggcgatggcaactggattgaatatgcgggcggctatagcgatatgctggcgcagcgccatcagaaaccgctgaccaccgcgagcgtggtggaaaacgaaccgaccaaaccgaaagaaaccaccgcggcgcgcggcccgaccaaaaaactgagctataaagatcagtttgcgctggataacctgccgaaagaaatggaaaaactggaagcgcaggcggcgaactgcgtgaaaaactggcagattcagatttatatggaaaaaaccccgcgcagcctgcgcaactttcgcctgatttatcgcagcagcaaacagagctggcagaacctgaaaaacgcgggctggaactggaaataataa BBa_K901010_sequence 1 atggcgcatccgccgctgctgcatctgcaagatattaccctgagcctgggcggcaacccgctgctggatggcgcgggctttgcggtgggccgcggcgaacgcctgtgcctggtgggccgcaacggcagcggcaaaagcaccctgctgaaaattgcggcgggcgtgattcagccggatagcggcagcgtgtttgtgcagccgggcgcgagcctgcgctatctgccgcaggaaccggatctgagcgcgtatgcgaccaccgcggattatgtggtgggccagattggcgatccggatatggcgtggcgcgcgaccccgctgctggatgcgctgggcctgaccggccgcgaaagcacccagaacctgagcggcggcgaaggccgccgctgcgcgattgcgggcgtgctggcggcggcgccggatgtgctgctgctggatgaaccgaccaaccatctggatatgccgaccattgaatggctggaacgcgaactgctgagcctgggcgcgatggtgattattagccatgatcgccgcctgctgagcaccctgagccgcagcgtggtgtggctggatcgcggcgtgacccgccgcctggatgaaggctttggccgctttgaagcgtggcgcgaagaagtgctggaacaggaagaacgcgatgcgcataaactggatcgcaaaattgcgcgcgaagaagattggatgcgctatggcgtgaccgcgcgccgcaaacgcaacgtgcgccgcgtgcgcgaactggcggatctgcgcaccgcgcgcaaagaagcgattcgcgcgccgggcaccctgaccctgaacacccagctgcgcccgcatcgcaaactggtggcggtggcggaagatattagcaaagcgtggggcgaaaaacaggtggtgcgccatctggatctgcgcattctgcgcggcgatcgcctgggcattgtgggcgcgaacggcgcgggcaaaaccaccctgctgcgcatgctgaccggcctggatcagccggatagcggcaccattagcctgggcccgagcctgaacatggtgaccctggatcagcagcgccgcaccctgaacccggaacgcaccctggcggataccctgaccgaaggcggcggcgatatggtgcaggtgggcaccgaaaaacgccatgtggtgggctatatgaaagattttctgtttcgcccggaacaggcgcgcaccccggtgagcgcgctgagcggcggcgaacgtggccgcctgatgctggcgtgcgcgctggcgaaaccgagcaacctgctggtgctggatgaaccgaccaacgatctggatctggaaaccctggatattctgcaagatatgctggcgagctgcgaaggcaccgtgctgctggtgagccatgatcgcgattttctggatcgcgtggcgaccagcgtgctggcgaccgaaggcgatggcaactggattgaatatgcgggcggctatagcgatatgctggcgcagcgccatcagaaaccgctgaccaccgcgagcgtggtggaaaacgaaccgaccaaaccgaaagaaaccaccgcggcgcgcggcccgaccaaaaaactgagctataaagatcagtttgcgctggataacctgccgaaagaaatggaaaaactggaagcgcaggcggcgaactgcgtgaaaaactggcagattcagatttatatggaaaaaaccccgcgcagcctgcgcaactttcgcctgatttatcgcagcagcaaacagagctggcagaacctgaaaaacgcgggctggaactggaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z