LOCUS       BBa_K901015             1608 bp    DNA     linear       22-Nov-2024
DEFINITION  AcK Expression Construct
ACCESSION   BBa_K901015
VERSION     BBa_K901015.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..1432
                     /label=ackA
     CDS             227..1432
                     /label=BBa_K901013
     stem_loop       1452..1495
                     /label=stem_loop
     misc_feature    1441..1520
                     /label=BBa_B0010
     terminator      1441..1520
                     /label=BBa_B0010
     stem_loop       1540..1583
                     /label=stem_loop
     misc_feature    1529..1608
                     /label=BBa_B0010
     terminator      1529..1608
                     /label=BBa_B0010
     terminator      1441..1608
                     /label=BBa_B0017
     misc_feature    227..1608
                     /label=BBa_K901014
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gcagcaaact
      241 ggtgctggtg ctgaactgcg gcagcagcag cctgaaattt gcgattattg atgcggtgaa
      301 cggcgaagaa tatctgagcg gcctggcgga atgctttcat ctgccggaag cgcgcattaa
      361 atggaaaatg gatggcaaca aacaggaagc ggcgctgggc gcgggcgcgg cgcatagcga
      421 agcgctgaac tttattgtga acaccattct ggcgcagaaa ccggaactga gcgcgcagct
      481 gaccgcgatt ggccatcgca ttgtgcatgg cggcgaaaaa tataccagca gcgtggtgat
      541 tgatgaaagc gtgattcagg gcattaaaga tgcggcgagc tttgcgccgc tgcataaccc
      601 ggcgcatctg attggcattg aagaagcgct gaaaagcttt ccgcagctga aagataaaaa
      661 cgtggcggtg tttgataccg cgtttcatca gaccatgccg gaagaaagct atctgtatgc
      721 gctgccgtat aacctgtata aagaacatgg cattcgccgc tatggcgcgc atggcaccag
      781 ccatttttat gtgacccagg aagcggcgaa aatgctgaac aaaccggtgg aagaactgaa
      841 cattattacc tgccatctgg gcaacggcgg cagcgtgagc gcgattcgca acggcaaatg
      901 cgtggatacc agcatgggcc tgaccccgct ggaaggcctg gtgatgggca cccgcagcgg
      961 cgatattgat ccggcgatta tttttcatct gcatgatacc ctgggcatga gcgtggatgc
     1021 gattaacaaa ctgctgacca aagaaagcgg cctgctgggc ctgaccgaag tgaccagcga
     1081 ttgccgctat gtggaagata actatgcgac caaagaagat gcgaaacgcg cgatggatgt
     1141 gtattgccat cgcctggcga aatatattgg cgcgtatacc gcgctgatgg atggccgcct
     1201 ggatgcggtg gtgtttaccg gcggcattgg cgaaaacgcg gcgatggtgc gcgaactgag
     1261 cctgggcaaa ctgggcgtgc tgggctttga agtggatcat gaacgcaacc tggcggcgcg
     1321 ctttggcaaa agcggcttta ttaacaaaga aggcacccgc ccggcggtgg tgattccgac
     1381 caacgaagaa ctggtgattg cgcaggatgc gagccgcctg accgcgtaat aatactagag
     1441 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1501 gtttgtcggt gaacgctctc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1561 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctc
//