BBa_K901015 1 BBa_K901015 AcK Expression Construct 2012-09-30T11:00:00Z 2015-05-08T01:13:42Z This part is made from BBa_J04500 and BBa_K901014. This is the full construct for acetate kinase. false false _1166_ 0 13351 9 It's complicated true Full Construct false Iain Barkley component2205750 1 BBa_K901014 component2205742 1 BBa_J04500 annotation2205742 1 BBa_J04500 range2205742 1 1 220 annotation2205750 1 BBa_K901014 range2205750 1 227 1608 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961225 1 -10 range1961225 1 161 166 annotation1961227 1 start range1961227 1 173 173 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 BBa_J04500 1 BBa_J04500 IPTG inducible promoter with RBS 2005-06-08T11:00:00Z 2015-08-31T04:08:14Z Davidson Synth-Aces Released HQ 2013 R0010.B0034 false true _16_ 0 326 16 In stock false false Kristen DeCelle component1508149 1 BBa_R0010 component1508159 1 BBa_B0034 annotation1508159 1 BBa_B0034 range1508159 1 209 220 annotation1508149 1 BBa_R0010 range1508149 1 1 200 BBa_B0017 1 BBa_B0017 double terminator (B0010-B0010) 2004-01-08T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator with two copies of <bb_part>BBa_B0010</bb_part> false false _1_ 0 24 7 In stock false true Austin Che component939337 1 BBa_B0010 component939331 1 BBa_B0010 annotation939331 1 BBa_B0010 range939331 1 1 80 annotation939337 1 BBa_B0010 range939337 1 89 168 BBa_K901013 1 AcK Acetate Kinase (AcK) from <i>Escherichia coli</i> 2012-09-30T11:00:00Z 2015-05-08T01:13:42Z <i>Escherichia coli</i> Acetate kinase adds phosphate to acetate as a step in transportation of acetate out the the cell. false false _1166_ 0 6015 9 Not in stock false Phosphorylates acetate. false Anthony Vuong annotation2205705 1 ackA range2205705 1 1 1206 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K901014 1 BBa_K901014 AcK with double terminator 2012-10-01T11:00:00Z 2015-05-08T01:13:42Z Ack I'm sorry false false _1166_ 0 13351 9 Not in stock false intermediate false Iain Barkley component2205740 1 BBa_B0017 component2205730 1 BBa_K901013 annotation2205730 1 BBa_K901013 range2205730 1 1 1206 annotation2205740 1 BBa_B0017 range2205740 1 1215 1382 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K901015_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgagcagcaaactggtgctggtgctgaactgcggcagcagcagcctgaaatttgcgattattgatgcggtgaacggcgaagaatatctgagcggcctggcggaatgctttcatctgccggaagcgcgcattaaatggaaaatggatggcaacaaacaggaagcggcgctgggcgcgggcgcggcgcatagcgaagcgctgaactttattgtgaacaccattctggcgcagaaaccggaactgagcgcgcagctgaccgcgattggccatcgcattgtgcatggcggcgaaaaatataccagcagcgtggtgattgatgaaagcgtgattcagggcattaaagatgcggcgagctttgcgccgctgcataacccggcgcatctgattggcattgaagaagcgctgaaaagctttccgcagctgaaagataaaaacgtggcggtgtttgataccgcgtttcatcagaccatgccggaagaaagctatctgtatgcgctgccgtataacctgtataaagaacatggcattcgccgctatggcgcgcatggcaccagccatttttatgtgacccaggaagcggcgaaaatgctgaacaaaccggtggaagaactgaacattattacctgccatctgggcaacggcggcagcgtgagcgcgattcgcaacggcaaatgcgtggataccagcatgggcctgaccccgctggaaggcctggtgatgggcacccgcagcggcgatattgatccggcgattatttttcatctgcatgataccctgggcatgagcgtggatgcgattaacaaactgctgaccaaagaaagcggcctgctgggcctgaccgaagtgaccagcgattgccgctatgtggaagataactatgcgaccaaagaagatgcgaaacgcgcgatggatgtgtattgccatcgcctggcgaaatatattggcgcgtataccgcgctgatggatggccgcctggatgcggtggtgtttaccggcggcattggcgaaaacgcggcgatggtgcgcgaactgagcctgggcaaactgggcgtgctgggctttgaagtggatcatgaacgcaacctggcggcgcgctttggcaaaagcggctttattaacaaagaaggcacccgcccggcggtggtgattccgaccaacgaagaactggtgattgcgcaggatgcgagccgcctgaccgcgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0017_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_J04500_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa BBa_K901013_sequence 1 atgagcagcaaactggtgctggtgctgaactgcggcagcagcagcctgaaatttgcgattattgatgcggtgaacggcgaagaatatctgagcggcctggcggaatgctttcatctgccggaagcgcgcattaaatggaaaatggatggcaacaaacaggaagcggcgctgggcgcgggcgcggcgcatagcgaagcgctgaactttattgtgaacaccattctggcgcagaaaccggaactgagcgcgcagctgaccgcgattggccatcgcattgtgcatggcggcgaaaaatataccagcagcgtggtgattgatgaaagcgtgattcagggcattaaagatgcggcgagctttgcgccgctgcataacccggcgcatctgattggcattgaagaagcgctgaaaagctttccgcagctgaaagataaaaacgtggcggtgtttgataccgcgtttcatcagaccatgccggaagaaagctatctgtatgcgctgccgtataacctgtataaagaacatggcattcgccgctatggcgcgcatggcaccagccatttttatgtgacccaggaagcggcgaaaatgctgaacaaaccggtggaagaactgaacattattacctgccatctgggcaacggcggcagcgtgagcgcgattcgcaacggcaaatgcgtggataccagcatgggcctgaccccgctggaaggcctggtgatgggcacccgcagcggcgatattgatccggcgattatttttcatctgcatgataccctgggcatgagcgtggatgcgattaacaaactgctgaccaaagaaagcggcctgctgggcctgaccgaagtgaccagcgattgccgctatgtggaagataactatgcgaccaaagaagatgcgaaacgcgcgatggatgtgtattgccatcgcctggcgaaatatattggcgcgtataccgcgctgatggatggccgcctggatgcggtggtgtttaccggcggcattggcgaaaacgcggcgatggtgcgcgaactgagcctgggcaaactgggcgtgctgggctttgaagtggatcatgaacgcaacctggcggcgcgctttggcaaaagcggctttattaacaaagaaggcacccgcccggcggtggtgattccgaccaacgaagaactggtgattgcgcaggatgcgagccgcctgaccgcgtaataa BBa_K901014_sequence 1 atgagcagcaaactggtgctggtgctgaactgcggcagcagcagcctgaaatttgcgattattgatgcggtgaacggcgaagaatatctgagcggcctggcggaatgctttcatctgccggaagcgcgcattaaatggaaaatggatggcaacaaacaggaagcggcgctgggcgcgggcgcggcgcatagcgaagcgctgaactttattgtgaacaccattctggcgcagaaaccggaactgagcgcgcagctgaccgcgattggccatcgcattgtgcatggcggcgaaaaatataccagcagcgtggtgattgatgaaagcgtgattcagggcattaaagatgcggcgagctttgcgccgctgcataacccggcgcatctgattggcattgaagaagcgctgaaaagctttccgcagctgaaagataaaaacgtggcggtgtttgataccgcgtttcatcagaccatgccggaagaaagctatctgtatgcgctgccgtataacctgtataaagaacatggcattcgccgctatggcgcgcatggcaccagccatttttatgtgacccaggaagcggcgaaaatgctgaacaaaccggtggaagaactgaacattattacctgccatctgggcaacggcggcagcgtgagcgcgattcgcaacggcaaatgcgtggataccagcatgggcctgaccccgctggaaggcctggtgatgggcacccgcagcggcgatattgatccggcgattatttttcatctgcatgataccctgggcatgagcgtggatgcgattaacaaactgctgaccaaagaaagcggcctgctgggcctgaccgaagtgaccagcgattgccgctatgtggaagataactatgcgaccaaagaagatgcgaaacgcgcgatggatgtgtattgccatcgcctggcgaaatatattggcgcgtataccgcgctgatggatggccgcctggatgcggtggtgtttaccggcggcattggcgaaaacgcggcgatggtgcgcgaactgagcctgggcaaactgggcgtgctgggctttgaagtggatcatgaacgcaacctggcggcgcgctttggcaaaagcggctttattaacaaagaaggcacccgcccggcggtggtgattccgaccaacgaagaactggtgattgcgcaggatgcgagccgcctgaccgcgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z