LOCUS       BBa_K901019             2550 bp    DNA     linear       25-Nov-2024
DEFINITION  PTA Expression Construct
ACCESSION   BBa_K901019
VERSION     BBa_K901019.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..2374
                     /label=pta
     CDS             227..2374
                     /label=BBa_K901016
     stem_loop       2394..2437
                     /label=stem_loop
     misc_feature    2383..2462
                     /label=BBa_B0010
     terminator      2383..2462
                     /label=BBa_B0010
     stem_loop       2482..2525
                     /label=stem_loop
     misc_feature    2471..2550
                     /label=BBa_B0010
     terminator      2471..2550
                     /label=BBa_B0010
     terminator      2383..2550
                     /label=BBa_B0017
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gccgcattat
      241 tatgctgatt ccgaccggca ccagcgtggg cctgaccagc gtgagcctgg gcgtgattcg
      301 cgcgatggaa cgcaaaggcg tgcgcctgag cgtgtttaaa ccgattgcgc agccgcgcac
      361 cggcggcgat gcgccggatc agaccaccac cattgtgcgc gcgaacagca gcaccaccac
      421 cgcggcggaa ccgctgaaaa tgagctatgt ggaaggcctg ctgagcagca accagaaaga
      481 tgtgctgatg gaagaaattg tggcgaacta tcatgcgaac accaaagatg cggaagtggt
      541 gctggtggaa ggcctggtgc cgacccgcaa acatcagttt gcgcagagcc tgaactatga
      601 aattgcgaaa accctgaacg cggaaattgt gtttgtgatg agccagggca ccgatacccc
      661 ggaacagctg aaagaacgca ttgaactgac ccgcaacagc tttggcggcg cgaaaaacac
      721 caacattacc ggcgtgattg tgaacaaact gaacgcgccg gtggatgaac agggccgcac
      781 ccgcccggat ctgagcgaaa tttttgatga tagcagcaaa gcgaaagtga acaacgtgga
      841 tccggcgaaa ctgcaagaaa gcagcccgct gccggtgctg ggcgcggtgc cgtggagctt
      901 tgatctgatt gcgacccgcg cgattgatat ggcgcgccat ctgaacgcga ccattattaa
      961 cgaaggcgat attaacaccc gccgcgtgaa aagcgtgacc ttttgcgcgc gcagcattcc
     1021 gcatatgctg gaacattttc gcgcgggcag cctgctggtg accagcgcgg atcgcccgga
     1081 tgtgctggtg gcggcgtgcc tggcggcgat gaacggcgtg gaaattggcg cgctgctgct
     1141 gaccggcggc tatgaaatgg atgcgcgcat tagcaaactg tgcgaacgcg cgtttgcgac
     1201 cggcctgccg gtgtttatgg tgaacaccaa cacctggcag accagcctga gcctgcaaag
     1261 ctttaacctg gaagtgccgg tggatgatca tgaacgcatt gaaaaagtgc aggaatatgt
     1321 ggcgaactat attaacgcgg attggattga aagcctgacc gcgaccagcg aacgcagccg
     1381 ccgcctgagc ccgccggcgt ttcgctatca gctgaccgaa ctggcgcgca aagcgggcaa
     1441 acgcattgtg ctgccggaag gcgatgaacc gcgcaccgtg aaagcggcgg cgatttgcgc
     1501 ggaacgcggc attgcgacct gcgtgctgct gggcaacccg gcggaaatta accgcgtggc
     1561 ggcgagccag ggcgtggaac tgggcgcggg cattgaaatt gtggatccgg aagtggtgcg
     1621 cgaaagctat gtgggccgcc tggtggaact gcgcaaaaac aaaggcatga ccgaaaccgt
     1681 ggcgcgcgaa cagctggaag ataacgtggt gctgggcacc ctgatgctgg aacaggatga
     1741 agtggatggc ctggtgagcg gcgcggtgca taccaccgcg aacaccattc gcccgccgct
     1801 gcaactgatt aaaaccgcgc cgggcagcag cctggtgagc agcgtgtttt ttatgctgct
     1861 gccggaacag gtgtatgtgt atggcgattg cgcgattaac ccggatccga ccgcggaaca
     1921 gctggcggaa attgcgattc agagcgcgga tagcgcggcg gcgtttggca ttgaaccgcg
     1981 cgtggcgatg ctgagctata gcaccggcac cagcggcgcg ggcagcgatg tggaaaaagt
     2041 gcgcgaagcg acccgcctgg cgcaggaaaa acgcccggat ctgatgattg atggcccgct
     2101 gcaatatgat gcggcggtga tggcggatgt ggcgaaaagc aaagcgccga acagcccggt
     2161 ggcgggccgc gcgaccgtgt ttatttttcc ggatctgaac accggcaaca ccacctataa
     2221 agcggtgcag cgcagcgcgg atctgattag cattggcccg atgctgcaag gcatgcgcaa
     2281 accggtgaac gatctgagcc gcggcgcgct ggtggatgat attgtgtata ccattgcgct
     2341 gaccgcgatt cagagcgcgc agcagcagta ataatactag agccaggcat caaataaaac
     2401 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     2461 tctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2521 tttatctgtt gtttgtcggt gaacgctctc
//