LOCUS       BBa_K902002             2038 bp    DNA     linear       17-May-2024
DEFINITION  Beta-glucuronidase (uidA) inducible generator
ACCESSION   BBa_K902002
VERSION     BBa_K902002.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..229
                     /label=start
     misc_feature    2036..2038
                     /label=stop
     CDS             227..2038
                     /label=UidA
     CDS             227..2038
                     /label=BBa_K902000
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt tacgtcctgt
      241 agaaacccca acccgtgaaa tcaaaaaact cgacggcctg tgggcattca gtctggatcg
      301 cgaaaactgt ggaattgatc agcgttggtg ggaaagcgcg ttacaagaaa gccgggcaat
      361 tgctgtgcca ggcagtttta acgatcagtt cgccgatgca gatattcgta attatgcggg
      421 caacgtctgg tatcagcgcg aagtctttat accgaaaggt tgggcaggcc agcgtatcgt
      481 gctgcgtttc gatgcggtca ctcattacgg caaagtgtgg gtcaataatc aggaagtgat
      541 ggagcatcag ggcggctata cgccatttga agccgatgtc acgccgtatg ttattgccgg
      601 gaaaagtgta cgtatcaccg tttgtgtgaa caacgaactg aactggcaga ctatcccgcc
      661 gggaatggtg attaccgacg aaaacggcaa gaaaaagcag tcttacttcc atgatttctt
      721 taactatgcc gggatccatc gcagcgtaat gctctacacc acgccgaaca cctgggtgga
      781 cgatatcacc gtggtgacgc atgtcgcgca agactgtaac cacgcgtctg ttgactggca
      841 ggtggtggcc aatggtgatg tcagcgttga actgcgtgat gcggatcaac aggtggttgc
      901 aactggacaa ggcactagcg ggactttgca agtggtgaat ccgcacctct ggcaaccggg
      961 tgaaggttat ctctatgaac tgtgcgtcac agccaaaagc cagacagagt gtgatatcta
     1021 cccgcttcgc gtcggcatcc ggtcagtggc agtgaagggc gaacagttcc tgattaacca
     1081 caaaccgttc tactttactg gctttggtcg tcatgaagat gcggacttgc gtggcaaagg
     1141 attcgataac gtgctgatgg tgcacgacca cgcattaatg gactggattg gggccaactc
     1201 ctaccgtacc tcgcattacc cttacgctga agagatgctc gactgggcag atgaacatgg
     1261 catcgtggtg attgatgaaa ctgctgctgt cggctttaac ctctctttag gcattggttt
     1321 cgaagcgggc aacaagccga aagaactgta cagcgaagag gcagtcaacg gggaaactca
     1381 gcaagcgcac ttacaggcga ttaaagagct gatagcgcgt gacaaaaacc acccaagcgt
     1441 ggtgatgtgg agtattgcca acgaaccgga tacccgtccg caaggtgcac gggaatattt
     1501 cgcgccactg gcggaagcaa cgcgtaaact cgacccgacg cgtccgatca cctgcgtcaa
     1561 tgtaatgttc tgcgacgctc acaccgatac catcagcgat ctctttgatg tgctgtgcct
     1621 gaaccgttat tacggatggt atgtccaaag cggcgatttg gaaacggcag agaaggtact
     1681 ggaaaaagaa cttctggcct ggcaggagaa actgcatcag ccgattatca tcaccgaata
     1741 cggcgtggat acgttagccg ggctgcactc aatgtacacc gacatgtgga gtgaagagta
     1801 tcagtgtgca tggctggata tgtatcaccg cgtctttgat cgcgtcagcg ccgtcgtcgg
     1861 tgaacaggta tggaatttcg ccgattttgc gacctcgcaa ggcatattgc gcgttggcgg
     1921 taacaagaaa gggatcttca ctcgcgaccg caaaccgaag tcggcggctt ttctgctgca
     1981 aaaacgctgg actggcatga acttcggtga aaaaccgcag cagggaggca aacaatga
//