LOCUS       BBa_K902006             2524 bp    DNA     linear       22-Dec-2024
DEFINITION  Beta-D-glucoside glucohydrolase (bglX) inducible generator
ACCESSION   BBa_K902006
VERSION     BBa_K902006.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..229
                     /label=start
     misc_feature    2522..2524
                     /label=stop
     CDS             227..2524
                     /label=bglX
     CDS             227..2524
                     /label=BBa_K902004
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aatggctatg
      241 ttcagtagga atcgcggtga gtctggccct acagccagca ctggcggatg atttattcgg
      301 caaccatcca ttaacgcccg aagcgcggga tgcgttcgtc accgaactgc ttaagaaaat
      361 gacagttgat gagaaaattg gtcagctgcg cttaatcagc gtcggcccgg ataacccgaa
      421 agaggcgatc cgcgagatga tcaaagacgg tcaggttggg gcgattttca acaccgtaac
      481 ccgtcaggat atccgcgcca tgcaggatca ggtgatggaa ttaagccgcc tgaaaattcc
      541 tcttttcttt gcttacgacg tgctgcacgg tcagcgcacg gtgttcccga ttagcctcgg
      601 tctggcctcg tcttttaacc tcgatgcagt gaaaacggtc ggacgtgtct ctgcttatga
      661 agcggcagat gatggcctga atatgacctg ggcaccgatg gtcgatgtct cgcgcgatcc
      721 gcgctgggga cgtgcttccg aaggttttgg cgaagatacg tatctcacct caacaatggg
      781 taaaaccatg gtggaagcga tgcagggtaa aagcccggca gatcgctact cggtgatgac
      841 cagcgtcaaa cactttgccg catacggcgc ggtagaaggc ggtaaagagt acaacaccgt
      901 cgatatgagt ccgcagcgcc tgtttaatga ttatatgccg ccgtacaaag cggggctgga
      961 cgcaggcagc ggcgcggtga tggtggcgct gaactcgctg aacggcacgc cagccacctc
     1021 cgattcctgg ctgctgaaag atgttctgcg cgaccagtgg ggctttaaag gcatcaccgt
     1081 ttccgatcac ggtgcaatca aagagctgat taaacatggc acggcggcag acccggaaga
     1141 tgcggtgcgc gtggcgctga aatccggaat caacatgagc atgagcgacg agtactactc
     1201 gaagtatctg cctgggttga ttaaatccgg caaagtgacg atggcagagc tggacgatgc
     1261 tgcccgccat gtactgaacg ttaaatatga tatggggttg tttaacgacc catacagcca
     1321 tttggggccg aaagagtctg acccggtgga taccaatgcc gaaagccgcc tgcaccgtaa
     1381 agaagcgcgt gaagtggcgc gcgaaagctt ggtgttgctg aaaaaccgtc tcgaaacgtt
     1441 accgctgaaa aaatcggcca ccattgcggt ggttgggcca ctggcggaca gtaaacgtga
     1501 cgtgatgggc agctggtccg cagccggtgt tgccgatcaa tccgtgaccg tactgaccgg
     1561 gattaaaaat gccgtcggtg aaaacggtaa agtgctgtat gccaaagggg cgaacgttac
     1621 cagtgacaaa ggcattatcg atttcctgaa tcagtatgaa gaagcggtca aagtcgatcc
     1681 gcgttcgccg caagagatga ttgatgaagc ggtgcagacg gcgaaacaat ctgatgtggt
     1741 ggtggctgta gtcggtgaag cacaggggat ggcgcacgaa gcctccagcc ggaccgatat
     1801 cactattccg caaagccaac gtgacttgat tgcggcgctg aaagccaccg gtaaaccgct
     1861 ggtgctggtg ctgatgaacg ggcgtccgct ggcgctggtg aaagaagatc agcaggctga
     1921 tgcgattctg gaaacctggt ttgcggggac tgaaggcggt aatgcaattg ccgatgtatt
     1981 gtttggcgat tacaacccgt ccggcaagct gccaatgtcc ttcccgcgtt ctgtcgggca
     2041 gatcccggtg tactacagcc atctgaatac cggtcgcccg tataatgccg acaagccgaa
     2101 caaatacact tcgcgttatt ttgatgaagc taacggggcg ttgtatccgt tcggctatgg
     2161 gctgagctac accactttca ccgtctctga tgtgaaactt tctgcgccga ccatgaagcg
     2221 tgacggcaaa gtgactgcca gcgtgcaggt gacgaacacc ggtaagcgcg agggtgccac
     2281 ggtagtgcag atgtacttgc aggatgtgac ggcttccatg agtcgccctg tgaaacagct
     2341 gaaaggcttt gagaaaatca ccctgaaacc gggcgaaact cagactgtca gcttcccgat
     2401 cgatattgag gcgctgaagt tctggaatca acagatgaaa tatgacgccg agcctggcaa
     2461 gttcaatgtc tttatcggca ctgattccgc acgcgttaag aaaggcgagt ttgagttgct
     2521 gtaa
//