BBa_K902023 1 BBa_K902023 Moco Riboswitch 2012-10-01T11:00:00Z 2015-05-08T01:13:43Z moco ribo moco ribo false false _1167_ 0 13453 9 In stock false moco ribo false Somshukla Chaudhuri BBa_K902022 1 BBa_K902022 CviAII restriction endonuclease 2012-10-01T11:00:00Z 2015-05-08T01:13:43Z Chlorella virus PCBVI CviAII false false _1167_ 0 13453 9 It's complicated false Kill gene false Himika Dastidar BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961227 1 start range1961227 1 173 173 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 BBa_K902027 1 BBa_K902027 LacI(R0010)-MOCO riboswitch-CviAII 2012-10-01T11:00:00Z 2015-05-08T01:13:43Z LacI(R0010)-MOCO riboswitch-CviAII LacI(R0010)-MOCO riboswitch-CviAII false false _1167_ 0 13453 9 It's complicated false LacI(R0010)-MOCO riboswitch-CviAII false Somshukla Chaudhuri component2207167 1 BBa_K902023 component2207160 1 BBa_R0010 component2207168 1 BBa_K902022 annotation2207168 1 BBa_K902022 range2207168 1 344 1375 annotation2207160 1 BBa_R0010 range2207160 1 1 200 annotation2207167 1 BBa_K902023 range2207167 1 209 337 BBa_K902022_sequence 1 atgacccaaaaaattctaaatccggtgacgggtcgcttcgtgaaggtagacggttcgaccggcaaaaaaatcaaaactgggaacatctacgacgtgaataacattctctcgtccaaactgactaaaaagatctttgataaaatccgacgcaatgatttggctaaagaagaacgtgaaacgcagcaactgaccaagaaaatttctaaaggcattttcgataaaatccgctccgaaaacaaaaaatatgaaaagaatacgcagaagctcacaaagaagatggtgttggacatcttcagtaaaatttataaggaggactcacgtaagagcaagacgcagtgtgtagtttccgaaaaaaaagacaatggcggtgtgcttctgactgaagatctggggaaaatttttgaaaagtcaatatgcatgttgtacgataccccctatatcggtccgtacaagtatggaaacgaaaaacctatgctcctgaagacgcgtcttacaaaattgctggacttcttccctgagctgacacatacggccgcgggtggcgcgctgcatgacttcaccactaaaaacagtcggtatctgagcgccaaaacatcaaagaaaaaagatggaaaagttgcaccacaaaaaattggtcagcccaccaaaaaaaaatttcttgagttctttaatctcccgccggacacgagtaatgatgatatcaaattatttatcaaaaaaaatatagtgcgcattctggatgaatatttcaaatacacctttgatgataccattatctactataacgaagttaataacattatcatgttagtcaaaaccttaaaaaaagtcaagttcgatccgaacttaattgaatttggctgtaacaaaccaggcaaatcttggaaagagagcaccattctgttctacaacaacaaacgcctgggcgagtttcagattcacaccagccgtagttgcattaaattccgttggttttttgagaacatcctgctgctgtttccggataactttgaggtcaccattctgtaataa BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K902023_sequence 1 gggaaccactaaacacctagcctctgcacctgggtcaactgatacggtgctttggccgtgacaatgctcgtaaagattgccaccagggcgaaggaagaaatgacttcgcctcccgtatctggaaaggtg BBa_K902027_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagaggggaaccactaaacacctagcctctgcacctgggtcaactgatacggtgctttggccgtgacaatgctcgtaaagattgccaccagggcgaaggaagaaatgacttcgcctcccgtatctggaaaggtgtactagatgacccaaaaaattctaaatccggtgacgggtcgcttcgtgaaggtagacggttcgaccggcaaaaaaatcaaaactgggaacatctacgacgtgaataacattctctcgtccaaactgactaaaaagatctttgataaaatccgacgcaatgatttggctaaagaagaacgtgaaacgcagcaactgaccaagaaaatttctaaaggcattttcgataaaatccgctccgaaaacaaaaaatatgaaaagaatacgcagaagctcacaaagaagatggtgttggacatcttcagtaaaatttataaggaggactcacgtaagagcaagacgcagtgtgtagtttccgaaaaaaaagacaatggcggtgtgcttctgactgaagatctggggaaaatttttgaaaagtcaatatgcatgttgtacgataccccctatatcggtccgtacaagtatggaaacgaaaaacctatgctcctgaagacgcgtcttacaaaattgctggacttcttccctgagctgacacatacggccgcgggtggcgcgctgcatgacttcaccactaaaaacagtcggtatctgagcgccaaaacatcaaagaaaaaagatggaaaagttgcaccacaaaaaattggtcagcccaccaaaaaaaaatttcttgagttctttaatctcccgccggacacgagtaatgatgatatcaaattatttatcaaaaaaaatatagtgcgcattctggatgaatatttcaaatacacctttgatgataccattatctactataacgaagttaataacattatcatgttagtcaaaaccttaaaaaaagtcaagttcgatccgaacttaattgaatttggctgtaacaaaccaggcaaatcttggaaagagagcaccattctgttctacaacaacaaacgcctgggcgagtttcagattcacaccagccgtagttgcattaaattccgttggttttttgagaacatcctgctgctgtttccggataactttgaggtcaccattctgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z