BBa_K902034 1 BBa_K902034 CarAd Coding Sequence 2012-09-30T11:00:00Z 2015-05-08T01:13:43Z Pseudomonas putida plasmid pCAR1.2 The CarAd gene in Pseudomonas putida codes for the ferredoxin reductase component of carbazole-1,9-dioxygenase. Together with CarAa and CarAc it forms the carbazole-1,9-dioxygenase (CARDO) enzyme which converts carbazole into 2'-aminobiphenyl-2,3-diol by cleaving one C-N bond in the original carbazole molecule. false false _1167_ 0 13454 9 In stock false Site directed mutagenesis was required to remove a NotI site. false Jeff Addison annotation2205697 1 NotI mutation (G --> A) range2205697 1 657 657 annotation2205695 1 ATG Start Codon range2205695 1 1 3 annotation2205696 1 TAA Stop Codon range2205696 1 988 990 BBa_K902034_sequence 1 atgtaccaactcaaaattgaagggcaagcgccagggacctgcggctcagggaagagcctgttggtctcagcacttgctaatggtatcggatttccgtacgagtgtgcatcgggaggttgcggagtatgcaaattcgagttactcgaagggaatgtccaatcaatgtggccggatgctccaggactttcttcgcgagatcgtgagaagggcaaccgccatcttgcatgccagtgcgttgcgctctcagacctgcggatcaaagtcgcagtgcaggacaagtacgtcccaacgattccaatctcaagaatggaagcggaagttgttgaggtccgggcgctaactcatgacctgctgtccgtgcgattacgcactgatgggccagcaaatttcctccccggccagttctgcctagtagaggcagagcagttgccaggcgtggttcgcgcatattcaatggcgaatttaaagaaccccgaaggcatatgggagttctatattaagagggtacccacaggacgatttagtccttggcttttcgaaaatagaaaagaaggcgctcgtctatttttgacgggaccaatgggcacatctttcttccgtccagggaccggccgaaagagtctttgcattggcggcggtgccgggctctcgtatgcagccgctattgcacgcgcctcgatgcgcgaaacagacaagccggtaaagttgttctacggctcaagaactccgcgcgacgctgttcggtggatcgatatcgacatcgatgaggacaagcttgaggtcgtccaggcagttacggaagacacggatagcctttggcaagggcccactggttttattcatcaggttgtcgacgcagcgctgcttgaaaccctaccggaatacgaaatttatcttgccggtccaccgcctatggtcgacgctactgtccgtatgctgctcggcaagggtgttccacgcgatcaaattcattttgacgcatttttctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z