BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K902034 1 BBa_K902034 CarAd Coding Sequence 2012-09-30T11:00:00Z 2015-05-08T01:13:43Z Pseudomonas putida plasmid pCAR1.2 The CarAd gene in Pseudomonas putida codes for the ferredoxin reductase component of carbazole-1,9-dioxygenase. Together with CarAa and CarAc it forms the carbazole-1,9-dioxygenase (CARDO) enzyme which converts carbazole into 2'-aminobiphenyl-2,3-diol by cleaving one C-N bond in the original carbazole molecule. false false _1167_ 0 13454 9 In stock false Site directed mutagenesis was required to remove a NotI site. false Jeff Addison annotation2205695 1 ATG Start Codon range2205695 1 1 3 annotation2205697 1 NotI mutation (G --> A) range2205697 1 657 657 annotation2205696 1 TAA Stop Codon range2205696 1 988 990 BBa_K902040 1 BBa_K902040 B0034 + CarAd 2012-10-01T11:00:00Z 2015-05-08T01:13:43Z no later false false _1167_ 0 13454 9 In stock false none false Jeff Addison, Anya Kornilo component2207194 1 BBa_K902034 component2207190 1 BBa_B0034 annotation2207190 1 BBa_B0034 range2207190 1 1 12 annotation2207194 1 BBa_K902034 range2207194 1 19 1008 BBa_B0034_sequence 1 aaagaggagaaa BBa_K902040_sequence 1 aaagaggagaaatactagatgtaccaactcaaaattgaagggcaagcgccagggacctgcggctcagggaagagcctgttggtctcagcacttgctaatggtatcggatttccgtacgagtgtgcatcgggaggttgcggagtatgcaaattcgagttactcgaagggaatgtccaatcaatgtggccggatgctccaggactttcttcgcgagatcgtgagaagggcaaccgccatcttgcatgccagtgcgttgcgctctcagacctgcggatcaaagtcgcagtgcaggacaagtacgtcccaacgattccaatctcaagaatggaagcggaagttgttgaggtccgggcgctaactcatgacctgctgtccgtgcgattacgcactgatgggccagcaaatttcctccccggccagttctgcctagtagaggcagagcagttgccaggcgtggttcgcgcatattcaatggcgaatttaaagaaccccgaaggcatatgggagttctatattaagagggtacccacaggacgatttagtccttggcttttcgaaaatagaaaagaaggcgctcgtctatttttgacgggaccaatgggcacatctttcttccgtccagggaccggccgaaagagtctttgcattggcggcggtgccgggctctcgtatgcagccgctattgcacgcgcctcgatgcgcgaaacagacaagccggtaaagttgttctacggctcaagaactccgcgcgacgctgttcggtggatcgatatcgacatcgatgaggacaagcttgaggtcgtccaggcagttacggaagacacggatagcctttggcaagggcccactggttttattcatcaggttgtcgacgcagcgctgcttgaaaccctaccggaatacgaaatttatcttgccggtccaccgcctatggtcgacgctactgtccgtatgctgctcggcaagggtgttccacgcgatcaaattcattttgacgcatttttctaa BBa_K902034_sequence 1 atgtaccaactcaaaattgaagggcaagcgccagggacctgcggctcagggaagagcctgttggtctcagcacttgctaatggtatcggatttccgtacgagtgtgcatcgggaggttgcggagtatgcaaattcgagttactcgaagggaatgtccaatcaatgtggccggatgctccaggactttcttcgcgagatcgtgagaagggcaaccgccatcttgcatgccagtgcgttgcgctctcagacctgcggatcaaagtcgcagtgcaggacaagtacgtcccaacgattccaatctcaagaatggaagcggaagttgttgaggtccgggcgctaactcatgacctgctgtccgtgcgattacgcactgatgggccagcaaatttcctccccggccagttctgcctagtagaggcagagcagttgccaggcgtggttcgcgcatattcaatggcgaatttaaagaaccccgaaggcatatgggagttctatattaagagggtacccacaggacgatttagtccttggcttttcgaaaatagaaaagaaggcgctcgtctatttttgacgggaccaatgggcacatctttcttccgtccagggaccggccgaaagagtctttgcattggcggcggtgccgggctctcgtatgcagccgctattgcacgcgcctcgatgcgcgaaacagacaagccggtaaagttgttctacggctcaagaactccgcgcgacgctgttcggtggatcgatatcgacatcgatgaggacaagcttgaggtcgtccaggcagttacggaagacacggatagcctttggcaagggcccactggttttattcatcaggttgtcgacgcagcgctgcttgaaaccctaccggaatacgaaatttatcttgccggtccaccgcctatggtcgacgctactgtccgtatgctgctcggcaagggtgttccacgcgatcaaattcattttgacgcatttttctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z