BBa_K902051 1 BBa_K902051 dszA behind and RBS (B0034) 2012-09-30T11:00:00Z 2015-05-08T01:13:43Z Amplified from an environmental isolate of R. erythropolis DszA behind an RBS false false _1167_ 0 13399 9 It's complicated false See DszA mutations false Lisa Oberding, Ali Honarmand component2205125 1 BBa_K902050 component2205118 1 BBa_B0034 annotation2205125 1 BBa_K902050 range2205125 1 19 1380 annotation2205118 1 BBa_B0034 range2205118 1 1 12 BBa_K902050 1 dszA DBT-sulfone monooxygenase (DszA) from R. erythropolis 2012-09-30T11:00:00Z 2015-05-08T01:13:43Z Obtained from PCR amplification from a plasmid found in an environmental isolate of R. erythropolis shown to be an active desulfurizer. A monooxygenase enzyme involved in the 4S desulfurization pathway. This protein encoded by this sequence carries out the third step in the pathway, converting DBT-sulfone to 2-hydroxybiphenyl-2-sulfinic acid through addition of an oxygen to the sulfur heteroatom. FMNH2 is consumed by this reaction. false false _1167_ 0 13399 9 It's complicated false 4 internal PstI cut-sites had to be mutated with site-directed mutagenesis. false Lisa Oberding, Ali Honarmand annotation2205052 1 G->A range2205052 1 639 639 annotation2205093 1 DszA range2205093 1 1 1362 annotation2205051 1 start range2205051 1 1 3 annotation2205054 1 G->A range2205054 1 1011 1011 annotation2205055 1 G->A range2205055 1 1266 1266 annotation2205053 1 G->A range2205053 1 678 678 annotation2211180 1 stop range2211180 1 1360 1362 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K902051_sequence 1 aaagaggagaaatactagatgactcaacaacgacaaatgcatctggccggtttcttctcggccggcaatgtgactcatgcacatggggcgtggcggcacacggacgcgtcgaatgactttctgtcggggaagtactaccaacacatcgcccgtactctggagcgcggcaagttcgatctgttgtttctgcctgacgggttggccgtcgaggacagctacggggacaacctggacaccggtgtcggcctgggcgggcagggtgcagtcgccttggagccggccagtgtggtcgcaaccatggccgcggtgaccgagcacctgggtcttggggcaaccatttcggcgacctactatcccccgtatcacgttgctcgggtgttcgcgacgctcgatcagttgtcagggggtcgggtgtcctggaacgtcgtcacctcgctcaacgacgctgaagcgcgcaacttcggcattaatcagcatctggaacacgacgcccgctatgaccgcgccgatgagttcttggaagcggtcaagaaactctggaacagctgggacgaggacgccctcgtgctggacaaggcggccggcgtgttcgccgatcccgcgaaggtgcactacgtcgatcaccacggggagtggctgaatgtgcgcggacctctacaggtaccgcgttcacctcagggtgagccggtgatcctacaggccggcctgtcgccccggggtcggcgcttcgccgggaagtgggccgaggccgtcttcagtcttgcacccaacctcgaggtgatgcaggccacctaccagggcatcaaagccgaggtcgacgctgcggggcgcgatcccgatcagacgaaaatcttcaccgccgtgatgccggtactcggcgaaagccaggcggtggcacaggaacgactggaatatctcaacagtctggtccatccggaagtgggactgtcgacgctatccagtcacaccggcatcaacctggcggcgtaccctctcgacactccgatcaaggacatcctgcgggatctacaggatcggaatgtcccgacgcaactgcacatgttcgccgccgcaacgcacagcgaagagctcacgctggcggaaatgggtcggcgctatggaaccaacgtggggttcgttcctcagtgggccggtaccggggagcagatcgctgacgagctgatccgccacttcgagggcggcgccgcggatggtttcatcatctctccggccttcctgccgggctcctacgacgagttcgtcgaccaggtggttccggttctacaggatcgcggctacttccgcaccgagtaccagggcaacactctgcgcgaccacttgggtctgcgcgtaccacaactgcaaggacaaccttcatga BBa_B0034_sequence 1 aaagaggagaaa BBa_K902050_sequence 1 atgactcaacaacgacaaatgcatctggccggtttcttctcggccggcaatgtgactcatgcacatggggcgtggcggcacacggacgcgtcgaatgactttctgtcggggaagtactaccaacacatcgcccgtactctggagcgcggcaagttcgatctgttgtttctgcctgacgggttggccgtcgaggacagctacggggacaacctggacaccggtgtcggcctgggcgggcagggtgcagtcgccttggagccggccagtgtggtcgcaaccatggccgcggtgaccgagcacctgggtcttggggcaaccatttcggcgacctactatcccccgtatcacgttgctcgggtgttcgcgacgctcgatcagttgtcagggggtcgggtgtcctggaacgtcgtcacctcgctcaacgacgctgaagcgcgcaacttcggcattaatcagcatctggaacacgacgcccgctatgaccgcgccgatgagttcttggaagcggtcaagaaactctggaacagctgggacgaggacgccctcgtgctggacaaggcggccggcgtgttcgccgatcccgcgaaggtgcactacgtcgatcaccacggggagtggctgaatgtgcgcggacctctacaggtaccgcgttcacctcagggtgagccggtgatcctacaggccggcctgtcgccccggggtcggcgcttcgccgggaagtgggccgaggccgtcttcagtcttgcacccaacctcgaggtgatgcaggccacctaccagggcatcaaagccgaggtcgacgctgcggggcgcgatcccgatcagacgaaaatcttcaccgccgtgatgccggtactcggcgaaagccaggcggtggcacaggaacgactggaatatctcaacagtctggtccatccggaagtgggactgtcgacgctatccagtcacaccggcatcaacctggcggcgtaccctctcgacactccgatcaaggacatcctgcgggatctacaggatcggaatgtcccgacgcaactgcacatgttcgccgccgcaacgcacagcgaagagctcacgctggcggaaatgggtcggcgctatggaaccaacgtggggttcgttcctcagtgggccggtaccggggagcagatcgctgacgagctgatccgccacttcgagggcggcgccgcggatggtttcatcatctctccggccttcctgccgggctcctacgacgagttcgtcgaccaggtggttccggttctacaggatcgcggctacttccgcaccgagtaccagggcaacactctgcgcgaccacttgggtctgcgcgtaccacaactgcaaggacaaccttcatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z