##gff-version 3 ##sequence-region BBa_K902054 1 1324 BBa_K902054 . engineered_region 1 220 . + 0 ID=BBa_J04500;Name=BBa_J04500 BBa_K902054 . ribosome_entry_site 209 220 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500 BBa_K902054 . conserved_region 213 216 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K902054 . promoter 1 200 . + 0 ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500 BBa_K902054 . non_covalent_binding_site 166 200 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_K902054 . non_covalent_binding_site 89 126 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_K902054 . engineered_region 1 200 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_K902054 . promoter 137 142 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_K902054 . CDS 1 88 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_K902054 . start_codon 173 173 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_K902054 . promoter 161 166 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_K902054 . CDS 227 1324 . + 0 ID=BBa_K902052;Name=BBa_K902052 BBa_K902054 . start_codon 227 229 . + 0 ID=annotation2205133;Name=start;Parent=BBa_K902052 BBa_K902054 . sequence_alteration 565 565 . + 0 ID=annotation2205135;Name=G->A;Parent=BBa_K902052 BBa_K902054 . CDS 227 1324 . + 0 ID=annotation2205132;Name=DszB;Parent=BBa_K902052 BBa_K902054 . stop_codon 1322 1324 . + 0 ID=annotation2211179;Name=stop;Parent=BBa_K902052 BBa_K902054 . sequence_alteration 413 415 . + 0 ID=annotation2205134;Name=Y63F;Parent=BBa_K902052 BBa_K902054 . sequence_alteration 1252 1252 . + 0 ID=annotation2205136;Name=G->A;Parent=BBa_K902052 >BBa_K902054 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacaagccg cgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtaccc aacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagg gcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggtt gcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagc ccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagc tgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgca cacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgct gagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttgg ccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggt ggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggc cttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgc acgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacg cctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtc gccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatag