BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K902056
1
BBa_K902056
dszC behind an RBS (B0034)
2012-10-01T11:00:00Z
2015-05-08T01:13:43Z
amplified from a Rhodococcus environmental isolate
DszC with B0034. Built in collaboration with the University of British Colombia iGEM Team.
false
false
_1167_
0
13399
9
It's complicated
false
Mutagenesis
false
Lisa Oberding, Ali Honarmand
component2210966
1
BBa_B0034
component2210967
1
BBa_K804005
annotation2210967
1
BBa_K804005
range2210967
1
19
1272
annotation2210966
1
BBa_B0034
range2210966
1
1
12
BBa_K804005
1
BBa_K804005
DszC coding gene (from DszABC operon)
2012-09-30T11:00:00Z
2015-05-08T01:13:25Z
http://www.ncbi.nlm.nih.gov/nuccore/L37363.1 (GenBank: L37363.1)
This is a DszC coding gene from the DszABC operon in the 4S pathway of Rhodococcus erythropolis IGTS8. It encodes one of the three biodesulfurizing enzymes in the DszABC operon. DszC enzymes have been shown to catalyze dibenzothiophene (DBT)to dibenzothiophene-5-oxide (DBTO) in the first reaction and then from DBTO to DBT sulfoxide (DBTO2) in the second reaction, both in the presence of NADH, FMN and flavin reductases.
false
false
_1060_
0
9774
9
It's complicated
false
2 PstI sites were removed using site-directed mutagenesis
false
Joe Ho
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K902056_sequence
1
aaagaggagaaatactagatgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttccacggacttccacaacgtcaaggtcgagcctgacgaagtgctgggcgcgcccaacgccttcgttctcgccttcatacaatccgagcgcggcagcctcttccggcccatagcgcaattgatcttcgccaacgtctatctggggatcgcgcacggcgcactcgatgccgccagggagtacacccgtacccaggcgaggccctggacaccggccggtattcaacaggcaaccgaggatccctacaccatccgctcctacggtgagttcaccatcgcattgcagggagctgacgccgccgcccgtgaagcggcccacctgctacagacggtgtgggacaagggcgacgcgctcacccccgaggaccgcggcgaactgatggtgaaggtctcgggagtcaaagcgttggccaccaacgccgccctcaacatcagcagcggcgtcttcgaggtgatcggcgcgcgcggaacacatcccaggtacggtttcgaccgcttctggcgcaacgtgcgcacccactccctgcacgacccggtgtcctacaagatcgccgacgtcggcaagcacaccttgaacggtcaatacccgattcccggcttcacctcctga
BBa_K804005_sequence
1
atgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttccacggacttccacaacgtcaaggtcgagcctgacgaagtgctgggcgcgcccaacgccttcgttctcgccttcatacaatccgagcgcggcagcctcttccggcccatagcgcaattgatcttcgccaacgtctatctggggatcgcgcacggcgcactcgatgccgccagggagtacacccgtacccaggcgaggccctggacaccggccggtattcaacaggcaaccgaggatccctacaccatccgctcctacggtgagttcaccatcgcattgcagggagctgacgccgccgcccgtgaagcggcccacctgctacagacggtgtgggacaagggcgacgcgctcacccccgaggaccgcggcgaactgatggtgaaggtctcgggagtcaaagcgttggccaccaacgccgccctcaacatcagcagcggcgtcttcgaggtgatcggcgcgcgcggaacacatcccaggtacggtttcgaccgcttctggcgcaacgtgcgcacccactccctgcacgacccggtgtcctacaagatcgccgacgtcggcaagcacaccttgaacggtcaatacccgattcccggcttcacctcctga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z