LOCUS BBa_K902062 3525 bp DNA linear 22-Nov-2024 DEFINITION CAR (Nocardia carboxylic acid reductase) ACCESSION BBa_K902062 VERSION BBa_K902062.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=ATG start codon misc_feature 3523..3525 /label=TGA stop codon misc_feature 1764..1764 /label=PstI Mutagenized Site 2 (G to A) misc_feature 846..846 /label=PstI Mutagenized Site 1 (G to C) misc_feature 3220..3220 /label=EcorI Mutagenized Site 1 (T to C) ORIGIN 1 atggcagtgg attcaccgga tgagcggcta cagcgccgca ttgcacagtt gtttgcagaa 61 gatgagcagg tcaaggccgc acgtccgctc gaagcggtga gcgcggcggt gagcgcgccc 121 ggtatgcggc tggcgcagat cgccgccact gttatggcgg gttacgccga ccgcccggcc 181 gccgggcagc gtgcgttcga actgaacacc gacgacgcga cgggccgcac ctcgctgcgg 241 ttacttcccc gattcgagac catcacctat cgcgaactgt ggcagcgagt cggcgaggtt 301 gccgcggcct ggcatcatga tcccgagaac cccttgcgcg caggtgattt cgtcgccctg 361 ctcggcttca ccagcatcga ctacgccacc ctcgacctgg ccgatatcca cctcggcgcg 421 gttaccgtgc cgttgcaggc cagcgcggcg gtgtcccagc tgatcgctat cctcaccgag 481 acttcgccgc ggctgctcgc ctcgaccccg gagcacctcg atgcggcggt cgagtgccta 541 ctcgcgggca ccacaccgga acgactggtg gtcttcgact accaccccga ggacgacgac 601 cagcgtgcgg ccttcgaatc cgcccgccgc cgccttgccg acgcgggcag cttggtgatc 661 gtcgaaacgc tcgatgccgt gcgtgcccgg ggccgcgact taccggccgc gccactgttc 721 gttcccgaca ccgacgacga cccgctggcc ctgctgatct acacctccgg cagcaccgga 781 acgccgaagg gcgcgatgta caccaatcgg ttggccgcca cgatgtggca ggggaactcg 841 atgctccagg ggaactcgca acgggtcggg atcaatctca actacatgcc gatgagccac 901 atcgccggtc gcatatcgct gttcggcgtg ctcgctcgcg gtggcaccgc atacttcgcg 961 gccaagagcg acatgtcgac actgttcgaa gacatcggct tggtacgtcc caccgagatc 1021 ttcttcgtcc cgcgcgtgtg cgacatggtc ttccagcgct atcagagcga gctggaccgg 1081 cgctcggtgg cgggcgccga cctggacacg ctcgatcggg aagtgaaagc cgacctccgg 1141 cagaactacc tcggtgggcg cttcctggtg gcggtcgtcg gcagcgcgcc gctggccgcg 1201 gagatgaaga cgttcatgga gtccgtcctc gatctgccac tgcacgacgg gtacgggtcg 1261 accgaggcgg gcgcaagcgt gctgctcgac aaccagatcc agcggccgcc ggtgctcgat 1321 tacaagctcg tcgacgtgcc cgaactgggt tacttccgca ccgaccggcc gcatccgcgc 1381 ggtgagctgt tgttgaaggc ggagaccacg attccgggct actacaagcg gcccgaggtc 1441 accgcggaga tcttcgacga ggacggcttc tacaagaccg gcgatatcgt ggccgagctc 1501 gagcacgatc ggctggtcta tgtcgaccgt cgcaacaatg tgctcaaact gtcgcagggc 1561 gagttcgtga ccgtcgccca tctcgaggcc gtgttcgcca gcagcccgct gatccggcag 1621 atcttcatct acggcagcag cgaacgttcc tatctgctcg cggtgatcgt ccccaccgac 1681 gacgcgctgc gcggccgcga caccgccacc ttgaaatcgg cactggccga atcgattcag 1741 cgcatcgcca aggacgcgaa cctacagccc tacgagattc cgcgcgattt cctgatcgag 1801 accgagccgt tcaccatcgc caacggactg ctctccggca tcgcgaagct gctgcgcccc 1861 aatctgaagg aacgctacgg cgctcagctg gagcagatgt acaccgatct cgcgacaggc 1921 caggccgatg agctgctcgc cctgcgccgc gaagccgccg acctgccggt gctcgaaacc 1981 gtcagccggg cagcgaaagc gatgctcggc gtcgcctccg ccgatatgcg tcccgacgcg 2041 cacttcaccg acctgggcgg cgattccctt tccgcgctgt cgttctcgaa cctgctgcac 2101 gagatcttcg gggtcgaggt gccggtgggt gtcgtcgtca gcccggcgaa cgagctgcgc 2161 gatctggcga attacattga ggcggaacgc aactcgggcg cgaagcgtcc caccttcacc 2221 tcggtgcacg gcggcggttc cgagatccgc gccgccgatc tgaccctcga caagttcatc 2281 gatgcccgca ccctggccgc cgccgacagc attccgcacg cgccggtgcc agcgcagacg 2341 gtgctgctga ccggcgcgaa cggctacctc ggccggttcc tgtgcctgga atggctggag 2401 cggctggaca agacgggtgg cacgctgatc tgcgtcgtgc gcggtagtga cgcggccgcg 2461 gcccgtaaac ggctggactc ggcgttcgac agcggcgatc ccggcctgct cgagcactac 2521 cagcaactgg ccgcacggac cctggaagtc ctcgccggtg atatcggcga cccgaatctc 2581 ggtctggacg acgcgacttg gcagcggttg gccgaaaccg tcgacctgat cgtccatccc 2641 gccgcgttgg tcaaccacgt ccttccctac acccagctgt tcggccccaa tgtcgtcggc 2701 accgccgaaa tcgtccggtt ggcgatcacg gcgcggcgca agccggtcac ctacctgtcg 2761 accgtcggag tggccgacca ggtcgacccg gcggagtatc aggaggacag cgacgtccgc 2821 gagatgagcg cggtgcgcgt cgtgcgcgag agttacgcca acggctacgg caacagcaag 2881 tgggcggggg aggtcctgct gcgcgaagca cacgatctgt gtggcttgcc ggtcgcggtg 2941 ttccgttcgg acatgatcct ggcgcacagc cggtacgcgg gtcagctcaa cgtccaggac 3001 gtgttcaccc ggctgatcct cagcctggtc gccaccggca tcgcgccgta ctcgttctac 3061 cgaaccgacg cggacggcaa ccggcagcgg gcccactatg acggcttgcc ggcggacttc 3121 acggcggcgg cgatcaccgc gctcggcatc caagccaccg aaggcttccg gacctacgac 3181 gtgctcaatc cgtacgacga tggcatctcc ctcgatgaac tcgtcgactg gctcgtcgaa 3241 tccggccacc cgatccagcg catcaccgac tacagcgact ggttccaccg tttcgagacg 3301 gcgatccgcg cgctgccgga aaagcaacgc caggcctcgg tgctgccgtt gctggacgcc 3361 taccgcaacc cctgcccggc ggtccgcggc gcgatactcc cggccaagga gttccaagcg 3421 gcggtgcaaa cagccaaaat cggtccggaa caggacatcc cgcatttgtc cgcgccactg 3481 atcgataagt acgtcagcga tctggaactg cttcagctgc tctga //