BBa_K902079
type: ComponentDefinition
persistent identity: BBa K902079
display id: BBa_K902079
version: 1
was generated by: iGEM to SBOL conversion
description: PtetR/dszB/B0034/dszC
created: 2012-10-24T11:00:00Z
modified: 2015-05-08T01:13:44Z
mutable provenance: na
owned by: james
owned by: myers
mutable description: J13/DszB/B0034/DszC
discontinued: false
dominant: false
experience: experience/None
group u list: _1167_
m user id: 0
owner id: 13399
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: na
star: false
creator: Lisa Oberding, Ali Honarmand
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K902056
component: BBa_K902053
sequence annotation: BBa_K902053
sequence annotation: BBa_K902056
sequence: https://synbiohub.org/public/igem/BBa_K902079_sequence/1

BBa_K902056
type: Component
persistent identity: component2212790
display id: component2212790
version: 1
top level: BBa_K902079
definition: BBa_K902056
access: public

BBa_K902053
type: Component
persistent identity: component2212786
display id: component2212786
version: 1
top level: BBa_K902079
definition: BBa_K902053
access: public

BBa_K902053
type: SequenceAnnotation
persistent identity: annotation2212786
display id: annotation2212786
version: 1
top level: BBa_K902079
location: https://synbiohub.org/public/igem/BBa_K902079/annotation2212786/range2212786/1
component: BBa_K902053

https://synbiohub.org/public/igem/BBa_K902079/annotation2212786/range2212786/1
type: Range
persistent identity: range2212786
display id: range2212786
version: 1
top level: BBa_K902079
direction: direction/reverse
start: 1
end: 1178
orientation: inline

BBa_K902056
type: SequenceAnnotation
persistent identity: annotation2212790
display id: annotation2212790
version: 1
top level: BBa_K902079
location: https://synbiohub.org/public/igem/BBa_K902079/annotation2212790/range2212790/1
component: BBa_K902056

https://synbiohub.org/public/igem/BBa_K902079/annotation2212790/range2212790/1
type: Range
persistent identity: range2212790
display id: range2212790
version: 1
top level: BBa_K902079
direction: direction/reverse
start: 1187
end: 2458
orientation: inline

BBa_K902053
type: ComponentDefinition
persistent identity: BBa K902053
display id: BBa_K902053
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive DszB generator (J13002-DszB)
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:43Z
mutable provenance: DszB amplified from an environmental isolate of Rhodococcus that is an active desulfurizer, promotor from registry.
owned by: james
owned by: myers
mutable description: DszB constitutive expression
discontinued: false
dominant: false
experience: experience/None
group u list: _1167_
m user id: 0
owner id: 13399
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: See DszB mutations
star: false
creator: Lisa Oberding, Ali Honarmand
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K902052
component: BBa_J13002
sequence annotation: BBa_J13002
sequence annotation: BBa_K902052
sequence: https://synbiohub.org/public/igem/BBa_K902053_sequence/1

BBa_K902052
type: Component
persistent identity: component2205151
display id: component2205151
version: 1
top level: BBa_K902053
definition: dszB
access: public

BBa_J13002
type: Component
persistent identity: component2205145
display id: component2205145
version: 1
top level: BBa_K902053
definition: BBa_J13002
access: public

BBa_J13002
type: SequenceAnnotation
persistent identity: annotation2205145
display id: annotation2205145
version: 1
top level: BBa_K902053
location: https://synbiohub.org/public/igem/BBa_K902053/annotation2205145/range2205145/1
component: BBa_J13002

https://synbiohub.org/public/igem/BBa_K902053/annotation2205145/range2205145/1
type: Range
persistent identity: range2205145
display id: range2205145
version: 1
top level: BBa_K902053
direction: direction/reverse
start: 1
end: 74
orientation: inline

BBa_K902052
type: SequenceAnnotation
persistent identity: annotation2205151
display id: annotation2205151
version: 1
top level: BBa_K902053
location: https://synbiohub.org/public/igem/BBa_K902053/annotation2205151/range2205151/1
component: BBa_K902052

https://synbiohub.org/public/igem/BBa_K902053/annotation2205151/range2205151/1
type: Range
persistent identity: range2205151
display id: range2205151
version: 1
top level: BBa_K902053
direction: direction/reverse
start: 81
end: 1178
orientation: inline

BBa_K902056
type: ComponentDefinition
persistent identity: BBa K902056
display id: BBa_K902056
version: 1
was generated by: iGEM to SBOL conversion
description: dszC behind an RBS (B0034)
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:43Z
mutable provenance: amplified from a Rhodococcus environmental isolate
owned by: james
owned by: myers
mutable description: DszC with B0034. Built in collaboration with the University of British Colombia iGEM Team.
discontinued: false
dominant: false
experience: experience/None
group u list: _1167_
m user id: 0
owner id: 13399
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Mutagenesis
star: false
creator: Lisa Oberding, Ali Honarmand
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_K804005
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K804005
sequence: https://synbiohub.org/public/igem/BBa_K902056_sequence/1

BBa_K804005
type: Component
persistent identity: component2210967
display id: component2210967
version: 1
top level: BBa_K902056
definition: BBa_K804005
access: public

BBa_B0034
type: Component
persistent identity: component2210966
display id: component2210966
version: 1
top level: BBa_K902056
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2210966
display id: annotation2210966
version: 1
top level: BBa_K902056
location: https://synbiohub.org/public/igem/BBa_K902056/annotation2210966/range2210966/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K902056/annotation2210966/range2210966/1
type: Range
persistent identity: range2210966
display id: range2210966
version: 1
top level: BBa_K902056
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K804005
type: SequenceAnnotation
persistent identity: annotation2210967
display id: annotation2210967
version: 1
top level: BBa_K902056
location: https://synbiohub.org/public/igem/BBa_K902056/annotation2210967/range2210967/1
component: BBa_K804005

https://synbiohub.org/public/igem/BBa_K902056/annotation2210967/range2210967/1
type: Range
persistent identity: range2210967
display id: range2210967
version: 1
top level: BBa_K902056
direction: direction/reverse
start: 19
end: 1272
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_J13002
type: ComponentDefinition
persistent identity: BBa J13002
display id: BBa_J13002
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressed POPS/RIPS generator
created: 2005-06-15T11:00:00Z
modified: 2015-08-31T04:08:29Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _37_5_
m user id: 0
owner id: 88
owning group id: 37
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Jeff Tabor
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J13002_sequence/1

BBa_B0034
type: Component
persistent identity: component1535786
display id: component1535786
version: 1
top level: BBa_J13002
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1535778
display id: component1535778
version: 1
top level: BBa_J13002
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1535778
display id: annotation1535778
version: 1
top level: BBa_J13002
location: https://synbiohub.org/public/igem/BBa_J13002/annotation1535778/range1535778/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J13002/annotation1535778/range1535778/1
type: Range
persistent identity: range1535778
display id: range1535778
version: 1
top level: BBa_J13002
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1535786
display id: annotation1535786
version: 1
top level: BBa_J13002
location: https://synbiohub.org/public/igem/BBa_J13002/annotation1535786/range1535786/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J13002/annotation1535786/range1535786/1
type: Range
persistent identity: range1535786
display id: range1535786
version: 1
top level: BBa_J13002
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_K804005
type: ComponentDefinition
persistent identity: BBa K804005
display id: BBa_K804005
version: 1
was generated by: iGEM to SBOL conversion
description: DszC coding gene (from DszABC operon)
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:25Z
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/L37363.1 (GenBank: L37363.1)
owned by: james
owned by: myers
mutable description: This is a DszC coding gene from the DszABC operon in the 4S pathway of Rhodococcus erythropolis IGTS8. It encodes one of the three biodesulfurizing enzymes in the DszABC operon. DszC enzymes have been shown to catalyze dibenzothiophene (DBT)to dibenzothiophene-5-oxide (DBTO) in the first reaction and then from DBTO to DBT sulfoxide (DBTO2) in the second reaction, both in the presence of NADH, FMN and flavin reductases.
discontinued: false
dominant: false
group u list: _1060_
m user id: 0
owner id: 9774
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: 2 PstI sites were removed using site-directed mutagenesis
star: false
creator: Joe Ho
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K804005_sequence/1

dszB
type: ComponentDefinition
persistent identity: BBa K902052
display id: BBa_K902052
version: 1
was generated by: iGEM to SBOL conversion
description: HBPS desulfinase (DszB) from R. erythropolis (mutated for improved activity)
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:43Z
mutable provenance: Amplified from a plasmid in an environmental isolate of Rhodococcus shown to be an active desulfurizer.
owned by: james
owned by: myers
mutable description: DszB - HBPS desulfinase which carries out the last step of the 4S pathway, converting 2-hydroxybiphenyl-2-sulfinic acid (HBPS) to 2-hydroxybiphenyl. The sequence has been mutated Y63F to increase enzyme activity.
discontinued: false
dominant: false
experience: experience/None
group u list: _1167_
m user id: 0
owner id: 13399
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Mutations of internal PstI and NotI cut sites in order to make biobrick compatible, as well as an added mutation of Y63F in the protein to increase its activity.
star: false
creator: Lisa Oberding, Ali Honarmand
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: DszB
sequence annotation: start
sequence annotation: Y63F
sequence annotation: G->A
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K902052_sequence/1

DszB
type: SequenceAnnotation
persistent identity: annotation2205132
display id: annotation2205132
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2205132/range2205132/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K902052/annotation2205132/range2205132/1
type: Range
persistent identity: range2205132
display id: range2205132
version: 1
top level: dszB
direction: direction/forward
start: 1
end: 1098
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2205133
display id: annotation2205133
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2205133/range2205133/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K902052/annotation2205133/range2205133/1
type: Range
persistent identity: range2205133
display id: range2205133
version: 1
top level: dszB
direction: direction/forward
start: 1
end: 3
orientation: inline

Y63F
type: SequenceAnnotation
persistent identity: annotation2205134
display id: annotation2205134
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2205134/range2205134/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K902052/annotation2205134/range2205134/1
type: Range
persistent identity: range2205134
display id: range2205134
version: 1
top level: dszB
direction: direction/forward
start: 187
end: 189
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation2205135
display id: annotation2205135
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2205135/range2205135/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K902052/annotation2205135/range2205135/1
type: Range
persistent identity: range2205135
display id: range2205135
version: 1
top level: dszB
direction: direction/forward
start: 339
end: 339
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation2205136
display id: annotation2205136
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2205136/range2205136/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K902052/annotation2205136/range2205136/1
type: Range
persistent identity: range2205136
display id: range2205136
version: 1
top level: dszB
direction: direction/forward
start: 1026
end: 1026
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2211179
display id: annotation2211179
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2211179/range2211179/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K902052/annotation2211179/range2211179/1
type: Range
persistent identity: range2211179
display id: range2211179
version: 1
top level: dszB
direction: direction/forward
start: 1096
end: 1098
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

https://synbiohub.org/public/igem/BBa_K902079_sequence/1
type: Sequence
persistent identity: BBa K902079 sequence
display id: BBa_K902079_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatagtactagagaaagaggagaaatactagatgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttccacggacttccacaacgtcaaggtcgagcctgacgaagtgctgggcgcgcccaacgccttcgttctcgccttcatacaatccgagcgcggcagcctcttccggcccatagcgcaattgatcttcgccaacgtctatctggggatcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K902053_sequence/1
type: Sequence
persistent identity: BBa K902053 sequence
display id: BBa_K902053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K902056_sequence/1
type: Sequence
persistent identity: BBa K902056 sequence
display id: BBa_K902056_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttccacggacttccacaacgtcaaggtcgagcctgacgaagtgctgggcgcgcccaacgccttcgttctcgccttcatacaatccgagcgcggcagcctcttccggcccatagcgcaattgatcttcgccaacgtctatctggggatcgcgcacggcgcactcgatgccgccagggagtacacccgtacccaggcgaggccctggacaccggccggtattcaacaggcaaccgaggatccctacaccatccgctcctacggtgagttcaccatcgcattgcagggagctgacgccgccgcccgtgaagcggcccacctgctacagacggtgtgggacaagggcgacgcgctcacccccgaggaccgcggcgaactgatggtgaaggtctcgggagtcaaagcgttggccaccaacgccgccctcaacatcagcagcggcgtcttcgaggtgatcggcgcgcgcggaacacatcccaggtacggtttcgaccgcttctggcgcaacgtgcgcacccactccctgcacgacccggtgtcctacaagatcgccgacgtcggcaagcacaccttgaacggtcaatacccgattcccggcttcacctcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J13002_sequence/1
type: Sequence
persistent identity: BBa J13002 sequence
display id: BBa_J13002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K804005_sequence/1
type: Sequence
persistent identity: BBa K804005 sequence
display id: BBa_K804005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttccacggacttccacaacgtcaaggtcgagcctgacgaagtgctgggcgcgcccaacgccttcgttctcgccttcatacaatccgagcgcggcagcctcttccggcccatagcgcaattgatcttcgccaacgtctatctggggatcgcgcacggcgcactcgatgccgccagggagtacacccgtacccaggcgaggccctggacaccggccggtattcaacaggcaaccgaggatccctacaccatccgctcctacggtgagttcaccatcgcattgcagggagctgacgccgccgcccgtgaagcggcccacctgctacagacggtgtgggacaagggcgacgcgctcacccccgaggaccgcggcgaactgatggtgaaggtctcgggagtcaaagcgttggccaccaacgccgccctcaacatcagcagcggcgtcttcgaggtgatcggcgcgcgcggaacacatcccaggtacggtttcgaccgcttctggcgcaacgtgcgcacccactccctgcacgacccggtgtcctacaagatcgccgacgtcggcaagcacaccttgaacggtcaatacccgattcccggcttcacctcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K902052_sequence/1
type: Sequence
persistent identity: BBa K902052 sequence
display id: BBa_K902052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z