Constitutive DszB generator (J13002-DszB)
DszB amplified from an environmental isolate of Rhodococcus that is an active desulfurizer, promotor from registry.
amplified from a Rhodococcus environmental isolate
DszC with B0034. Built in collaboration with the University of British Colombia iGEM Team.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
http://www.ncbi.nlm.nih.gov/nuccore/L37363.1 (GenBank: L37363.1)
This is a DszC coding gene from the DszABC operon in the 4S pathway of Rhodococcus erythropolis IGTS8. It encodes one of the three biodesulfurizing enzymes in the DszABC operon. DszC enzymes have been shown to catalyze dibenzothiophene (DBT)to dibenzothiophene-5-oxide (DBTO) in the first reaction and then from DBTO to DBT sulfoxide (DBTO2) in the second reaction, both in the presence of NADH, FMN and flavin reductases.
2 PstI sites were removed using site-directed mutagenesis
HBPS desulfinase (DszB) from R. erythropolis (mutated for improved activity)
Amplified from a plasmid in an environmental isolate of Rhodococcus shown to be an active desulfurizer.
DszB - HBPS desulfinase which carries out the last step of the 4S pathway, converting 2-hydroxybiphenyl-2-sulfinic acid (HBPS) to 2-hydroxybiphenyl. The sequence has been mutated Y63F to increase enzyme activity.
Mutations of internal PstI and NotI cut sites in order to make biobrick compatible, as well as an added mutation of Y63F in the protein to increase its activity.
Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
Sequence for pTet inverting regulator driven by the TetR protein.
BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
Conversion of the iGEM parts registry to SBOL2.1