BBa_K902081
type: ComponentDefinition
persistent identity: BBa K902081
display id: BBa_K902081
version: 1
was generated by: iGEM to SBOL conversion
description: 4S Desulfurization Pathway Optimization Circuit (PlacI/hpaC/RBS/katGLAA)
created: 2012-10-24T11:00:00Z
modified: 2015-05-08T01:13:44Z
mutable provenance: na
owned by: james
owned by: myers
mutable description: J04/H/Kat
discontinued: false
dominant: false
experience: experience/None
group u list: _1167_
m user id: 0
owner id: 13399
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: na
star: false
creator: Lisa Oberding, Ali Honarmand
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K902059
component: BBa_K902058
sequence annotation: BBa_K902058
sequence annotation: BBa_K902059
sequence: https://synbiohub.org/public/igem/BBa_K902081_sequence/1

BBa_K902059
type: Component
persistent identity: component2212828
display id: component2212828
version: 1
top level: BBa_K902081
definition: BBa_K902059
access: public

BBa_K902058
type: Component
persistent identity: component2212823
display id: component2212823
version: 1
top level: BBa_K902081
definition: BBa_K902058
access: public

BBa_K902058
type: SequenceAnnotation
persistent identity: annotation2212823
display id: annotation2212823
version: 1
top level: BBa_K902081
location: https://synbiohub.org/public/igem/BBa_K902081/annotation2212823/range2212823/1
component: BBa_K902058

https://synbiohub.org/public/igem/BBa_K902081/annotation2212823/range2212823/1
type: Range
persistent identity: range2212823
display id: range2212823
version: 1
top level: BBa_K902081
direction: direction/reverse
start: 1
end: 739
orientation: inline

BBa_K902059
type: SequenceAnnotation
persistent identity: annotation2212828
display id: annotation2212828
version: 1
top level: BBa_K902081
location: https://synbiohub.org/public/igem/BBa_K902081/annotation2212828/range2212828/1
component: BBa_K902059

https://synbiohub.org/public/igem/BBa_K902081/annotation2212828/range2212828/1
type: Range
persistent identity: range2212828
display id: range2212828
version: 1
top level: BBa_K902081
direction: direction/reverse
start: 748
end: 2982
orientation: inline

BBa_K902058
type: ComponentDefinition
persistent identity: BBa K902058
display id: BBa_K902058
version: 1
was generated by: iGEM to SBOL conversion
description: hpaC inducible generator with lacI promotor (J04500)
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:43Z
mutable provenance: Escherichia coli W
owned by: james
owned by: myers
mutable description: HpaC protein under control of the lacI promotor (J04500).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1167_
m user id: 0
owner id: 13399
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None
star: false
creator: Lisa Oberding, Ali Honarmand
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K902057
component: BBa_J04500
sequence annotation: BBa_J04500
sequence annotation: BBa_K902057
sequence: https://synbiohub.org/public/igem/BBa_K902058_sequence/1

BBa_K902057
type: Component
persistent identity: component2205105
display id: component2205105
version: 1
top level: BBa_K902058
definition: hpaC
access: public

BBa_J04500
type: Component
persistent identity: component2205102
display id: component2205102
version: 1
top level: BBa_K902058
definition: BBa_J04500
access: public

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2205102
display id: annotation2205102
version: 1
top level: BBa_K902058
location: https://synbiohub.org/public/igem/BBa_K902058/annotation2205102/range2205102/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K902058/annotation2205102/range2205102/1
type: Range
persistent identity: range2205102
display id: range2205102
version: 1
top level: BBa_K902058
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_K902057
type: SequenceAnnotation
persistent identity: annotation2205105
display id: annotation2205105
version: 1
top level: BBa_K902058
location: https://synbiohub.org/public/igem/BBa_K902058/annotation2205105/range2205105/1
component: BBa_K902057

https://synbiohub.org/public/igem/BBa_K902058/annotation2205105/range2205105/1
type: Range
persistent identity: range2205105
display id: range2205105
version: 1
top level: BBa_K902058
direction: direction/reverse
start: 227
end: 739
orientation: inline

BBa_K902059
type: ComponentDefinition
persistent identity: BBa K902059
display id: BBa_K902059
version: 1
was generated by: iGEM to SBOL conversion
description: katG +LAA behind an RBS (B0034)
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:43Z
mutable provenance: Registry
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: KatG +LAA behind and RBS site (B0034)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1167_
m user id: 0
owner id: 13399
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: false
creator: Lisa Oberding, Ali Honarmand
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_K137068
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K137068
sequence: https://synbiohub.org/public/igem/BBa_K902059_sequence/1

BBa_K137068
type: Component
persistent identity: component2205170
display id: component2205170
version: 1
top level: BBa_K902059
definition: katG
access: public

BBa_B0034
type: Component
persistent identity: component2205168
display id: component2205168
version: 1
top level: BBa_K902059
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2205168
display id: annotation2205168
version: 1
top level: BBa_K902059
location: https://synbiohub.org/public/igem/BBa_K902059/annotation2205168/range2205168/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K902059/annotation2205168/range2205168/1
type: Range
persistent identity: range2205168
display id: range2205168
version: 1
top level: BBa_K902059
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K137068
type: SequenceAnnotation
persistent identity: annotation2205170
display id: annotation2205170
version: 1
top level: BBa_K902059
location: https://synbiohub.org/public/igem/BBa_K902059/annotation2205170/range2205170/1
component: BBa_K137068

https://synbiohub.org/public/igem/BBa_K902059/annotation2205170/range2205170/1
type: Range
persistent identity: range2205170
display id: range2205170
version: 1
top level: BBa_K902059
direction: direction/reverse
start: 19
end: 2235
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

katG
type: ComponentDefinition
persistent identity: BBa K137068
display id: BBa_K137068
version: 1
was generated by: iGEM to SBOL conversion
description: katG +LAA
created: 2008-07-30T11:00:00Z
modified: 2015-05-08T01:10:09Z
mutable provenance: Genomic PCR off of DH10B
owned by: james
owned by: myers
mutable description: This is one of two catalases from E. coli. It has a C-term LAA ssrA tag.
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Site directed mutagenesis to remove an EcoRI site.
star: false
creator: Doug Tischer
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: C->T
sequence: https://synbiohub.org/public/igem/BBa_K137068_sequence/1

C->T
type: SequenceAnnotation
persistent identity: annotation1969499
display id: annotation1969499
version: 1
top level: katG
location: https://synbiohub.org/public/igem/BBa_K137068/annotation1969499/range1969499/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K137068/annotation1969499/range1969499/1
type: Range
persistent identity: range1969499
display id: range1969499
version: 1
top level: katG
direction: direction/forward
start: 198
end: 198
orientation: inline

hpaC
type: ComponentDefinition
persistent identity: BBa K902057
display id: BBa_K902057
version: 1
was generated by: iGEM to SBOL conversion
description: HpaC- 4-hydroxyphenylacetate 3-monooxygenase reductase subunit
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:43Z
mutable provenance: Escherichia coli W
owned by: james
owned by: myers
mutable description: Recycles FMNH2 utilizing NADH and FMN.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1167_
m user id: 0
owner id: 13399
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: false
creator: Lisa Oberding, Ali Honarmand
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start
sequence annotation: HpaC
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K902057_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2205091
display id: annotation2205091
version: 1
top level: hpaC
location: https://synbiohub.org/public/igem/BBa_K902057/annotation2205091/range2205091/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K902057/annotation2205091/range2205091/1
type: Range
persistent identity: range2205091
display id: range2205091
version: 1
top level: hpaC
direction: direction/forward
start: 1
end: 3
orientation: inline

HpaC
type: SequenceAnnotation
persistent identity: annotation2205092
display id: annotation2205092
version: 1
top level: hpaC
location: https://synbiohub.org/public/igem/BBa_K902057/annotation2205092/range2205092/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K902057/annotation2205092/range2205092/1
type: Range
persistent identity: range2205092
display id: range2205092
version: 1
top level: hpaC
direction: direction/forward
start: 1
end: 513
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2211176
display id: annotation2211176
version: 1
top level: hpaC
location: https://synbiohub.org/public/igem/BBa_K902057/annotation2211176/range2211176/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K902057/annotation2211176/range2211176/1
type: Range
persistent identity: range2211176
display id: range2211176
version: 1
top level: hpaC
direction: direction/forward
start: 511
end: 513
orientation: inline

BBa_J04500
type: ComponentDefinition
persistent identity: BBa J04500
display id: BBa_J04500
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG inducible promoter with RBS
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: R0010.B0034
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Kristen DeCelle
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04500_sequence/1

BBa_B0034
type: Component
persistent identity: component1508159
display id: component1508159
version: 1
top level: BBa_J04500
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component1508149
display id: component1508149
version: 1
top level: BBa_J04500
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1508149
display id: annotation1508149
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
type: Range
persistent identity: range1508149
display id: range1508149
version: 1
top level: BBa_J04500
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1508159
display id: annotation1508159
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
type: Range
persistent identity: range1508159
display id: range1508159
version: 1
top level: BBa_J04500
direction: direction/forward
start: 209
end: 220
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

https://synbiohub.org/public/igem/BBa_K902081_sequence/1
type: Sequence
persistent identity: BBa K902081 sequence
display id: BBa_K902081_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcaattagatgaacaacgcctgcgctttcgtgacgcaatggccagcctgtcggcagcggtaaatattatcaccaccgagggcgacgccggacaatgcgggattacggcaacggccgtctgctcggtcacggatacaccaccatcgctgatggtgtgcattaacgccaacagtgcgatgaacccggtttttcagggcaacggtaagttgtgcgtcaacgtcctcaaccatgagcaggaactgatggcacgccacttcgcgggcatgacaggcatggcgatggaagagcgttttagcctctcatgctggcaaaaaggtccgctggcgcagccggtgctaaaaggttcgctggccagtcttgaaggtgagatccgcgatgtgcaggcaattggcacacatctggtgtatctggtggagattaaaaacatcatcctcagtgcagaaggtcacggacttatctactttaaacgccgtttccatccggtgatgctggaaatggaagctgcgatttaatactagagaaagaggagaaatactagatgagcacgtcagacgatatccataacaccacagccactggcaaatgcccgttccatcagggcggtcacgaccagagtgcgggggcgggcacaaccactcgcgactggtggccaaatcaacttcgtgttgacctgttaaaccaacattctaatcgttctaacccactgggtgaggactttgactaccgcaaagaatttagcaaattagattactacggcctgaaaaaagatctgaaagccctgttgacagaatctcaaccgtggtggccagccgactggggcagttacgccggtctgtttattcgtatggcctggcacggcgcggggacttaccgttcaatcgatggacgcggtggcgcgggtcgtggtcagcaacgttttgcaccgctgaactcctggccggataacgtaagcctcgataaagcgcgtcgcctgttgtggccaatcaaacagaaatatggtcagaaaatctcctgggccgacctgtttatcctcgcgggtaacgtggcgctagaaaactccggcttccgtaccttcggttttggtgccggtcgtgaagacgtctgggaaccggatctggatgttaactggggtgatgaaaaagcctggctgactcaccgtcatccggaagcgctggcgaaagcaccgctgggtgcaaccgagatgggtctgatttacgttaacccggaaggcccggatcacagcggcgaaccgctttctgcggcagcagctatccgcgcgaccttcggcaacatgggcatgaacgacgaagaaaccgtggcgctgattgcgggtggtcatacgctgggtaaaacccacggtgccggtccgacatcaaatgtaggtcctgatccagaagctgcaccgattgaagaacaaggtttaggttgggcgagcacttacggcagcggcgttggcgcagatgccattacctctggtctggaagtagtctggacccagacgccgacccagtggagcaactatttcttcgagaacctgttcaagtatgagtgggtacagacccgcagcccggctggcgcaatccagttcgaagcggtagacgcaccggaaattatcccggatccgtttgatccgtcgaagaaacgtaaaccgacaatgctggtgaccgacctgacgctgcgttttgatcctgagttcgagaagatctctcgtcgtttcctcaacgatccgcaggcgttcaacgaagcctttgcccgtgcctggttcaaactgac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K902058_sequence/1
type: Sequence
persistent identity: BBa K902058 sequence
display id: BBa_K902058_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcaattagatgaacaacgcctgcgctttcgtgacgcaatggccagcctgtcggcagcggtaaatattatcaccaccgagggcgacgccggacaatgcgggattacggcaacggccgtctgctcggtcacggatacaccaccatcgctgatggtgtgcattaacgccaacagtgcgatgaacccggtttttcagggcaacggtaagttgtgcgtcaacgtcctcaaccatgagcaggaactgatggcacgccacttcgcgggcatgacaggcatggcgatggaagagcgttttagcctctcatgctggcaaaaaggtccgctggcgcagccggtgctaaaaggttcgctggccagtcttgaaggtgagatccgcgatgtgcaggcaattggcacacatctggtgtatctggtggagattaaaaacatcatcctcagtgcagaaggtcacggacttatctactttaaacgccgtttccatccggtgatgctggaaatggaagctgcgatttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K902059_sequence/1
type: Sequence
persistent identity: BBa K902059 sequence
display id: BBa_K902059_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagcacgtcagacgatatccataacaccacagccactggcaaatgcccgttccatcagggcggtcacgaccagagtgcgggggcgggcacaaccactcgcgactggtggccaaatcaacttcgtgttgacctgttaaaccaacattctaatcgttctaacccactgggtgaggactttgactaccgcaaagaatttagcaaattagattactacggcctgaaaaaagatctgaaagccctgttgacagaatctcaaccgtggtggccagccgactggggcagttacgccggtctgtttattcgtatggcctggcacggcgcggggacttaccgttcaatcgatggacgcggtggcgcgggtcgtggtcagcaacgttttgcaccgctgaactcctggccggataacgtaagcctcgataaagcgcgtcgcctgttgtggccaatcaaacagaaatatggtcagaaaatctcctgggccgacctgtttatcctcgcgggtaacgtggcgctagaaaactccggcttccgtaccttcggttttggtgccggtcgtgaagacgtctgggaaccggatctggatgttaactggggtgatgaaaaagcctggctgactcaccgtcatccggaagcgctggcgaaagcaccgctgggtgcaaccgagatgggtctgatttacgttaacccggaaggcccggatcacagcggcgaaccgctttctgcggcagcagctatccgcgcgaccttcggcaacatgggcatgaacgacgaagaaaccgtggcgctgattgcgggtggtcatacgctgggtaaaacccacggtgccggtccgacatcaaatgtaggtcctgatccagaagctgcaccgattgaagaacaaggtttaggttgggcgagcacttacggcagcggcgttggcgcagatgccattacctctggtctggaagtagtctggacccagacgccgacccagtggagcaactatttcttcgagaacctgttcaagtatgagtgggtacagacccgcagcccggctggcgcaatccagttcgaagcggtagacgcaccggaaattatcccggatccgtttgatccgtcgaagaaacgtaaaccgacaatgctggtgaccgacctgacgctgcgttttgatcctgagttcgagaagatctctcgtcgtttcctcaacgatccgcaggcgttcaacgaagcctttgcccgtgcctggttcaaactgacgcacagggatatggggccgaaatctcgctacatcgggccggaagtgccgaaagaagatctgatctggcaagatccgctgccgcagccgatctacaacccgaccgagcaggacattatcgatctgaaattcgcgattgcggattctggtctgtctgttagtgagctggtatcggtggcctgggcatctgcttctaccttccgtggtggcgacaaacgcggtggtgccaacggtgcgcgtctggcattaatgccgcagcgcgactgggatgtgaacgccgcagccgttcgtgctctgcctgttctggagaaaatccagaaagagtctggtaaagcctcgctggcggatatcatagtgctggctggtgtggttggtgttgagaaagccgcaagcgccgcaggtttgagcattcatgtaccgtttgcgccgggtcgcgttgatgcgcgtcaggatcagactgacattgagatgtttgagctgctggagccaattgctgacggtttccgtaactatcgcgctcgtctggacgtttccaccaccgagtcactgctgatcgacaaagcacagcaactgacgctgaccgcgccggaaatgactgcgctggtgggcggcatgcgtgtactgggtgccaacttcgatggcagcaaaaacggcgtcttcactgaccgcgttggcgtattgagcaatgacttcttcgtgaacttgctggatatgcgttacgagtggaaagcgaccgacgaatcgaaaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K137068_sequence/1
type: Sequence
persistent identity: BBa K137068 sequence
display id: BBa_K137068_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacgtcagacgatatccataacaccacagccactggcaaatgcccgttccatcagggcggtcacgaccagagtgcgggggcgggcacaaccactcgcgactggtggccaaatcaacttcgtgttgacctgttaaaccaacattctaatcgttctaacccactgggtgaggactttgactaccgcaaagaatttagcaaattagattactacggcctgaaaaaagatctgaaagccctgttgacagaatctcaaccgtggtggccagccgactggggcagttacgccggtctgtttattcgtatggcctggcacggcgcggggacttaccgttcaatcgatggacgcggtggcgcgggtcgtggtcagcaacgttttgcaccgctgaactcctggccggataacgtaagcctcgataaagcgcgtcgcctgttgtggccaatcaaacagaaatatggtcagaaaatctcctgggccgacctgtttatcctcgcgggtaacgtggcgctagaaaactccggcttccgtaccttcggttttggtgccggtcgtgaagacgtctgggaaccggatctggatgttaactggggtgatgaaaaagcctggctgactcaccgtcatccggaagcgctggcgaaagcaccgctgggtgcaaccgagatgggtctgatttacgttaacccggaaggcccggatcacagcggcgaaccgctttctgcggcagcagctatccgcgcgaccttcggcaacatgggcatgaacgacgaagaaaccgtggcgctgattgcgggtggtcatacgctgggtaaaacccacggtgccggtccgacatcaaatgtaggtcctgatccagaagctgcaccgattgaagaacaaggtttaggttgggcgagcacttacggcagcggcgttggcgcagatgccattacctctggtctggaagtagtctggacccagacgccgacccagtggagcaactatttcttcgagaacctgttcaagtatgagtgggtacagacccgcagcccggctggcgcaatccagttcgaagcggtagacgcaccggaaattatcccggatccgtttgatccgtcgaagaaacgtaaaccgacaatgctggtgaccgacctgacgctgcgttttgatcctgagttcgagaagatctctcgtcgtttcctcaacgatccgcaggcgttcaacgaagcctttgcccgtgcctggttcaaactgacgcacagggatatggggccgaaatctcgctacatcgggccggaagtgccgaaagaagatctgatctggcaagatccgctgccgcagccgatctacaacccgaccgagcaggacattatcgatctgaaattcgcgattgcggattctggtctgtctgttagtgagctggtatcggtggcctgggcatctgcttctaccttccgtggtggcgacaaacgcggtggtgccaacggtgcgcgtctggcattaatgccgcagcgcgactgggatgtgaacgccgcagccgttcgtgctctgcctgttctggagaaaatccagaaagagtctggtaaagcctcgctggcggatatcatagtgctggctggtgtggttggtgttgagaaagccgcaagcgccgcaggtttgagcattcatgtaccgtttgcgccgggtcgcgttgatgcgcgtcaggatcagactgacattgagatgtttgagctgctggagccaattgctgacggtttccgtaactatcgcgctcgtctggacgtttccaccaccgagtcactgctgatcgacaaagcacagcaactgacgctgaccgcgccggaaatgactgcgctggtgggcggcatgcgtgtactgggtgccaacttcgatggcagcaaaaacggcgtcttcactgaccgcgttggcgtattgagcaatgacttcttcgtgaacttgctggatatgcgttacgagtggaaagcgaccgacgaatcgaaagagctgttcgaaggccgtga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K902057_sequence/1
type: Sequence
persistent identity: BBa K902057 sequence
display id: BBa_K902057_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaattagatgaacaacgcctgcgctttcgtgacgcaatggccagcctgtcggcagcggtaaatattatcaccaccgagggcgacgccggacaatgcgggattacggcaacggccgtctgctcggtcacggatacaccaccatcgctgatggtgtgcattaacgccaacagtgcgatgaacccggtttttcagggcaacggtaagttgtgcgtcaacgtcctcaaccatgagcaggaactgatggcacgccacttcgcgggcatgacaggcatggcgatggaagagcgttttagcctctcatgctggcaaaaaggtccgctggcgcagccggtgctaaaaggttcgctggccagtcttgaaggtgagatccgcgatgtgcaggcaattggcacacatctggtgtatctggtggagattaaaaacatcatcctcagtgcagaaggtcacggacttatctactttaaacgccgtttccatccggtgatgctggaaatggaagctgcgatttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04500_sequence/1
type: Sequence
persistent identity: BBa J04500 sequence
display id: BBa_J04500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z