LOCUS       BBa_K902082             1181 bp    DNA     linear       01-Jun-2024
DEFINITION  TetR Promoter-moco riboswitch-GFP LacI Promoter-rbs
ACCESSION   BBa_K902082
VERSION     BBa_K902082.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     promoter        63..191
                     /label=BBa_K902023
     misc_feature    198..917
                     /label=GFP
     misc_feature    918..950
                     /label=LVA
     CDS             198..953
                     /label=BBa_K082003
     CDS             962..1049
                     /label=end of LacI coding region (inactive)
     misc_feature    962..1161
                     /label=BBa_R0010
     misc_feature    1050..1087
                     /label=CAP binding site
     promoter        1098..1103
                     /label=-35
     promoter        1122..1127
                     /label=-10
     misc_feature    1127..1161
                     /label=LacI binding site
     misc_feature    1134..1134
                     /label=start
     promoter        962..1161
                     /label=BBa_R0010
     misc_feature    1174..1177
                     /label=conserved
     RBS             1170..1181
                     /label=BBa_B0034
     misc_feature    962..1181
                     /label=BBa_J04500
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 aggggaacca ctaaacacct agcctctgca cctgggtcaa ctgatacggt gctttggccg
      121 tgacaatgct cgtaaagatt gccaccaggg cgaaggaaga aatgacttcg cctcccgtat
      181 ctggaaaggt gtactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc
      241 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      301 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      361 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc
      421 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      481 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
      541 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
      601 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
      661 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
      721 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
      781 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
      841 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
      901 aactatacaa aaggcctgct gcaaacgacg aaaactacgc tttagtagct taatactaga
      961 gcaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     1021 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     1081 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     1141 agcggataac aatttcacac atactagaga aagaggagaa a
//