LOCUS BBa_K902083 3827 bp DNA linear 16-Dec-2024 DEFINITION R0040-moco-S7-J04500-moaoperon ACCESSION BBa_K902083 VERSION BBa_K902083.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 promoter 63..191 /label=BBa_K902023 misc_feature 509..509 /label=See Design Considerations CDS 198..890 /label=BBa_K902019 CDS 899..986 /label=end of LacI coding region (inactive) misc_feature 899..1098 /label=BBa_R0010 misc_feature 987..1024 /label=CAP binding site promoter 1035..1040 /label=-35 promoter 1059..1064 /label=-10 misc_feature 1064..1098 /label=LacI binding site misc_feature 1071..1071 /label=start promoter 899..1098 /label=BBa_R0010 misc_feature 1111..1114 /label=conserved RBS 1107..1118 /label=BBa_B0034 misc_feature 899..1118 /label=BBa_J04500 misc_feature 1125..2114 /label=moaA CDS 1125..3827 /label=BBa_K902024 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 aggggaacca ctaaacacct agcctctgca cctgggtcaa ctgatacggt gctttggccg 121 tgacaatgct cgtaaagatt gccaccaggg cgaaggaaga aatgacttcg cctcccgtat 181 ctggaaaggt gtactagatg acagagtatc tcctaagcgc gggcatctgt atggccattg 241 tttcgatctt attgatcggc atggcgatat cgaacgtatc taagggccag tacgcgaaac 301 gcttcttctt tttcgccacg agttgccttg tcttaacatt agttgttgtt tcttcactgt 361 cgtccagtgc gaacgcctct caaaccgata acggtgtaaa tcgcagcggg agcgaagatc 421 ctaccgtgta ttccgcgacc tcaacgaaaa aacttcataa ggagcccgct acgctcatca 481 aagccattga cggagacact gtaaagctga tgtacaaagg ccaaccgatg acctttcgct 541 tgctgctggt cgacacccca gagacgaaac atccgaaaaa gggcgtcgag aaatacggcc 601 cggaagcaag tgcgtttacc aagaagatgg tggaaaatgc gaacaaaatt gaagtcgaat 661 ttgataaagg gcagcgtacc gacaaatatg gtcgtggact ggcctacatt tatgctgatg 721 gtaaaatggt gaatgaagca ctggtgcggc agggtctggc caaagtggct tatgtgtata 781 aaccgaataa tactcacgaa cagctgctgc gtaaatccga agcacaggca aaaaaagaaa 841 aactgaacat ttggagcgaa gataacgcgg atagcggtca ataataataa tactagagca 901 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 961 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 1021 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 1081 ggataacaat ttcacacata ctagagaaag aggagaaata ctagatggct tcacaactga 1141 ctgatgcatt tgcgcgtaag ttttactact tgcgcctgtc gattaccgat gtgtgtaact 1201 ttcgttgcac ctactgcctg ccggatggct acaaaccgag cggcgtcacc aataaaggct 1261 ttcttaccgt cgatgaaatt cgccgggtta cgcgcgcctt cgccagactg ggcaccgaaa 1321 aagtgcgcct gacaggagga gagccgtctt tacgccgcga ctttaccgat atcatcgccg 1381 ctgtgcggga aaacgacgct atccgccaga ttgcggtcac aaccaatggt taccgtctgg 1441 aacgcgatgt ggcgagctgg cgcgatgcgg gacttactgg cattaacgtc agtgtcgaca 1501 gtctggacgc ccgccagttt cacgctatta ccgggcagga taaattcaac caggtcatgg 1561 cagggattga tgctgcattt gaggccggtt ttgagaaggt caaagtcaat accgtgctga 1621 tgcgtgatgt taatcatcac cagctcgaca cctttctgaa ctggatccag catcgcccta 1681 tccagctgcg tttcatcgaa ctgatggaaa cgggcgaggg cagcgagctc ttccgtaagc 1741 atcacatctc tggtcaggtt ctgcgtgacg agctactgcg tcgcggctgg atccaccaat 1801 tacgtcaacg cagcgacggt cccgcgcaag tcttttgcca tccagattac gccggagaga 1861 ttggccttat catgccgtat gaaaaagact tctgcgccac ttgcaaccgc ctgcgcgttt 1921 cctccattgg taaactccat ctctgcctgt ttggtgaagg cggcgttaac ctgcgcgatc 1981 tgctggaaga cgatacccag caacaggcgc tggaagcgcg tatttcagcg gcgctgcggg 2041 agaagaaaca gacccatttc ctgcatcaaa acaacaccgg tattacgcaa aacttatcgt 2101 acattggcgg ctaaaacgtc aaaaggagag atcagatgag tcaggtaagc actgaattta 2161 tcccgacccg tattgctatt cttacggttt ctaatcgtcg cggtgaagaa gacgatacct 2221 ccggtcacta tctgcgcgat tcggcgcaag aagcgggcca tcacgttgtc gataaagcca 2281 ttgtgaaaga aaaccgctac gctattcgcg ctcaggtatc tgcgtggatc gccagcgacg 2341 atgtacaagt ggtattgatt acgggtggta ctggcctgac ggaaggtgat caggctcccg 2401 aagcattgct gccgttgttc gaccgtgaag ttgaaggttt tggtgaagtg ttccggatgt 2461 tgtcgtttga agagattggc acttccacgt tgcaatctcg tgcggtagcg ggcgtcgcca 2521 acaaaacgct gattttcgcc atgccgggtt cgaccaaagc gtgccgtacc gcatgggaaa 2581 atatcatcgc gccgcagctg gatgcccgta cgcgtccgtg taatttccat ccacatttga 2641 agaaataagt atgtcgcaac tgacccatat caacgccgct ggcgaagcgc acatggtgga 2701 tgtctccgcc aaagcggaaa ccgtgcgtga agcgcgggcg gaagcctttg tcaccatgcg 2761 cagcgagacg ctggcgatga ttattgatgg tcgccaccac aaaggcgacg tatttgccac 2821 tgcgcgtatt gccggtattc aggcggcaaa acgcacctgg gatctgatcc cgctctgtca 2881 tccgctgatg ctcagcaaag ttgaagtcaa tttacaggcc gagccggagc acaatcgggt 2941 gcgtatagaa accttatgcc gcctgaccgg gaaaaccggt gtcgaaatgg aagcattaac 3001 cgcggcctcc gtggcggcgc tgaccattta tgacatgtgc aaagcggtgc aaaaagatat 3061 ggtgattggt ccggtacgtt tgctggcgaa gagcggcggc aagtcgggtg actttaaggt 3121 ggaagcggat gattaaagtt ctttttttcg cccaggtgcg cgagttggtg ggaacagatg 3181 caaccgaagt ggctgcggat ttcccaactg ttgaagcgtt acgccagcac atggctgcgc 3241 agagcgatcg ctgggcgctg gcgctggaag atggcaaatt actggctgcc gtcaaccaga 3301 cgctggtgag ttttgaccat ccgctgactg acggcgacga agtagctttc ttcccgccgg 3361 taaccggagg ttaagatggc agaaaccaaa attgttgttg gtccgcagcc gttcagcgta 3421 ggagaagagt acccgtggct ggcggagcgt gacgaagacg gtgcggtagt cacctttact 3481 ggtaaggtgc gcaaccataa cctgggcgac agcgtcaacg cattaaccct cgaacactat 3541 ccggggatga ctgaaaaagc actggcagaa attgttgatg aagcgcgtaa ccgctggccg 3601 ctggggcgcg tcactgtgat tcaccgcatc ggggaattat ggccgggcga tgaaatcgtt 3661 tttgtcggtg tcaccagtgc gcatcgcagc agtgcgtttg aagccgggca gtttattatg 3721 gattatctca aaacccgcgc accgttctgg aagcgcgaag ccacgccgga aggcgaccgc 3781 tgggttgaag ctcgggagag cgatcagcag gcggcaaaac gctggta //